Identification of downstream regulatory factor in RET signaling pathway and its application to cancer treatment
| Project/Area Number |
20590412
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | Chubu University |
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Principal Investigator |
ICHIHARA Masatoshi Chubu University, 生命健康科学部, 教授 (00314013)
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| Co-Investigator(Kenkyū-buntansha) |
IWAMOTO Takashi 中部大学, 生命健康科学部, 教授 (60223426)
QIAO Shanlou 中部大学, 生命健康科学部, 准教授 (00343658)
MURAKUMO Yoshiki 名古屋大学, 大学院・医学系研究科, 准教授 (40324438)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | RET / チロシンキナーゼ / アイソフォーム / DNAマイクロアレイ / isoform / LC/MS / LC / MS |
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| Research Abstract |
Ret tyrosine kinase (RET) plays critical roles in embryonic development as well as oncogenesis. RET is mainly expressed as two isoforms, RET51 and RET9, which have different C-terminal ends generated through alternative splicing , whereas previous studies show that RET9 is indispensable for nephrogenesis and enteric neurogenesis. We found remarkable increase of tyrosine- phosphorylated proteins in the cells after inducible expression of RET9 in MEF3T3 cells. We demonstrated that 261 genes were differentially expressed under inducible expression of either RET51 or RET9 by gene expression profiling. Ingenuity pathway analysis revealed that gene expression-related genes, including transcription factors, were enriched in these differentially expressed genes. Next we tried to identify differentially binding protein to RET between RET51 and RET9 using LC/MS, but we failed to find such candidate proteins, accounting for different function between RET51 and RET9.
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Report
(4 results)
Research Products
(98 results)
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[Journal Article] ATRA inhibits ceramide kinase transcription in a human neuroblastoma cell line, SH-SY5Y cells : the role of COUP-TFI.2010
Author(s)
Murakami M, Ito H, Hagiwara K, Yoshida K, Sobue S, Ichihara M, Takagi A, Kojima T, Tanaka K, Tamiya-Koizumi K, Kyogashima M, Suzuki M, Banno Y, Nozawa Y, Murate T
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Journal Title
J Neurochem. 112
Pages: 511-520
Related Report
Peer Reviewed
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[Journal Article] REGgamma modulates p53 activity by regulating its cellular localization.2010
Author(s)
Liu J, Yu G, Zhao Y, Zhao D, Wang Y, Wang L, Li L, Zeng Y, Dang Y, Wang C, Gao G, Long W, Lonard DM, Qiao S, Tsai MJ, Zhang B, Luo H, Li X
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Journal Title
J Cell Sci. 123
Pages: 4076-4084
Related Report
Peer Reviewed
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[Journal Article] Analysis of DOK-6 function in downstream signaling of RET in human neuroblastoma cells.2010
Author(s)
Kurotsuchi A, Murakumo Y, Jijiwa M, Kurokawa K, Itoh Y, Kodama Y, Kato T, Enomoto A, Asai N, Terasaki H, Takahashi M
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Journal Title
Cancer Sci. 101
Pages: 1147-1155
NAID
Related Report
Peer Reviewed
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[Journal Article] Molecular hydrogen is protective against 6-hydroxydopamine-induced nigrostriatal degeneration in a rat model of Parkinson's disease.2009
Author(s)
Fu Y, Ito M, Fujita Y, Ichihara M, Masuda A, Suzuki Y, Maesawa S, Kajita Y, Hirayama M, Ohsawa I, Ohta S, Ohno K
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Journal Title
Neurosci Lett. 453
Pages: 81-85
Related Report
Peer Reviewed
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[Journal Article] Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus.2009
Author(s)
Enomoto A, Asai N, Namba T, Wang Y, Kato T, Tanaka M, Tatsumi H, Taya S, Tsuboi D, Kuroda K, Kaneko N, Sawamoto K, Miyamoto R, Jijiwa M, Murakumo Y, Sokabe M, Seki T, Kaibuchi K, Takahashi M
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Journal Title
Neuron. 63
Pages: 774-787
Related Report
Peer Reviewed
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[Journal Article] A novel GDNF-inducible gene, BMZF3, encodes a transcriptional repressor associated with KAP-1.2008
Author(s)
Suzuki C, Murakumo Y, Kawase Y, Sato T, Morinaga T, Fukuda N, Enomoto A, Ichihara M, Takahashi M
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Journal Title
Biochem Biophys Res Commun. 366
Pages: 226-232
Related Report
Peer Reviewed
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[Journal Article] Roles of induced expression of MAPK phosphatase-2 in tumor development in RET-MEN2A transgenic mice.2008
Author(s)
Hasegawa T, Enomoto A, Kato T, Kawai K, Miyamoto R, Jijiwa M, Ichihara M, Ishida M, Asai N, Murakumo Y, Ohara K, Niwa Y, Goto H, Takahashi M
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Journal Title
Oncogene. 27
Pages: 5684-5695
Related Report
Peer Reviewed
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