Molecular Analysis of Life and Death in Pathogenic Fungi Cryptococcus neoformans : Cell Cycle Cntrol and Interaction between Fungi and Bacteria

• Project/Area Number: 20590437
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Chiba University
• Principal Investigator: KAWAMOTO Susumu Chiba University, 真菌医学研究センター, 教授 (80125921)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 真菌 / 感染症 / 細胞周期 / 遺伝子 / タンパク質 / 国際情報交換 / チェコ:ハンガリー / 蛋白質

Research Abstract

The G1 Cyclin CnCln1 gene, a key molecule in the cell cycle control in a pathogenic yeast, Cryptococcus neoformans, was cloned, identified and characterized. Interaction between cell cycle control and stress (hypoxia) response was analyzed. Also, localization of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus was demonstrated by scanning immunoelectron microscopy.

Research Products

• [Journal Article] The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression but has important roles in the proper commitment to DNA synthesis and bud emergence in this yeast. (2010)
  • Author(s): Virutudazo EV, Kawamoto S, Ohkusu M, Aoki S, Sipiczki M, Takeo K
  • Journal Title: FEMS Yeast Research 10(5) Pages: 605-618
  • Peer Reviewed
• [Journal Article] Localization by scanning immunoelectron microscopy of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus. (2010)
  • Author(s): Yamaguchi M, Ikeda R, Nishimura M, Kawamoto S
  • Journal Title: Microbiol Immunol 54 Pages: 368-370
  • NAID: 10029545273
  • Peer Reviewed
• [Journal Article] Deletion of CnLIG4 DNA ligase gene in the fungal pathogen Cryptococcus neoformans elevates homologous recombination efficiency. (2010)
  • Author(s): Shimizu K, Li H-M, Virtudazo EV, Watanabe A, Kamei K, Yamaguchi M, Kawamoto S
  • Journal Title: Mycoscience 51 Pages: 28-33
  • NAID: 10027042936
  • Peer Reviewed
• [Journal Article] Non-homologous end joining pathway of the human pathogen Cryptococcus neoformans influences homologous integration efficiency but not virulence. (2010)
  • Author(s): Li H-M, Virtudazo EV, Toh-e A, Yamaguchi M, Kawamoto S, Shimizu K
  • Journal Title: Mycoscience 51 Pages: 272-280
  • NAID: 10026513911
  • Peer Reviewed
• [Journal Article] The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression but has important roles in the proper commitment to DNA synthesis and bud emergence in this yeast. (2010)
  • Author(s): Virtudazo, Eric
  • Journal Title: FEMS Yeast Research Volume: 10 Pages: 605-618
  • Peer Reviewed
• [Journal Article] Localization by scanning immunoelectron microscopy of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus. (2010)
  • Author(s): Yamaguchi, Masashi
  • Journal Title: Microbiology and Immunology Volume: 54 Pages: 368-370
  • Peer Reviewed
• [Journal Article] Deletion of CnLIG4 DNA ligase gene in the fungal pathogen Cryptococcus neoformans elevates homologous recombination efficiency. (2010)
  • Author(s): Shimizu, Kiminori
  • Journal Title: Mycoscience Volume: 51 Pages: 28-33
  • Peer Reviewed
• [Journal Article] Non-homologous end joining pathway of a human pathogen Cryptococcus neoformans influences homologous integration efficiency but not virulence. (2010)
  • Author(s): Li, Hao-Man
  • Journal Title: Mycoscience Volume: 51 Pages: 272-280
  • Peer Reviewed
• [Journal Article] Deletion of CnLIG4 DNA ligase gene in the fungal pathogen Cryptococcus neoformans elevates homologous recombination efficiency. (2010)
  • Author(s): Shimizu, Kiminori
  • Journal Title: Folia Microbiol 51 Pages: 28-33
  • Peer Reviewed
• [Journal Article] Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats. (2009)
  • Author(s): Raclavsky V, Husickova V, Moranova Z, Ohkusu M, Fischer O, Precek J, Trtkova J, Takeo K, Kawamoto S
  • Journal Title: Folia Microbiol 54(4) Pages: 349-352
  • Peer Reviewed
• [Journal Article] Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates. (2009)
  • Author(s): Raclavsky V, Pavlicek J, Novotny R, Moranova Z, Ohkusu M, Trtkova J, Takeo K, Kawamoto S
  • Journal Title: Folia Microbiol 54(4) Pages: 369-371
  • Peer Reviewed
• [Journal Article] Hypoxia sensing in Cryptococcus neoformans : Biofilm-like adaptation for dormancy? (2009)
  • Author(s): Moranova Z, Kawamoto S, Raclavsky V
  • Journal Title: Biomedical Papers 153(3) Pages: 189-193
  • Peer Reviewed
• [Journal Article] Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats. (2009)
  • Author(s): Raclavsky, Vladislav
  • Journal Title: Folia Microbiol 54 Pages: 349-352
  • Peer Reviewed
• [Journal Article] Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates. (2009)
  • Author(s): Raclavsky, Vladislav
  • Journal Title: Folia Microbiol 54 Pages: 369-371
  • Peer Reviewed
• [Journal Article] Hypoxia sensing in Cryptococcus neoformans : Biofilm-like adaptation for dormancy? (2009)
  • Author(s): Moranova, Zuzana
  • Journal Title: Biomed Pap Med 153 Pages: 189-193
  • Peer Reviewed
• [Journal Article] Cryptococcus neoformansの細胞周期制御におけるGIサイクリン遺伝子upstreamORFの役割 (2008)
  • Author(s): 大畑,美穂子
  • Journal Title: 日本医真菌学会雑誌 49 Pages: 102-102
  • Peer Reviewed
• [Journal Article] Cryptococcus neoformansのCdc25ホスファターゼホモログのクローニングと構造機能解析 (2008)
  • Author(s): ヴィルトウダゾ,エリック
  • Journal Title: 日本医真菌学会雑誌 49 Pages: 103-103
  • Peer Reviewed
• [Journal Article] Non-homologous end joining pathway of a human pathogen Cryptococcus neoformans influences homologous integration efficiency but not virulence.
  • Author(s): Li, Hao-Man
  • Journal Title: Mycoscience (印刷中)
  • Peer Reviewed
• [Journal Article] Isolation of yeasts from palm tissues damaged by the red palm weevil and their possible effect on the weevil overwintering
  • Author(s): Abe, Fukiko
  • Journal Title: Mycoscience (印刷中)
  • Peer Reviewed
• [Journal Article] Localization by scanning immunoelectron microscopy of triosephosphate isomerase, the molecules responsible for contact-mediated killing of Cryptococcus, on the surface of Staphylococcus.
  • Author(s): Yamaguchi, Masashi
  • Journal Title: Microbiology and Immunology (印刷中)
  • Peer Reviewed
• [Journal Article] The single Cdk1-G1 cyclin of Cryptococcus neoformans is not essential for cell cycle progression but has important roles in the proper commitment to DNA synthesis and bud emergence in this yeast.
  • Author(s): Virtudazo, Eric
  • Journal Title: FEMS Yeast Research (印刷中)
  • Peer Reviewed
• [Presentation] Two key players of cell cycle regulation in Cryptococcus neoformans, Cyclin-dependent kinase (Cdk) and its regulator (2010)
  • Author(s): 川本進
  • Organizer: Cyclin.
  • Place of Presentation: University of Wisconsin-Madison,Madison, Wisconsin, USA
  • Year and Date: 2010-11-22
• [Presentation] Two key players of cell cycle regulation in Cryptococcus neoformans, Cyclin-dependent kinase (Cdk) and its regulator、Cyclin. (2010)
  • Author(s): Kawamoto, Susumu
  • Organizer: Invited lecture at University of Wisconsin-Madison
  • Place of Presentation: Madison (USA)
  • Year and Date: 2010-11-22
• [Presentation] 真菌細菌の菌種間相互作用における電子顕微鏡的アプローチ (2010)
  • Author(s): 山口正視, 池田玲子, 川本進
  • Organizer: 第54回日本医真菌学会総会,真菌誌51(増刊1号):65
  • Place of Presentation: 東京
• [Presentation] アグロバクテリウム法によるCryptococcus neoformans形質転換に関する条件検討 (2010)
  • Author(s): 清水公徳, 太田裕子, 増田恵美, ヴィルトゥダゾ・エリック, 山口正視, 川本進
  • Organizer: 第54回日本医真菌学会総会,真菌誌51(増刊1号):65
  • Place of Presentation: 東京
• [Presentation] Cryptococcus neoformans Wee1 kinase is not essential for growth but has a role in G2-M. (2010)
  • Author(s): E.Virtudazo, M.Ohkusu, K.Shimizu, M.Yamaguchi, S.Kawamoto
  • Organizer: 第54回日本医真菌学会総会,真菌誌51(増刊1号):77
  • Place of Presentation: 東京
• [Presentation] Cell-cycle related genes in the pathogenic yeast Cryptococcus neoformans.9th International Mycological Congress (IMC9) (2010)
  • Author(s): Kawamoto S, Virtudazo EV, Ohkusu M, Takeo K
  • Organizer: The Biology of of Fungi
  • Place of Presentation: Edinburgh, UK
• [Presentation] The main cell cycle genes in the pathogenic yeast Cryptococcus neoformans. (2010)
  • Author(s): Kawamoto S, Virtudazo EV, Ohkusu M, Sonoda T, Miura S, Takeo K
  • Organizer: 10th European Conference on Fungal Genetics (ECFG10), Leeuwenhorst
  • Place of Presentation: The Netherlands
• [Presentation] The main cell cycle genes in the pathogenic yeast Cryptococcus neoformans. (2010)
  • Author(s): Kawamoto, Susumu
  • Organizer: 10^<th> European Conference on Fungal Genetics (ECFG10)
  • Place of Presentation: Leeuwenhorst(The Netherlands)
• [Presentation] Cell-cycle related genes in the pathogenic yeast Cryptococcus neoformans (2010)
  • Author(s): Kawamoto, Susumu
  • Organizer: 9^<th> International Mycological Congress (IMC9) : The Biology of Fungi
  • Place of Presentation: Edinburgh (UK)
• [Presentation] Cryptococcus neoformansWeel is not essential for growth but has a role in G2-Mcontrol. (2010)
  • Author(s): Virtudazo, Eric
  • Organizer: 第54回日本医真菌学会総会
  • Place of Presentation: 東京
• [Presentation] アグロバクテリウム法によるCryptococcus neoformans形質転換に関する条件検討 (2010)
  • Author(s): 清水、公徳
  • Organizer: 第54回日本医真菌学会総会
  • Place of Presentation: 東京
• [Presentation] Molecular analysis of cell cycle regulation in Cryptococcus neoformans. (2009)
  • Author(s): Kawamoto S, Virtudazo EV, Ohkusu M, Manova Z, Raclavsky V, Takeo K
  • Organizer: 4th Trends in Medical Mycology.
  • Place of Presentation: Athens,Greece
• [Presentation] Functional analysis of genes involved in drug resistance in Cryptococcus neoformans. (2009)
  • Author(s): Shimizu K, Shimizu M, Li H-M, Kawamoto S
  • Organizer: The 17th Congress of the International Society for Human and Animal Mycology (ISHAM 2009).Program and Abstract book p.280.
  • Place of Presentation: Tokyo, Japan
• [Presentation] Difference in physiology and protein expression pattern of capsule thickness variants of Cryptococcus neoformans. (2009)
  • Author(s): Ohkusu M, Kawamoto S
  • Organizer: ISHAM 2009.Program and Abstract p.355.
  • Place of Presentation: Tokyo, Japan
• [Presentation] Understanding cell control in the pathogenic yeast Cryptococcus neoformans. (2009)
  • Author(s): Virtudazo, E.V., Ohkusu, M., Ohata, M., Miklos, I., Sipiczki, Matthias, Aoki, S., Takeo, K., Kawamoto S.
  • Organizer: ISHAM 2009.
  • Place of Presentation: Tokyo, Japan.
• [Presentation] Analysis of the role of the single G1 cyclin, CnCln1, in Cryptococcus neoformans cell cycle. (2009)
  • Author(s): Virtudazo EV., Ohkusu M, Ohata M, Takeo K, Kawamoto S
  • Organizer: ISHAM 2009.
  • Place of Presentation: Tokyo, Japan.
• [Presentation] Pospisilova K, Raclavsky V : Study of hypoxia response in Cryptococcus neoformans. (2009)
  • Author(s): Moranova., Virtudazo EV, Ohkusu M, Kawamoto S
  • Organizer: ISHAM 2009.
  • Place of Presentation: Tokyo, Japan.
• [Presentation] The main cell cycle genes in the pathogenic yeasCryptococcus Neoformans. (2009)
  • Author(s): Kawamoto, Susumu
  • Organizer: 4th Trends in Medical Mycology
  • Place of Presentation: アテネ(ギリシャ)
• [Presentation] Understanding cell cycle control in the pathogenic yeast Cryptococcusneoformans. (2009)
  • Author(s): Virtudazo, Eric
  • Organizer: 17th Congress of the International Society for Human and Animal Mycology
  • Place of Presentation: 東京
• [Presentation] Analysis of the role of the single G1 cyclin, CnCln1, in Cryptococus neoformans cell cycle. (2009)
  • Author(s): Virtudazo, Eric
  • Organizer: 17th Congress of the International Society for Human and Animal Mycology
  • Place of Presentation: 東京
• [Presentation] Study of hypoxia response in Cryptococcus neoformans. (2009)
  • Author(s): Moranova, Zuzana
  • Organizer: 17th Congress of the International Society for Human and Animal Mycology
  • Place of Presentation: 東京
• [Presentation] Differences in physiology and protein expression pattern of capsule thickness variants of Cryptococcus neoformans. (2009)
  • Author(s): Ohkusu, Misako
  • Organizer: 17th Congress of the International Society for Human and Animal Mycology
  • Place of Presentation: 東京
• [Presentation] Functional analysis of gened involved in drug resistance in Cryptococcus neoformans. (2009)
  • Author(s): Shimizu, Kiminori
  • Organizer: 17th Congress of the International Society for Human and Animal Mycology
  • Place of Presentation: 東京
• [Presentation] 病原酵母クリプトコックスの細胞周期制御の分子的理解に向けて (2008)
  • Author(s): 川本進, E.Virtudazo, 大楠美佐子, 竹尾漢治, 田村裕, Z.Moranova, V.Raclavsky
  • Organizer: 第2回真菌ワープ研究会
  • Place of Presentation: 千葉
  • Year and Date: 2008-08-21
• [Presentation] Structural and functional analysis of the single G1 cyclin CnCln1 in Cryptococcus neoformans. (2008)
  • Author(s): Virtudazo EV, Ohkusu M, Ohata M, Miklos I, Sipiczki M, TakeoK, Kawamoto S
  • Organizer: 第91回日本細菌学会関東支部総会
  • Place of Presentation: 千葉市
• [Presentation] Towards molecular understanding of cell cycle regulation and cell signaling in Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto S, Virtudazo E, Ohata M, Ohkusu M, Shimizu K, Yamaguchi M, Miklos I, Sipiczki M, Moranova Z, Raclavsky V, Takeo K
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki
• [Presentation] Cloning and functional analysis of the Cryptococcus neoformans Cdc25/Mih1 phosphatase homologue. (2008)
  • Author(s): Virtudazo EV, Ohata M, Ohkusu M, Miklos I, Sipiczki M, Takeo K, Kawamoto S
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki, Japan.
• [Presentation] Analysis of the role of upstream ORFs in the regulation of CnCln1 expression in Cryptococcus neoformans. (2008)
  • Author(s): Ohata M., Virtudazo EV., Ohkusu M., Takeo K., Kawamoto S
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki, Japan.
• [Presentation] Study of hypoxia response in Cryptococcus neoformans. (2008)
  • Author(s): Moranova Z, Husiokova V, Ohkusu M, Kawamoto S, Raclavsky V
  • Organizer: 7th International Conference on Cryptococcus and Cryptococcosis.
  • Place of Presentation: Nagasaki
• [Presentation] Cryptococcus neoformansのCdc25ホスファターゼホモログのクローニングと構造機能解析 (2008)
  • Author(s): ヴィルトゥダゾ・エリック, 大畑美穂子, 大楠美佐子, Ida Miklos, Matthias Sipiczki, 竹尾漢治, 川本進
  • Organizer: 第52回日本医真菌学会総会
  • Place of Presentation: 長崎市
• [Presentation] Cryptococcus neoformansの細胞周期制御におけるG1サイクリン遺伝子upstreamORFの役割 (2008)
  • Author(s): 大畑美穂子, ヴィルトゥダゾ・エリック, 大楠美佐子, 竹尾漢治, 川本進
  • Organizer: 第52回日本医真菌学会総会
  • Place of Presentation: 長崎市
• [Presentation] Molecular approach to understanding cell regulation in a pathogenic yeast Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto S, Virtudazo E, Ohata M, Ohkusu M, Takeo K
  • Organizer: China-Japan Pan Asia Pacific Mycology Forum, Changchun 2008 Changchun
  • Place of Presentation: China
• [Presentation] Structural and functional analysis of cell cycle control genes in Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto S, Virtudazo E, Ohkusu M, Sonoda T, Miyagi Y, Okuda K, Takeo K
  • Organizer: 2008 Annual Meeting American Society for Biochemistry and Molecular Biology (Experimental Biology 2008)
  • Place of Presentation: San Diego, California, USA
• [Presentation] Structural and functional analysis of cell cycle control genes in Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto, Susumu
  • Organizer: 2008年米国生化学・分子生物学会年会
  • Place of Presentation: サンディエゴ(米国)
• [Presentation] Molecular approach to understanding cell regulation in a pathogenic yeast Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto, Susumu
  • Organizer: China-Japan Pan Asia Pacific Mycology Forum
  • Place of Presentation: 長春(中国)
• [Presentation] Towards molecular understanding of cell cycle regulation and cell signaling in Cryptococcus neoformans. (2008)
  • Author(s): Kawamoto, Susumu
  • Organizer: 第7回国際クリプトコックス会議
  • Place of Presentation: 長崎
• [Presentation] Cloning and functional analysis of the Cryptococcus neoformans Cdc25/Mihl phosphatase homologue. (2008)
  • Author(s): Virtudazo, Eric
  • Organizer: 第7回国際クリプトコックス会議
  • Place of Presentation: 長崎
• [Presentation] Analysis of the role of upstream ORFs in the regulation of CnClnl expression in Cryptococcus neoformans. (2008)
  • Author(s): Ohata, Mihoko
  • Organizer: 第7回国際クリプトコックス会議
  • Place of Presentation: 長崎
• [Book] 酵素利用技術大系～基礎・解析から改変・高機能化・産業利用まで(小宮山真監修)(酵素を創る-生物学的アプローチ-細胞を用いたタンパク質合成:昆虫培養細胞・バキュロウイルスベクター発現系を用いたタンパク質合成) (2010)
  • Author(s): 川本進
  • Publisher: エヌ・テイー・エス
• [Book] 酵素利用技術大系-基礎・解析から改変・高機能化・産業利用まで- (2010)
  • Author(s): 川本, 進
  • Publisher: エヌ・ティ・エス
• [Book] 酵素利用技術大系-基礎・解析から改変・高機能化・産業利用まで- (2010)
  • Author(s): 川本, 進
  • Total Pages: 1078
  • Publisher: エヌ・テイ・エス