| Project/Area Number |
20590418
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Experimental pathology
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
SUZUKI Yuko National Center of Neurology and Psychiatry, 神経研究所・遺伝子疾患治療研究部, 室長 (00342931)
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| Co-Investigator(Kenkyū-buntansha) |
TAKEDA Shin'ichi 独)国立精神・神経医療研究センター, 神経研究所・遺伝子疾患治療研究部, 部長 (90171644)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 骨格筋 / 筋再生 / 筋ジストロフィー / 細胞移植 / 幹細胞 / 筋芽細胞 / Side Population細胞 / CD248 / 細胞老化 / 筋衛星細胞 / SP細胞 / 骨格筋間葉系細胞 |
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| Research Abstract |
We found that CD31(-) CD45(-) side population (SP) cells in skeletal muscle are activated during muscle regeneration and promote the proliferation and the migration of grafted myoblasts. To further clarify the roles for CD31(-) CD45(-) SP cells, we generated CD248-sDTR-EGFP transgenic mice. However, the mice did not express sDTR-EGFP. This could be because the 1.0 kb upstream sequences of the CD248 gene were not enough to drive the expression of the reporter gene. We next analyzed the genome-wide gene expression pattern of mesenchymal cells (a descendent of CD31(-) CD45(-) SP cells) from dystrophin-deficient mice of different age. We found that mesenchymal cells in mdx mice experience cell senescence and immune response- and defense-related genes. These findings suggest that impaired muscle regeneration seen in advanced dystrophic muscle can be partly explained by exhausted mesenchymal cells, which otherwise promote muscle regeneration.
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