Regulation of IL12 production in the induction of protective immune response against Leishmania infection

• Project/Area Number: 20590424
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: HONMA Kiri Nagasaki University, 大学院・医歯薬学総合研究科, 講師 (70307940)
• Co-Investigator(Kenkyū-buntansha): YUI Katsuyuki 長崎大学, 大学院・医歯薬学総合研究科, 教授 (90274638)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: リーシュマニア / IRF4 / マクロファージ / 樹状細胞 / 糖鎖 / IL12 / IRF-4 / Th1

Research Abstract

Reduction of footpad swelling in IRF4 KO after Leishmania infection compared to resistant mice revealed to be due to defect of IRF4 in dendritic cells not macrophages by the experiments using conditional KO. Unlike splenic DC, CD4+ DC subset was observed in lymph node in IRF4 KO. It suggested that DC differentiation in LN was different from that in spleen. Recruitment of CD103+ DC, which was a kind of skin DC, in IRF4 KO was slower than WT within 48 hrs after infection.

Research Products

• [Journal Article] Characterization of waves of leukocyte recruitement to the lung allograft and the effect of CTLA4-Ig (2011)
  • Author(s): Taguchi.T., et.al.
  • Journal Title: Acta Medica Nagasakiensia Volume: (In press)
  • Peer Reviewed
• [Journal Article] The Jmjd3-Irf4 axis regulates M2 macrophage polarization and host responses against helminth infection. (2010)
  • Author(s): Satoh T., Takeuchi O., Vandenbon A., Yasuda K., Tanaka Y., Kumagai Y., Miyake I., Matsushita K., Okazaki T., Saitoh T., Honma K., Matsuyama T., Yui K., Tsujimura T., Standley D., Nakanishi K., Nakai K., Akira S.
  • Journal Title: Nat.Immunol. 11 Pages: 936-944
• [Journal Article] Production of IFN-γ by CD4(+) T cells in response to malaria antigens is IL-2 dependent. (2010)
  • Author(s): Kimura D., et.al.
  • Journal Title: International Immunology Volume: 22 Pages: 941-952
  • Peer Reviewed
• [Journal Article] The Jmjd3-irf4 axis regulates M2 macrophage polarization and host responses against helminth infection. (2010)
  • Author(s): Satoh T., et.al.
  • Journal Title: Nature Immunology Volume: 11 Pages: 936-944
  • Peer Reviewed
• [Journal Article] Th1分化に関わる転写因子 (2009)
  • Author(s): 本間季里、由井克之
  • Journal Title: 臨床免疫・アレルギー科 51 Pages: 456-461
• [Journal Article] Th1分化に関わる転写因子 (2009)
  • Author(s): 本間季里、由井克之
  • Journal Title: 臨床免疫・アレルギー科 51巻 Pages: 456-461
• [Journal Article] Interferon regulatory factor-4 differentially regulates the production of Th2 cytokines in naive vs.effector/memory CD4^+ T cells. (2008)
  • Author(s): Honma K., Kimura D., Tominaga T., Miyakoda M., Matsuyama T., Yui K.
  • Journal Title: Proc Natl Acad Sci USA 105 Pages: 15890-15895
• [Journal Article] Malaria-specific and non-specific activation of CD8+ T-cells during infection with Plasmodium berghei. (2008)
  • Author(s): M.Miyakoda, D.Kimura, M.Yuda, Y.Chinzei, Y.Shibata, Honma K., Yui K.
  • Journal Title: J.Immunol. 181 Pages: 1420-1428
• [Journal Article] Malaria-specific and nonspecific activation of CD8^+ T cells during blood stage of Plasmodium berghei infection (2008)
  • Author(s): Mana Miyakoda
  • Journal Title: Journal of Immunology 181 Pages: 1420-1428
  • Peer Reviewed
• [Journal Article] Interferon regulatory factor 4 differentially regulates the production of Th2 cytokines in naive vs. effector/memory CD4^+T cells. (2008)
  • Author(s): Kiri Honma
  • Journal Title: Proc Nat Acad Sci USA 105 Pages: 15890-15895
  • NAID: 120006981735
  • Peer Reviewed
• [Journal Article] Production of IFN-γ by CD4+ T cells in response to malaria antigens is IL-2-dependent.
  • Author(s): Kimura D., Miyakoda M., Honma K., Shibata Y., Yuda M., Chinzei Y., Yui K.
  • Journal Title: Int.Immunol. In press
• [Journal Article] Characterization of waves of leukocyte recruitment to the lung allograft and the effect of CTLA4-Ig.
  • Author(s): T.Taguchi, Y.Inamura, Honma K., D.Kimura, M.Miyakoda, T.Tagawa, N.Yamasaki, T.Tsuchiya, T.Nagayasu, Yui K.
  • Journal Title: Acta Medica Nagasakiensia. In press.
  • NAID: 110008767588
• [Presentation] Identification of the IRF-4 domain that is critical for Th2 differentiation of CD4^+ T cells. (2010)
  • Author(s): 本間季里
  • Organizer: 第14回国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] Identification of the IRF-4 domain that is critical for Th2 differentiation of CD4^+ T cells (2010)
  • Author(s): Honma K.
  • Organizer: The 14 th International congress of Immunology
  • Place of Presentation: Kobe, Japan
  • Year and Date: 2010-08-23
• [Presentation] Th2非依存的なN.brasiliensis感染排除機構の解析 (2010)
  • Author(s): 本間季里
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 旭川
  • Year and Date: 2010-05-20
• [Presentation] The role of IRF-4 expressed in dendritic cells in the immune responses against Leishmania major. (2010)
  • Author(s): Masoud akibari
  • Organizer: 第79回日本寄生虫学会
  • Place of Presentation: 旭川
  • Year and Date: 2010-05-20
• [Presentation] Th2 independent N.brasiliensis expulsion. (2010)
  • Author(s): 本間季里
  • Organizer: the 10th Nagasaki-ssingapore medical symposium
  • Place of Presentation: シンガポール
  • Year and Date: 2010-04-15
• [Presentation] Th2 independent N.brasiliensis expulsion (2010)
  • Author(s): Honma K.
  • Organizer: 10^<th> Nagasaki-Singapore Medical Symposium on Infectious Diseases
  • Place of Presentation: Singapore
  • Year and Date: 2010-04-15
• [Presentation] Th2非依存的なN.brasiliensis排除機構の解析 (2010)
  • Author(s): 本間季里
  • Organizer: 第8回感染症沖縄フォーラム
  • Place of Presentation: 健康文化村カルチャーリゾートフェストーネ
  • Year and Date: 2010-02-13
• [Presentation] マラリア感染における記憶CD8^+T細胞の反応解析 (2010)
  • Author(s): 都田真奈
  • Organizer: 第8回感染症沖縄フォーラム
  • Place of Presentation: 健康文化村カルチャーリゾートフェストーネ
  • Year and Date: 2010-02-12
• [Presentation] マラリア原虫感染における抗原特異的CD4^+T細胞のIFN-γ産生はIL-2依存的である (2010)
  • Author(s): 木村大輔
  • Organizer: 第8回感染症沖縄フォーラム
  • Place of Presentation: 健康文化村カルチャーリゾートフェストーネ
  • Year and Date: 2010-02-11
• [Presentation] IRF-4欠損CD4 T細胞においては、GATA-3発現 だけではTh2分化に不十分である (2009)
  • Author(s): 本間季里
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] IRF-4欠損CD4 T細胞においてはGATA-3発現はTh2分化に不十分である。 (2009)
  • Author(s): 本間季里
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-03
• [Presentation] マラリア感染における記憶・細胞の誘導と維持に関する解析 (2009)
  • Author(s): 都田真奈
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] マラリア原虫感染マウスのCD4+ T細胞は、抗原特異的T細胞が産生する液性因子によりIFN-γ産生を増幅する。 (2009)
  • Author(s): 木村大輔
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] Leishmania major感染初期の防御免疫におけるマクロファージ/樹状細胞に発現するIRF-4の役割 (2009)
  • Author(s): 本間季里
  • Organizer: 第78回日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] Leishmania major感染初期の防御免疫におけるマクロファージ/樹状細胞に発現するIRF-4の役割 (2009)
  • Author(s): 本間季里
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-28
• [Presentation] Differentia1 roles of interteron regulatory factor-4 (IRF-4)in the production of Th2 cytokines in naive vs. effector/memory CD4 T cells (2008)
  • Author(s): 本間季里
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-01
• [Presentation] Regulation of the immune system by interferon regulatory factor-4 (IRF-4)-The role of IRF4 in T cells and macrophages using the mouse infection models (2008)
  • Author(s): 本間季里
  • Organizer: The 9^<th> Nagasaki-Singapore Medical Symposium
  • Place of Presentation: 長崎
  • Year and Date: 2008-10-11
• [Presentation] Nippostrongyrus brasiliensis感染排除における転写因子IRF-4の重要性 (2008)
  • Author(s): 本間季里
  • Organizer: 日本寄生虫学会
  • Place of Presentation: 長崎
  • Year and Date: 2008-04-04
• [Presentation] Differential roles of interferon regulatory factor-4 (IRF-4) in the production of Th2 cytokines in naive vs.effector/memory CD4 T cells (2008)
  • Author(s): 本間季里
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
• [Presentation] Nippostrongyrus brasiliensis感染排除における転写因子IRF-4の重要性 (2008)
  • Author(s): 本間季里
  • Organizer: 第77回日本寄生虫学会
  • Place of Presentation: 長崎
• [Remarks] ホームページ等
• [Remarks] 長崎大学学術研究成果リポジトリ
  • URL: http://www.med.nagasaki-u.ac.jp/mmi/im/index.html
• [Remarks]
  • URL: http://naosite.lb.nagasaki-u.ac.jp/