Identification of low-molecular-mass secretory proteins of Mycobacterium tuberculosis and the application to development of anti-tuberculosis vaccines
| Project/Area Number |
20590438
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
NAGATA Toshi Hamamatsu University School of Medicine, 医学部, 教授 (90275024)
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| Co-Investigator(Kenkyū-buntansha) |
TSUJIMURA Kunio 浜松医科大学, 医学部, 准教授 (10227407)
KOIDE Yukio 浜松医科大学, 医学部, 理事 (30126809)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | DNAワクチン / 結核 / 低分子量分泌タンパク / T細胞エピトープ |
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| Research Abstract |
We identified here murine and human dominant T-cell epitopes of three low-molecular-mass secretory proteins, CFP11 (Rv2433c), CFP17 (Rv1827), TB18.5 (Rv0164) of Mycobacterium tuberculosis. Plasmid DNAs encoding these proteins were immunized to two inbred mice and HLA-DRB1^*0401 transgenic mice. T-cell epitopes were determined based on IFN-γ production from immune spleen cells. The results obtained here is feasible for analysis of the role of antigen-specific T cells during M.tuberculosis infection and the vaccine development.
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Report
(4 results)
Research Products
(49 results)
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[Journal Article] Identification of HLA-A*0201-restricted T-cell epitope on the MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis, by MPT51 overlapping peptide screening.2008
Author(s)
Aoshi T, Nagata T, Suzuki M, Uchijima M, Hashimoto D, Rafiei A, Suda T, Chida K, Koide Y
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Journal Title
Infect Immun 76
Pages: 1565-1571
Related Report
Peer Reviewed
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[Journal Article] Identification of an HL A-A^*0201-restricted T-cell epitope on the MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis, by MPT51 overlapping peptide screening.2008
Author(s)
Aoshi T, Nagata T, Suzuki M, Uchijima M, Hashimoto D, Rafiei A, Suda T, Chida K, Koide Y.
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Journal Title
Infection and Immunity 76 (4)
Pages: 1565-1571
Related Report
Peer Reviewed
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