Detection of TNF-α release from mast cells stimulated with Toxoplasma gondii.
Project/Area Number |
20590430
|
Research Category |
Grant-in-Aid for Scientific Research (C)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Parasitology (including Sanitary zoology)
|
Research Institution | Teikyo University |
Principal Investigator |
UEDA Takane Teikyo University, 医学部, 助教 (80459312)
|
Co-Investigator(Kenkyū-buntansha) |
ONO Yasuo 帝京大学, 医学部, 教授 (10177272)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
|
Keywords | 免疫 / 原虫 / BMMC / TNF-α / マウス好中球 / マスト細胞 / TNF / 感染症 / 免疫学 / 微生物 |
Research Abstract |
Bone marrow derived mast cells (BMMCs) from mice were examined for TNF-α release by stimulating with Toxoplasma gondii tachyzoites. BMMC stimulated with live tachyzoites of T.g (RH) or crude extracts released TNF-α in culture supernatant. TNF-α gene expression was also enhanced after T.g stimuli. Activation of neutrophils were observed in the cells stimulated with BMMC primed with T.g compared to stimulation with BMMC.
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Report
(4 results)
Research Products
(5 results)