Verification of hypoxia-reoxygenation as one of an internal carcinogenic factor for epithelium
Project/Area Number |
20590393
|
Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Experimental pathology
|
Research Institution | Yamagata University |
Principal Investigator |
OKADA Futoshi Yamagata University, 医学部, 教授 (00250423)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 低酸素再酸素化 / 発癌 / 上皮細胞 |
Research Abstract |
I examined whether a series of ischemia-reperfusion condition could act on normal epithelial cells as an endogenous carcinogenic factor. I used three epithelial cell lines to examine whether those cell lines could be converted into tumorigenic ones after cultivation in a series of hypoxia-reoxygenation condition, i.e., between the incubators kept at 1% and 20% O_2 concentrations. The cell lines were sporadically converted into tumorigenic ones in nude mice after serial long passages. I also examined whether a series of ischemia-reperfusion condition could be carcinogenic by using mouse kidney ; so far I have not detected any tumors in the ischemia-reperfusion organ. I need to examine possible conversion using another organ.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] Development of a quantitative bioassay to assess preventive compounds against inflammation-based carcinogenesis.2011
Author(s)
Onuma K, Suenaga Y, Sakaki R, Yoshitome S, Sato Y, Ogawara S, Suzuki S, Kuramitsu M, Yokoyama H, Murakami A, Hamada J, Nicolson GL, Kobayashi M, Fujii J, Okada F.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Spontaneous skin damage and delayed wound healing in SOD1-deficient mice.2010
Author(s)
Iuchi Y, Roy D, Okada F, Kibe N, Tsunoda S, Suzuki S, Takahashi M, Yokoyama H, Yoshitake J, Kondo S, Fujii J.
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Journal Title
Mot Cell Biochem 341
Pages: 181-194
Related Report
Peer Reviewed
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[Journal Article] High-intensity focused ultrasound induced apoptosis with caspase 3, 8, and 9/6 activation in rat hepatoma.2009
Author(s)
Hirokawa N, Koito K, Okada F, Kudo N, Yamamoto K, Fujimoto K, Nishida M, Ichimura T, Hori M, Satoh T, Hareyama M.
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Journal Title
J Med Ultrasonics 36
Pages: 177-185
NAID
Related Report
Peer Reviewed
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[Journal Article] Rescue of anemia and autoimmune responses in SOD1-deficient mice by transgenic expression of human SOD1 in erythrocytes.2009
Author(s)
Iuchi Y, Okada F, Takamiya R, Kibe N, Tsunoda S, Nakajima O, Toyoda K, Nagae R, Suematsu M, Soga T, Uchida K, Fujii J.
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Journal Title
Biochem J 422
Pages: 313-320
Related Report
Peer Reviewed
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