Analysis of basic mechanisms of osteoclastogenesis to apply to clinical study

• Project/Area Number: 20590400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Tottori University
• Principal Investigator: HAYASHI Shin-ichi Tottori University, 医学部, 教授 (50208617)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 炎症 / 破骨細胞 / 免疫学 / 骨吸収 / 発生・分化 / 病理学 / 再生医学

Research Abstract

Bones are resorbed both in the steady and pathological states ; however, it is not clear whether the regulation of osteoclastogenesis is same. Here, I detected the presence of the regulation of bone resorption from extramedullary sites. Intramedullary T lineage cells but also the cells in the peritoneal cavity might control the osteoclastogenesis. These results suggest that the novel therapy for bone diseases such as osteoporosis or arthritis will be opened.

Research Products

• [Journal Article] HLA-A2-restricted glypican-3 peptide-specific CTL clones induced by peptide vaccine show high avidity and antigen-specific killing activity against tumor cells. (2011)
  • Author(s): Yoshikawa, T., Nakatsugawa, M., Suzuki, S., Shirakawa, H., Nobuoka, D., Sakemura, N., Motomura, Y., Tanaka-Harada, Y., Hayashi, S.I., Nakatsura, T.
  • Journal Title: Cancer Sci. 102 Pages: 918-925
  • NAID: 10029293106
• [Journal Article] HLA-A2-restricted glypican-3 peptide-specific CTL clones induced by peptide vaccine show high avidity and antigen-specific killing activity against tumor cells (2011)
  • Author(s): Yoshikawa, T., et al.
  • Journal Title: Cancer Sci. Volume: 102 Pages: 918-925
  • Peer Reviewed
• [Journal Article] A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells. (2010)
  • Author(s): Murata, A., Okuyama, K., Sakano, S., Kajiki, M., Hirata, T., Yagita, H., Zuniga-Pflucker, J.C., Miyake, K., Akashi-Takamura, S., Moriwaki, S., Niida, S., Yoshino, M., Hayashi, S.I.
  • Journal Title: J.Immunol. 185 Pages: 3905-3912
• [Journal Article] Delta-like 1 functions as an adhesion molecule for mast cells (2010)
  • Author(s): Murata, A., et al.
  • Journal Title: J.Immunol. Volume: 185 Pages: 3905-3912
  • Peer Reviewed
• [Journal Article] Comparison of osteoclast precursors in peripheral blood mononuclear cells from rheumatoid arthritis and osteoporosis patients. (2009)
  • Author(s): Nose, M., Yamazaki, H., Hagino, H., Morio, Y., Hayashi, S.I., Teshima, R.
  • Journal Title: J.Bone Miner.Metab. 27 Pages: 57-65
  • NAID: 10025336201
• [Journal Article] Comparison of osteoclast precursors in peripheral blood mononuclear cells from rheumatoid arthritis and osteoporosis patients. (2009)
  • Author(s): Nose, M., et al.
  • Journal Title: J.Bone Miner.Metab. VOL.27 Pages: 57-65
  • Peer Reviewed
• [Journal Article] Comparison of osteoclast precursors in peripheral blood mononuclear cells from rheumatoid arthritis and osteoporosis patients. (2009)
  • Author(s): Nose, M., et al.
  • Journal Title: J. Bone Miner. Metab. 27 Pages: 57-65
  • Peer Reviewed
• [Journal Article] Suppression of macrophage functions impairs skeletal muscle regeneration with severe fibrosis. (2008)
  • Author(s): Segawa, M., Fukada, S., Yamamoto, Y., Yahagi, H., Kanematsu, M., Sato, M., Ito, T., Uezumi, A., Hayashi, S.I., Miyagoe-Suzuki, Y., Takeda, S., Tsujikawa, K., Yamamoto, H.
  • Journal Title: Exp.Cell Res. 314 Pages: 3232-3244
• [Journal Article] Isolation and characterization of Kit-independent melanocyte precursors induced in the skin of Steel factor transgenic mice. (2008)
  • Author(s): Kawaguchi, A., Chiba, K., Tanimura, Y., Motohashi, T., Aoki, H., Takeda, T., Hayashi, S.I., Shimizu, K., Kunisada, T.
  • Journal Title: Develop. Growth Differ. 50 Pages: 63-69
  • NAID: 10021095452
• [Journal Article] Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. (2008)
  • Author(s): Yoshino, M., Yamazaki, H., Hayashi, S.I.
  • Journal Title: Immunol. Lett. 115 Pages: 131-137
• [Journal Article] Analysis of capturing skin antigens in the steady state using milk fat globule EGF factor 8-deficient skin-hyperpigmented mice. (2008)
  • Author(s): Yoshino, M., et al.
  • Journal Title: Immunoi. Lett. 116 Pages: 131-137
  • Peer Reviewed
• [Journal Article] Isolation and characterization of Kit-independent melanocyte precursors induced in the skin of Steel factor transgenic mice. (2008)
  • Author(s): Kawaguchi, A., et al.
  • Journal Title: Develop. Growth Differ. 50 Pages: 63-69
  • Peer Reviewed
• [Journal Article] Suppression of macrophage functions impairs skeletal muscle regeneration with severe fibrosis. (2008)
  • Author(s): Segawa, M., et al.
  • Journal Title: Exp. Cell Res. 314 Pages: 3232-3244
  • Peer Reviewed
• [Presentation] Distinct mechanisms of self-antigen transport from the epidermis and the dermis to regional lymph nodes (2010)
  • Author(s): Yoshino, M., et al.
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 神戸コンベンションセンター(神戸)
  • Year and Date: 2010-08-24
• [Presentation] Detection of bone-marrow precursors given rise to B-lineage cells in the presence of delta-like 1 (2010)
  • Author(s): Okuyama, K., et al.
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 神戸コンベンションセンター(神戸)
  • Year and Date: 2010-08-24
• [Presentation] Distinct mechanisms of self-antigen transport from the epidermis and the dermis to regional lymph nodes. (2010)
  • Author(s): Yoshino, M., Okuyama, K., Murata, A., Egawa, Y., Sawada, A., Hayashi, S.I.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Detection of bone-marrow precursors given rise to B-lineage cells in the presence of delta-like 1. (2010)
  • Author(s): Okuyama, K., Murata, A., Egawa, Y., Sawada, A., Yoshino, M., Hayashi, S.I.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Function of Delta-like 1 as an adhesion molecule. (2010)
  • Author(s): Murata, A., Okuyama, K., Sawada, A., Egawa, Y., Sakano, S., Yagita, H., Zuniga-Pflucker, J.C., Yoshino, M., Hayashi, S.I.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] CCR7-independent trafficking of skin antigens to regional lymph nodes by cells in the dermis. (2010)
  • Author(s): Yoshino, M., Okuyama, K., Murata, A., Egawa, Y., Sawada, A., Hayashi, S.I.
  • Organizer: 18^<th> International Symposium on Molecular Cell Biology of Macrophages 2010 : Macrophage Activation and Disease Process.
  • Place of Presentation: 熊本
• [Presentation] Function of Delta-like 1 as an adhesion molecule (2010)
  • Author(s): Murata, A., et al.
  • Organizer: 第14回国際免疫学会議
  • Place of Presentation: 神戸コンベンションセンター(神戸)
• [Presentation] CCR7-independent trafficking of skin antigens to regional lymphnodes by cells in the dermis (2010)
  • Author(s): Yoshino, M., et al.
  • Organizer: 第18回マクロファージ分子細胞生物学国際シンポジウム
  • Place of Presentation: くまもと県民交流館パレア(熊本)
• [Presentation] Absence of "natural" IgM-secreting plasma cells in Btk^<xid> bone marrow (3-E-W51-2-P). (2009)
  • Author(s): Wagatsuma, K., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2009-12-04
• [Presentation] A Notch ligand, Delta-like 1 functions as an adhesion molecule (1-I-W16-15-P). (2009)
  • Author(s): Murata, A., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2009-12-02
• [Presentation] CCR7非依存的に皮膚自己抗原を輸送する細胞の同定/Detection of dermal cells transporting skin antigens in CCR7-independent manner under steady state conditions (1-B-W2-9-P). (2009)
  • Author(s): Yoshino, M., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2009-12-02
• [Presentation] B220^+ Cd19^+ Flt3^+ Il7rα^+ bone-marrow precursors give rise to B-lineage cells in the presence of a Notch ligand, Delta-like 1 (1-E-W7-6-0/P). (2009)
  • Author(s): Okuyama, K., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: グランキューブ大阪(大阪)
  • Year and Date: 2009-12-02
• [Presentation] A Notch ligand, Delta-like-1 functions as an adhesion molecule for mast cells. Immune Regulation : Present and Future. (2009)
  • Author(s): Murata, A., Okuyama, K., Sakano, S., Yagita, H., Miyake, K., Wagatsuma, K., Moriyama, T., Yoshino, M., Hayashi, S.I.
  • Organizer: The First International Kishimoto Foundation Symposium.
  • Place of Presentation: 大阪
• [Presentation] A Notch ligand, Delta-like 1 functions as an adhesion molecule. (2009)
  • Author(s): Murata, A., Okuyama, K., Wagatsuma, K., Moriyama, T., Sakano, S., Yagita, H., Miyake, K., Akashi-Takamura, S., Yoshino, M., Hayashi S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
• [Presentation] CCR7非依存的に皮膚自己抗原を輸送する細胞の同定/Detection of dermal cells transporting skin antigens in CCR7-independent manner under steady state conditions. (2009)
  • Author(s): Yoshino, M., Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Hayashi S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
• [Presentation] B220^+ Cd19^+ Flt3^+ Il7rα^+ bone-marrow precursors give rise to B-lineage cells in the presence of a Notch ligand, Delta-like 1. (2009)
  • Author(s): Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Yoshino, M., Hayashi, S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
• [Presentation] Absence of "natural" IgM-secreting plasma cells in Btk^<xid> bone marrow. (2009)
  • Author(s): Wagatsuma, K., Okuyama, K., Murata, A., Moriyama, T., Yoshino, M., Hayashi, S.I.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
• [Presentation] A Notch ligand, Delta-like-1 functions as an adhesion molecule for mast cells. (2009)
  • Author(s): Murata, A., et al.
  • Organizer: The First International Kishimoto Foundation Symposium
  • Place of Presentation: グランキューブ大阪(大阪)
• [Presentation] A novel function of a Notch ligand, Delta-like-l as adhesion molecule for mast cells (3-G-W46-15-P). (2008)
  • Author(s): Murata, A., et al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] CCR7非依存的な皮膚自己抗原のリンパ節への輸送/CCR7-independent trafficking of skin antigens to regional lymph nodes (2-A-W17-2-O/P). (2008)
  • Author(s): Yoshino, M., et al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Flt3^- IL-7Rα^- B220^- BM precursors given rise to B-lineage cells in the presence of a Notch ligand, Delta-like-1 (2-C-W21-8-O/P). (2008)
  • Author(s): Okuyama, K., et al.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Effect of CCR7 on steady-state trafficking of skin dendritic cells to regional lymph nodes. (2008)
  • Author(s): Yoshino, M., Osaka, M., Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Hayashi, S.I.
  • Organizer: The 10th International Symposium on Dendritic Cells.
  • Place of Presentation: 神戸
• [Presentation] CCR7非依存的な皮膚自己抗原のリンパ節への輸送/CCR7-independent trafficking of skin antigens to regional lymph nodes. (2008)
  • Author(s): Yoshino, M., Osaka, M., Okuyama, K., Murata, A., Moriyama, T., Wagatsuma, K., Hayashi, S.I.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
• [Presentation] Flt3^+ IL-7R・^+ B220^+ BM precursors given rise to B-lineage cells in the presence of a Notch ligand, Delta-like-1. (2008)
  • Author(s): Okuyama, K., Murata, A., Osaka, M., Moriyama, T., Wagatsuma, K., Yoshino, M., Hayashi, S.I.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
• [Presentation] A novel function of a Notch ligand, Delta-like-1 as adhesion molecule for mast cells. (2008)
  • Author(s): Murata, A., Okuyama, K., Osaka, M., Moriyama, T., Wagatsuma, K., Sakano, S., Yoshino, M., Hayashi, S.I.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
• [Presentation] Effect of CCR7 on steady-state trafficking of skin dendritic cells to regional lymph nodes. (2008)
  • Author(s): Yoshino, M., et al.
  • Organizer: The 10th International Symposium on Dendritic Cells
  • Place of Presentation: 神戸
• [Book] A Humana Press Book(Methods for investigation of osteoclastogenesis using mouse embryonic stem cells. In"Embryonic Stem Cell Therapy for Osteo-Degenerative Diseases". (Methods in Molecular Biology, Vol.690))(Ed.N.I.zur Nieden) (2011)
  • Author(s): Tsuneto, M., Yamane, T., Hayashi, S.I.
  • Publisher: Springer Science+Business Media
• [Book] Embryonic stem cell therapy for osteo-degenerative diseases (Methods in Molecular Biology, Vol.690) (2011)
  • Author(s): Tsuneto, M., et al.
  • Total Pages: 336
  • Publisher: A Humana Press Book, Springer Science+Business Media
• [Book] Embryonic stem cell therapy for osteodegenerative diseases
  • Author(s): Tsuneto, M., Yamane, T., Hayashi, S.I.
  • Publisher: Humana Press Inc., Totowa, NJ.USA(印刷中)
• [Book] Methods for investigation of osteoclastogenesis using mouse embryonic stem cells. In "Embryonic Stem Cell Therapy for Osteodegenerative Diseases". (Methods in Molecular Biology), Ed. N. I. zur Nieden.
  • Author(s): Tsuneto, M., et al.
  • Publisher: Humana Press Inc., Totowa, NJ. USA (In press)
• [Remarks] ホームページ
  • URL: http://www.med.tottori-u.ac.jp/smgkjm/229/2201.html
• [Remarks]
  • URL: http://www.med.tottori-u.ac.jp/immunol/
• [Remarks]
  • URL: http://www.med.tottori-u.ac.jp/p/igaku/gakka/seimei/bunshi/meneki_home/
• [Remarks]
  • URL: http://www.med.tottori-u.ac.jp/p/igaku/gakka/seimei/bunshi/meneki_home/