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Analyses and applications of novel mechanism of phospho-independent regulation of lipid metabolism by Trypanosoma brucei 14-3-3

Research Project

Project/Area Number 20590434
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Parasitology (including Sanitary zoology)
Research InstitutionKurume University

Principal Investigator

INOUE Masahiro  Kurume University, 医学部, 教授 (00232562)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords脂質代謝 / 蛋白リン酸化酵素 / 結合蛋白 / ブルーストリパノソーマ / 2014 / 03 / 14-3-3タンパク / トリパノソーマ / 遊離脂肪酸 / トリグリセライド / リン酸化ペプチド / タンパク-タンパク相互作用 / 14-3-3 / 解糖系 / アフリカトリパノソーマ / Tb14-3-3 / Triglyceride / Free fatty acid / acetyl-CoA carboxylase / biotin-acetyl-CoA ligase / acetyl-CoA synthetase / Tb14-3-3ノックダウン昆虫型細胞株
Research Abstract

Trypanosoma brucei is a causative agent of sleeping sickness. More than 30,000 infected patients died of this devastating disease. One of the aims for this research is to identify the molecular mechanisms by which T. brucei 14-3-3s control the motility, cytokinesis, and cell cycle focusing on the lipid metabolism controlled by Tb14-3-3s as we discovered. We found that Tb14-3-3s interact with the enzymes involved in sugar or lipid metabolism, and protein kinases. One of the protein kinases among them is found out to be critical for the regulation of the motility, cytokinesis and cell cycle.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (18 results)

All 2010 2009 2008

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (6 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Phosphorylation- Dependent Protein Interaction with Trypanosoma brucei 14-3-3 Proteins that Display Atypical Target Recognition.2010

    • Author(s)
      Inoue M., et al.
    • Journal Title

      PLoS One 5(12)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Epistatic and Functional Interactions of Catecho-O-Methyltransferase(COMT) and AKT1 on Neuregulin 1-ErbB Signaling in Cell Models.2010

    • Author(s)
      Sei Y., et al.
    • Journal Title

      PLoS One 5(5)

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation-Dependent Protein Interaction with Trypanosoma brucei 14-3-3 Proteins that Display Atypical Target Recognition.2010

    • Author(s)
      M.Inoue, et al.
    • Journal Title

      PLoS One

      Volume: 5(12)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Epistatic and Functional Interactions of Catecho-O-Methyltransferase(CO MT) and AKT1 on Neuregulin 1-ErbB Signaling in Cell Models.2010

    • Author(s)
      Yoshitatsu Sei, et al.
    • Journal Title

      PLoS One

      Volume: 5(5)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Critical Role for highly Conserved GLU610 Residue of Oligopeptidase B from Trypanosoma Brucei in Thermal Stability2010

    • Author(s)
      Nor Ismaliza Mohd Ismail, et al.
    • Journal Title

      The Journal of Biochemistry 147

      Pages: 201-211

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation-dependent Lys63-linked polyubiquitination of Daxx is essential for sustained TNF -{alpha}-induced ASK1 activation.2009

    • Author(s)
      Fukuyo Y., Kitamura T., Inoue M., Horikoshi NT, Higashikubo R, Hunt CR, Usheva A, Horikoshi N.
    • Journal Title

      Cancer Res. 69(19)

      Pages: 7512-7517

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutant p53 disrupts the stress MAPK activation circuit induced by ASK1-dependent stabilization of Daxx.2009

    • Author(s)
      Kitamura T., Fukuyo Y., Inoue M., Horikoshi NT., Shindoh, M., Rogers BE., Usheva, A., Horikoshi, N.
    • Journal Title

      Cancer Res. 69(19)

      Pages: 7681-7688

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Critical Role for highly Conserved GLU610 Residue of Oligopeptidase B from Trypanosoma Brucei in Thermal Stability.2009

    • Author(s)
      Ismail NI., Yuasa T., Yuasa K., Nambu Y, Nisimoto M., Goto M., Matsuki H., Inoue M., Nagahama M., Tsuji A.
    • Journal Title

      J.Biochem. 147(2)

      Pages: 201-211

    • NAID

      10027873746

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mutant p53 disrupts the stress MAPK activation circuit induced by ASK1-denendent stabilization of Daxx2009

    • Author(s)
      Tetsuya Kitamura, et al.
    • Journal Title

      Cancer Research 69

      Pages: 7681-7688

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphorylation-dependent Lys63-linked polyubiquitination of Daxx is essential for sustained TNF-α-induced ASK1 activation2009

    • Author(s)
      Yayoi Fukuyo, et al.
    • Journal Title

      Cancer Research 69

      Pages: 7512-7517

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Oxidative Stress Plays a Criticaal Role in Inactivating Mutant BRAF by Geldanamycin Derivatives.2008

    • Author(s)
      Y. Fukuyo, M. Inoue, T. Nakajima, et al.
    • Journal Title

      Cancer Res 68

      Pages: 6324-6330

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] 普遍的でない分子認識機構をもつトリパノソーマ14-3-3に結合するキナーゼの発見2010

    • Author(s)
      井上雅広
    • Organizer
      第9回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      長崎大学坂本キャンパス(長崎市)
    • Year and Date
      2010-10-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] Biological significance of heterodimeric form of Trypanosoma brucei 14-3-3I and II.2009

    • Author(s)
      井上雅広、安田幸一、上村春樹、杉本佳織、井上大志、福間利英
    • Organizer
      第78回日本寄生虫学会大会
    • Place of Presentation
      東京
    • Year and Date
      2009-03-27
    • Related Report
      2010 Final Research Report
  • [Presentation] Biological significance of heterodimeric form of Trypanosoma brucei 14-3-3 I and II2009

    • Author(s)
      井上雅広, 他
    • Organizer
      第78回日本寄生虫学会大会
    • Place of Presentation
      法政大学(東京)
    • Year and Date
      2009-03-27
    • Related Report
      2008 Annual Research Report
  • [Presentation] Biological significance of heterodimeric form of Trypanosoma brucei 14-3-3I and II.2009

    • Author(s)
      井上雅広
    • Organizer
      日米医学協力研究会 寄生虫疾患専門部会 第43回日米合同会議
    • Place of Presentation
      東京
    • Year and Date
      2009-01-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Biological significance of heterodimeric form of Trypanosoma brucei 14-3-3 I and II2009

    • Author(s)
      Masahiro Inoue
    • Organizer
      日米医学協力研究会 寄生虫疾患専門部会第43回日米合同会議
    • Place of Presentation
      国立医療センター(東京)
    • Year and Date
      2009-01-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] 普遍的でない分子認識機構をもつトリパノソーマ14-3-3 に結合するキナーゼの発見2008

    • Author(s)
      井上雅広
    • Organizer
      第9回分子寄生虫・マラリア研究フォーラム
    • Place of Presentation
      長崎
    • Year and Date
      2008-10-09
    • Related Report
      2010 Final Research Report
  • [Patent(Industrial Property Rights)] 原虫の増殖阻害剤を検出する方法2008

    • Inventor(s)
      井上雅広
    • Industrial Property Rights Holder
      井上雅広、上村春樹
    • Filing Date
      2008-10-08
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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