Analysis of mechanisms involved in multifunction of tumor invasion factor emmprin

• Project/Area Number: 20590415
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Experimental pathology
• Research Institution: Fukuoka University
• Principal Investigator: NABESHIMA Kazuki Fukuoka University, 医学部, 教授 (40189189)
• Co-Investigator(Kenkyū-buntansha): HAMASAKI Makoto 福岡大学, 医学部, 講師 (90412600) ; AOKI Mikiko 福岡大学, 医学部, 助教 (80469379)
• Co-Investigator(Renkei-kenkyūsha): KOSHIKAWA Naohiko 東京大学, 医科学研究所, 助教 (70334282)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 腫瘍 / マトリックス・メタロプロテアーゼ / 浸潤 / 転移

Research Abstract

To analyze the mechanisms that are responsible for multifunction of emmprin, a tumor invasion-promoting factor, we induced crosslink among cell surface molecules by using a crosslinker BS3. The molecules, which had been crosskinked to emmprin, were analyzed by means of mass spectrometer. Up to now, the analysis has narrowed down to about 10 candidate molecules. Their abilities to complex with emmprin and stimulate fibroblasts to produce MMPs are now under investigation.

Research Products

• [Journal Article] Synthetic emmprin peptides inhibit tumor cell-fibroblast interaction-stimulated upregulation of MMP-2 and tumor cell invasion
  • Author(s): Koga K, Aoki M, SameshimaT, Hamasaki M, Egawa N, Seiki M, Toole BP, Suzumiya J, Nabeshima K
  • Journal Title: Int J Oncol, accepted
  • Peer Reviewed
• [Journal Article] Synthetic emmprin peptides with chitobiose substitution stimulate MMP-2 production by fibroblasts
  • Author(s): Kawakami T, Sameshima T, Hojo H, Koga K, Nakahara Y, Toole BP, Suzumiya J, Okada Y, Iwasaki A, Nabeshima K
  • Journal Title: BMC Cancer, under revision
  • Peer Reviewed
• [Presentation] Emmprin is released as glycocalyceal bodies (2009)
  • Author(s): Aoki M, Nabeshima K, et al.
  • Organizer: 8th Pan-Pacific Connective Tissue Societies Symposium
  • Place of Presentation: Shonan Village Center
• [Presentation] Emmprin is released as glycocalyceal bodies (2009)
  • Author(s): Aoki M, Nabeshima K et al
  • Organizer: 8th Pan-Pacific Connective Tissue Societies Symposium.
  • Place of Presentation: Shonan Village Center
• [Remarks] ホームページ等