Functional analysis of the ACAT-1-positive late endosomes in cholesterol-rich macrophages
| Project/Area Number |
20590384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Kumamoto University |
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Principal Investigator |
SAKASHITA Naomi Kumamoto University, 大学院・生命科学研究部, 准教授 (90284752)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEYA Motohiro 熊本大学, 大学院・生命科学研究部, 教授 (90155052)
KOMOHARA Yoshihiro 熊本大学, 大学院・生命科学研究部, 助教 (40449921)
FUJIWARA Yukio 熊本大学, 大学院・生命科学研究部, 助教 (70452886)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 細胞 / マクロファージ / コレステロール / 異物貪食 / ACAT-1 / ファゴゾーム / 後期エンドゾーム / NPC1 / オルガネラ / ACAT1 |
|---|
| Research Abstract |
We functionally analyzed ACAT1-positive late endosomes (ACAT1-LE) which appears in cholesterol-rich macrophages. ACAT1-LE contributed effective cholesterol esterification, in addition, they showed impaired phagosomal maturation. Interestingly, such impairment partially correlated by pharmacological inhibition of cholesterol esterification.
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Report
(4 results)
Research Products
(103 results)
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[Journal Article] Partial impairment of c-Ret at tyrosine 1062 accelerates age-related hearing loss in mice.2011
Author(s)
Ohgami N, Ida-Eto M, Sakashita N, Sone M, Makashima T, Tabuchi K, Hoshino T, Shimada A, Tsuzuki T, Yamamoto M, Sobue G, Jijiwa M, Asai N, Hara A, Takashasi M, Kato M.
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Journal Title
Neurobiology of Aging in press
Related Report
Peer Reviewed
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[Journal Article] Natural Compounds Containing a Catechol Group Enhance the Formation of N(ε)-(carboxymethyl)lysine of the Maillard Reaction.2010
Author(s)
Fujiwara Y, Kiyota N, Tsurushima K, Yoshitomi M, Mera K, Sakashita N, Takeya M, Ikeda T, Araki T, Nohara T, Nagai R
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Journal Title
Free Radical Biological Medicine 50(7)
Pages: 883-891
Related Report
Peer Reviewed
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[Journal Article] c-Ret-mediated hearing loss in mice with Hirschsprung disease.2010
Author(s)
Ohgami N, Ida-Eto M, Shimotake T, Sakashita N, Sone M, Nakashima T, Tabuchi K, Hoshino T, Shimada A, Tsuzuki T, Yamamoto M, Sobue G, Jijiwa M, Asai N, Hara A, Takahashi M, Kato M
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Journal Title
Proceedings of the National Academy of Sciences of the United States of America. 107(29)
Pages: 13501-13506
Related Report
Peer Reviewed
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[Journal Article] Pioglitazone, a peroxisome proliferator-activated receptor-g agonist, attenuates myocardial ischemia-reperfusion injury in mice with metabolic disorders.2008
Author(s)
Honda T, Kaikita K, Tsujita K, Hayasaki T, Matsukawa M, Fuchigami S, Sugiyama S, Sakashita N, Ogawa H, Takeya M
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Journal Title
Journal of molecular cell cardiology 44(5)
Pages: 915-926
Related Report
Peer Reviewed
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[Journal Article] Specific pathogen free conditions prevent transthyretin amyloidosis in mouse models.2008
Author(s)
Inoue S, Ohta M, Li Z, Zhao G, Takaoka Y, Sakashita N, Miyakawa K, Takada K, Tei H, Suzuki M, Masuoka M, Sakaki Y, Takahashi K, Yamamura KI
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Journal Title
Transgenic Research 17(5)
Pages: 817-826
Related Report
Peer Reviewed
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