Establishment of a novel vaccine against Mycobacterium tuberculosis employing carbohydrate-coated liposomes

• Project/Area Number: 20590439
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: TSUJIMURA Kunio Hamamatsu University School of Medicine, 医学部, 准教授 (10227407)
• Co-Investigator(Kenkyū-buntansha): KOIDE Yukio 浜松医科大学, 医学部, 理事 (30126809) ; NAGATA Toshi 浜松医科大学, 医学部, 教授 (90275024) ; UCHIJIMA Masato 浜松医科大学, 医学部, 助教 (20252174) ; SETO Shintaro 浜松医科大学, 医学部, 助教 (50383203)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: ワクチン / 結核菌 / 糖鎖 / リポソーム / 結核 / オリゴマンノース / Th1

Research Abstract

A novel vaccine against Mycobacterium tuberculosis was established employing a tuberculosis-protective antigen MPT51 and oligomannose-coated liposomes. Antigen-specific T-cell responses and antibody production were effectively induced in mice vaccinated with this composition. In addition, induced cytokine profiles and antibody classes suggested that this newly formulated vaccine could elicit Th1-type immune responses known to be important for the protection against tuberculosis.

Research Products

• [Journal Article] Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. (2011)
  • Author(s): 瀬戸真太郎, 辻村邦夫, 小出幸夫
  • Journal Title: Traffic 12 Pages: 407-420
  • Peer Reviewed
• [Journal Article] A novel vaccine strategy to induce mycobacterial antigen-specific Thlresponses by utilizing the C-terminal domain of Heat Shock Protein 70. (2011)
  • Author(s): Uto T, Tsujimura K, Uchijima M, Seto S, Nagata T, Suda T, Chida K, Nakamura H, Koide Y
  • Journal Title: FEMS Immunol Med Microbiol 61 Pages: 189-196
  • Peer Reviewed
• [Journal Article] 結核菌ファゴソームの成熟阻害機構 (2011)
  • Author(s): 瀬戸真太郎, 他
  • Journal Title: 化学療法の領域 Volume: (印刷中)
• [Journal Article] 結核菌の細胞内寄生メカニズム (2011)
  • Author(s): 瀬戸真太郎, 他
  • Journal Title: 日本臨床 Volume: (印刷中)
• [Journal Article] Rab GTPases regulating phagosome maturation are differentially recruited to mycobacterial phagosomes. (2011)
  • Author(s): Seto S, et al.
  • Journal Title: Traffic Volume: 12 Pages: 407-420
  • Peer Reviewed
• [Journal Article] A novel vaccine strategy to induce mycobacterial antigen-specific Th1 responses by utilizing the C-terminal domain of Heat Shock Protein 70. (2011)
  • Author(s): Uto T, et al.
  • Journal Title: FEMS Immunol.Med.Microbiol. Volume: 61 Pages: 189-196
  • Peer Reviewed
• [Journal Article] Differences in CD4^+CD25^+ regulatory T cell-depletion between Babesia microti and Babesia rodhaini infections in mice. (2010)
  • Author(s): Yokoyama N, Ikehara Y, Tiwananthagorn W, Ota N, Igarashi I, Kojima N, Taguchi O, Tsujimura K
  • Journal Title: J Protozool Res 20 Pages: 20-30
  • Peer Reviewed
• [Journal Article] Immunogenicity of dormancy-related antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Yamamura Y, Seto S, Uchijima M, Hozumi H, Nagata T, Tsujimura K, Koide Y
  • Journal Title: Procedia Vaccinology 3 Pages: 19-26
  • Peer Reviewed
• [Journal Article] Induction of specific CD8 T cells against intracellular bacteria by CD8 T-cell-oriented immunization approaches. (2010)
  • Author(s): 永田年, 小出幸夫
  • Journal Title: J.Biomed.Biotechnol. Pages: 764542-764542
  • Peer Reviewed
• [Journal Article] 結核菌に対するT細胞誘導ワクチンの試み (2010)
  • Author(s): 永田年、小出幸夫
  • Journal Title: 日本細菌学雑誌 65 Pages: 309-324
  • NAID: 10028050121
• [Journal Article] 結核菌抗原認識とT細胞免疫 (2010)
  • Author(s): 辻村邦夫、小出幸夫
  • Journal Title: 結核 85 Pages: 509-514
  • NAID: 10030262954
• [Journal Article] Identification of murine T-cell epitopes on low-molecular-mass secreted proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis. (2010)
  • Author(s): Eweda G, Suzuki D, Nagata T, Tsuiimura K, Koide Y
  • Journal Title: Vaccine 28 Pages: 4616-4625
  • Peer Reviewed
• [Journal Article] Differential recruitment of CD63 and Rab7-interacting-lysosomal-protein to phagosomes containing Mycobacterium tuberculosis in macrophages. (2010)
  • Author(s): Seto S, Matsumoto S, Tsujimura K, Koide Y
  • Journal Title: Microbiol Immunol 54 Pages: 170-174
  • NAID: 10029544344
  • Peer Reviewed
• [Journal Article] The herbal medicine compound falcarindiol from Notopterygii Rhizoma suppresses dendritic cell maturation. (2010)
  • Author(s): Mitsui S, Torii K, Fukui H, Tsujimura K, Maeda A, Nose M, Nagatsu A, Mizukami H, Morita A
  • Journal Title: J Pharmacol Exp Ther 333 Pages: 954-960
  • Peer Reviewed
• [Journal Article] Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secretory protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination. (2010)
  • Author(s): Wang L-X, Nagata T, Tsujimura K, Uchiiima M, Seto S, Koide Y
  • Journal Title: Vaccine 28 Pages: 2026-2031
  • Peer Reviewed
• [Journal Article] Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDPI) using DNA vaccination. (2010)
  • Author(s): Suzuki D, Nagata T, Eweda G, Matsumoto S, Matsumoto M, Tsujimura K, Koide Y
  • Journal Title: Vaccine 28 Pages: 2020-2025
  • Peer Reviewed
• [Journal Article] Etanercept treatment reduces the serum levels of interleukin- 15 and interferon-gamma inducible protein-10 in patients with rheumatoid arthritis. (2010)
  • Author(s): Ichikawa T, Kageyama Y, Kobayashi H, Kato N, Tsujimura K, Koide Y
  • Journal Title: Rheumatol Int 30 Pages: 725-730
  • Peer Reviewed
• [Journal Article] Differences in CD4^+CD25^+ regulatory T cell-depletion between Babesia microti and Babesia rodhaini infections in mice. (2010)
  • Author(s): Yokoyama N, et al.
  • Journal Title: J.Protozool.Res. Volume: 20 Pages: 20-30
  • Peer Reviewed
• [Journal Article] Immunogenicity of dormancy-related antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Yamamura Y, et al.
  • Journal Title: Procedia Vaccinology Volume: 3 Pages: 19-26
  • Peer Reviewed
• [Journal Article] Induction of specific CD8 T cells against intracellular bacteria by CD8 T-cell-oriented immunization approaches. (2010)
  • Author(s): Nagata T, et al.
  • Journal Title: J.Biomed.Biotechnol. Pages: 764542-764542
  • Peer Reviewed
• [Journal Article] 結核菌抗原認識とT細胞免疫 (2010)
  • Author(s): 辻村邦夫, 他
  • Journal Title: 結核 Volume: 85 Pages: 509-514
  • NAID: 10030262954
• [Journal Article] Identification of murine T-cell epitopes on low-molecular-mass secreted proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis. (2010)
  • Author(s): Eweda G, el al.
  • Journal Title: Vaccine Volume: 28 Pages: 4616-4625
  • Peer Reviewed
• [Journal Article] Differential recruitment of CD63 and Rab7-interacting-lysosomal-protein to phagosomes containing Mycobacterium tuberculosis in macrophages. (2010)
  • Author(s): Seto S, et al.
  • Journal Title: Microbiol.Immunol. Volume: 54 Pages: 170-174
  • NAID: 10029544344
  • Peer Reviewed
• [Journal Article] The herbal medicine compound falcarindiol from Notopterygii Rhizoma suppresses dendritic cell maturation. (2010)
  • Author(s): Mitsui S, et al.
  • Journal Title: J.Pharmacol.Exp.Ther. Volume: 333 Pages: 954-960
  • Peer Reviewed
• [Journal Article] Etanercept treatment reduces the serum levels of interleukin-15 and interferon-gamma inducible protein-10 in patients with rheumatoid arthritis (2010)
  • Author(s): Ichikawa T, et al.
  • Journal Title: Rheumatol Int 30 Pages: 725-730
  • Peer Reviewed
• [Journal Article] Characterization of murine T-cell epitopes on mycobacterial DNA-binding protein 1 (MDP1) using DNA vaccination (2010)
  • Author(s): Suzuki D, et al.
  • Journal Title: Vaccine 28 Pages: 2020-2025
  • Peer Reviewed
• [Journal Article] Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening and DNA vaccination (2010)
  • Author(s): Wang L-X, et al.
  • Journal Title: Vaccine 28 Pages: 2026-2031
  • Peer Reviewed
• [Journal Article] Interaction of lysosomal markers with phagosome containing Mycobacterium tuberculosis in macrophage (2010)
  • Author(s): Seto, et al.
  • Journal Title: Microbiol.Immunol. 54 Pages: 170-174
  • Peer Reviewed
• [Journal Article] The herbal medicine compound falcarindiol from Notopterygii Rhizoma suppresses dendritic cell maturation (2010)
  • Author(s): Mitsui, S, et al.
  • Journal Title: J Pharmacol Exp Ther (in press)
  • Peer Reviewed
• [Journal Article] A novel positive feedback loop between Cyclin-dependent kinase 1 (CDK1) and CHKI in the nucleus during the G2/M transition. (2009)
  • Author(s): Enomoto M, Goto H, Tomono Y, Kasahara K, Tsujimura K, Kiyono T, Inagaki M
  • Journal Title: J Biol Chem 284 Pages: 34223-34230
  • Peer Reviewed
• [Journal Article] Induction of anti-tumor immune responses with oligomannose-coated liposomes targeting to peritoneal macrophages. (2009)
  • Author(s): Tsujimura K, Ikehara Y, Nagata T, Koide Y, Kojima N
  • Journal Title: Procedia Vaccinology 1 Pages: 127-134
  • Peer Reviewed
• [Journal Article] Dissection of Rab7 localization on Mycobacterium tuberculosis phagosome. (2009)
  • Author(s): Seto S, Matsumoto S, Ohta I, Tsujimura K, Koide Y
  • Journal Title: Biochem Biophys Res Common 387 Pages: 272-277
  • Peer Reviewed
• [Journal Article] Mucosal vaccine using CTL epitope-pulsed dendritic cell confers protection for intracellular pathogen. (2009)
  • Author(s): Ozawa Y, Suda T, Hashimoto D, Hashimoto D, Nakamura Y, Enomoto N, Inui N, Koide Y, Nakamura H, Chida K.
  • Journal Title: Am J Respir Cell Mol Bird 41 Pages: 440-448
  • Peer Reviewed
• [Journal Article] HapMap scanning of novel human minor histocompatibility antigens. (2009)
  • Author(s): Kamei M, Nannya Y, Torikai H, Kawase T, Taura K, Inamoto Y, Takahashi T, Yazaki M, Morishima S, Tsuiimura K, Miyamura K, Ito T, Togari H, Riddell SR, Kodera Y, Morishima Y, Takahashi T, Kuzushima K, Ogawa S, Akatsuka Y
  • Journal Title: Blood 113 Pages: 5041-5048
  • Peer Reviewed
• [Journal Article] The cellular niche of Lesteria monocytogenes infection changes rapidly in the spleen. (2009)
  • Author(s): Aoshi T, Carrero JA, Konjufca V, Koide Y, Unanue ER, Miller MJ
  • Journal Title: Ear J Immunol 39 Pages: 417-425
  • Peer Reviewed
• [Journal Article] HapMap scanning of novel human minor histocompatibility antigens (2009)
  • Author(s): Kamei K, et al.
  • Journal Title: Blood 113 Pages: 5041-5048
  • Peer Reviewed
• [Journal Article] Mucosal vaccine using CTL epitope-pulsed dendritic cell confers protection for intracellular pathogen (2009)
  • Author(s): Ozawa Y, et al.
  • Journal Title: Am.J.Respir.Cell.Mol.Biol. 41 Pages: 440-448
  • Peer Reviewed
• [Journal Article] Dissection of Rab7 localization on Mycobacterium tuberculosis phagosome (2009)
  • Author(s): Seto S, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. 387 Pages: 272-277
  • Peer Reviewed
• [Journal Article] Induction of anti-tumor immune responses with oligomannose-coated liposomes targeting to peritoneal macrophages (2009)
  • Author(s): Tsujimura K, et al.
  • Journal Title: Procedia Vaccinology 1 Pages: 127-134
  • Peer Reviewed
• [Journal Article] A novel positive feedback loop between Cyclin-dependent kinase 1 (CDK1) and CHK1 in the nucleus during the G2/M transition (2009)
  • Author(s): Enomoto M, et al.
  • Journal Title: J.Biol.Chem. 284 Pages: 34223-34230
  • Peer Reviewed
• [Journal Article] The cellular niche of Listeria monocytogenes infection changes rapidlyin the spleen. (2009)
  • Author(s): Taiki Aoshi, et al.
  • Journal Title: European Journal of Immunology 39 Pages: 417-425
  • Peer Reviewed
• [Journal Article] Mucosal vaccine using cytotoxic T cell epitope-pulsed dendritic cell confers protection for intracellular pathogen. (2009)
  • Author(s): Yuichi Ozawa, et al.
  • Journal Title: American Journal of Respiratory Cell and molecular Biology (in press)
  • Peer Reviewed
• [Journal Article] HapMap scanning of novel human minor histocompatibility antigens. (2009)
  • Author(s): Michi Kamei, et al.
  • Journal Title: Blood (In press)
  • Peer Reviewed
• [Journal Article] Bacterial entry to the splenic white pulp initiates antigen presentation to CD8^+ T cells. (2008)
  • Author(s): Aoshi T, Zinselmeyer BH, Konjufca V, Lynch JN, Zhang X, Koide Y, Miller MJ
  • Journal Title: Immunity 29 Pages: 476-486
  • Peer Reviewed
• [Journal Article] Chemokine receptor-mediated delivery of mycobacterial MPT51 protein efficiently induces antigen-specific T-cell responses. (2008)
  • Author(s): Uchiiima M, Nagata T, Koide Y
  • Journal Title: Vaccine 26 Pages: 5165-5169
  • Peer Reviewed
• [Journal Article] In vivo hierarchy of individual T-cell epitope-specific helper T-cell subset against an intracellular bacterium. (2008)
  • Author(s): Nagata T, Aoshi T, Uchiiima M, Koide Y
  • Journal Title: Vaccine 26 Pages: 5123-5127
  • Peer Reviewed
• [Journal Article] Intratracheal administration of third-generation lentivirus vector encoding MPT51 from Mycobacterium tuberculosis induces specific CD8^+ T-cell responses in the lung. (2008)
  • Author(s): Hashimoto D, Nagata T, Uchijima M, Seto S, Suda T, Chida K, Miyoshi H, Nakamura H, Koide Y
  • Journal Title: Vaccine 26 Pages: 5095-5100
  • Peer Reviewed
• [Journal Article] Identification of an HLA-A*0201-restricted T-cell epitope on the MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis, by MPT51 overlapping peptide screening (2008)
  • Author(s): Aoshi T, Nagata T, Suzuki M, Uchiiima M, Hashimoto D, Rafiei A, Suda T, Chida K, Koide Y
  • Journal Title: Infect human 76 Pages: 1565-1571
  • Peer Reviewed
• [Journal Article] Effect of CD4^+CD25^+ regulatory T cell-depletion on acute lethal infection of mice with Trypanosoma congolense. (2008)
  • Author(s): Namangala B, Yokoyama N, Ikehara Y, Taguchi O, Tsujimura K, Sugimoto C, Inoue N
  • Journal Title: J Vet Med Sci 70 Pages: 751-759
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus nuclear antigen 1-specific CD4^+ T cells directly kill Epstein-Barr virus-carrying natural killer and T cells. (2008)
  • Author(s): Demachi-Okamura A, Ito Y, Akatsuka Y, Tsujimura K, Morishima Y, Takahashi T, Kuzushima K
  • Journal Title: Cancer Sci 99 Pages: 1633-1642
  • NAID: 10024010005
  • Peer Reviewed
• [Journal Article] Identification of a human leukocyte antigen-A24-restricted T-cell epitope derived from interleukin-13 receptor α2 chain, a glioma-associated antigen. (2008)
  • Author(s): Shimato S, Natsume A, Wakabayashi T, Tsujimura K, Nakahara N, Ishii J, Ito M, Akatsuka Y, Kuzushima K, Yoshida J
  • Journal Title: J Neurosurg 109 Pages: 117-122
  • Peer Reviewed
• [Journal Article] Induction of regulatory dendritic cells by topical application of NF-KB decoy oligodeoxynucleotides. (2008)
  • Author(s): Isomura I Shintani Y, Yasuda Y, Tsujimura K, Morita A
  • Journal Title: Immunol Lett 119 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Oligomannose-coated liposomes as a therapeutic antigen-delivery and an adjuvant vehicle for induction of in vivo tumor immunity. (2008)
  • Author(s): Kojima N, Biao L, Nakayama T, Ishii M, Ikehara Y, Tsujimura K
  • Journal Title: J Control Release 129 Pages: 26-32
  • Peer Reviewed
• [Journal Article] Interleukin-2 potentiation of cetuximab antitumor activity for epidermal growth factor receptor-overexpressing gastric cancer xenografts through antibody-dependent cellular cytotoxicity. (2008)
  • Author(s): Ham M, Nakanishi H, Tsujimura K, Matsui M, Yatabe Y, Manabe T, Tatematsu M
  • Journal Title: Cancer Sci 99 Pages: 1471-1478
  • NAID: 10024009000
  • Peer Reviewed
• [Journal Article] The DNA demethylating agent 5-aza-2'- deoxycytidine activates NY-ESO-1 antigenicity in orthotopic human glioma. (2008)
  • Author(s): Natsume A, Wakabayashi T, Tsujimura K, Shimato S, Ito M, Kuzushima K, Kondo Y, Sekido Y, Kawatsura H, Narita Y, Yoshida J
  • Journal Title: Int J Cancer 122 Pages: 2542-2553
  • Peer Reviewed
• [Journal Article] Identification of human minor histocompatibility antigens based on genetic association with highly parallel genotyping of pooled DNA. (2008)
  • Author(s): Kawase T, Nanya Y, Torikai H, Yamamoto G, Onizuka M, Morishima S, Tsujimura K, Miyamura K, Kodera Y, Morishima Y, Takahashi T, Kuzushima K, Ogawa S, Akatsuka Y
  • Journal Title: Blood 111 Pages: 3286-3294
  • Peer Reviewed
• [Journal Article] Effective induction of anti-tumor immune responses with oligomannose-coated liposome targeting to intraperitoneal phagocytic cells (2008)
  • Author(s): Ikehara Y, Shiuchi N, Kabata-Ikehara S, Nakanishi H, Yokoyama N, Takagi H, Nagata T, Koide Y, Kuzushima K, Takahashi T, Tsujimura K, Kojima N
  • Journal Title: Cancer Lett 260 Pages: 137-145
  • Peer Reviewed
• [Journal Article] Effective induction of anti-tumor immune responses with oligomannose-coated liposome targeting to intraperitoneal phagocytic cells. (2008)
  • Author(s): Yuzuru Ikehara, et al.
  • Journal Title: Cancer Letters 260 Pages: 137-145
  • Peer Reviewed
• [Journal Article] Identification of human minor histocompatibility antigens based on genetic association with highly parallel genotyping of pooled DNA. (2008)
  • Author(s): Takakazu Kawase, et al.
  • Journal Title: Blood 111 Pages: 3286-3294
  • Peer Reviewed
• [Journal Article] The DNA demethylating agent 5-aza-2' deoxycytidine activates NY-ESO-1 antigenicity in orthotopic human glioma. (2008)
  • Author(s): Atsushi Natsume, et al.
  • Journal Title: International Journal of Cancer 122 Pages: 2542-2553
  • Peer Reviewed
• [Journal Article] Interleukin-2 potentiation of cetuximab antitumor activity for epidermal growth factor receptor-overexpressing gastric cancer xenografts through antibody-dependent cellular cytotoxicity. (2008)
  • Author(s): Masayasu Hara, et al.
  • Journal Title: Cancer Science 99 Pages: 1471-1478
  • Peer Reviewed
• [Journal Article] Oligomannose-coated liposomes as a therapeutic antigen-delivery and an adjuvant vehicle for induction of in vivo tumor immunity. (2008)
  • Author(s): Naoya Kojima, et al.
  • Journal Title: Journal of Control Release 129 Pages: 26-32
  • Peer Reviewed
• [Journal Article] Induction of regulatory dendritic cells by topical application of NF-きごκB decoy oligodeoxynucleotides. (2008)
  • Author(s): Iwao Isomura, et al.
  • Journal Title: Immunology Letters 119 Pages: 49-56
  • Peer Reviewed
• [Journal Article] Identification of a human leukocyte antigen-A24-iestricted T-cell epitope derived from interleukin-13 receptor a2 chain, a glioma-associated antigen. (2008)
  • Author(s): Shinji Shimato, et al.
  • Journal Title: Journal of Neurosurgery 109 Pages: 117-122
  • Peer Reviewed
• [Journal Article] Epstein-Barr virus nuclear antigen 1-specific CD4^+ T cells directly kill Epstein-Barr virus-carrying natural killer and T cells. (2008)
  • Author(s): Ayako Demachi-Okamura, et al.
  • Journal Title: Cancer Science 99 Pages: 1633-1642
  • Peer Reviewed
• [Journal Article] Effect of CD4^+CD25^+ regulatory T cell-depletion on acute lethal infection of mice with Trypanosoma congolense. (2008)
  • Author(s): Boniface Namangala, et al.
  • Journal Title: Journal of Veterinary Medical Science 70 Pages: 751-759
  • Peer Reviewed
• [Journal Article] Identification of an HLA-A^*0201-restricted T-cell epitope on the MPT51 protein, a major secreted protein derived from Mycobacterium tuber culosis by MPT51 overlapping peptide screening. (2008)
  • Author(s): Taiki Aoshi, et al.
  • Journal Title: Infection and Immunity 76 Pages: 1565-1571
  • Peer Reviewed
• [Journal Article] Intratracheal administration of third-generation lentivirus vector encoding MPT51 from Mycobacterium tuberculosis induces specific CD8+T-cell responses in the lung. (2008)
  • Author(s): Dai Hashimoto, et al.
  • Journal Title: Vaccine 26 Pages: 5095-5100
  • Peer Reviewed
• [Journal Article] In vivo hierarchy of individual T-cell epitope-specific helper T-cell subset against an intracellular bacterium. (2008)
  • Author(s): Toshi Nagata, et al.
  • Journal Title: Vaccine 26 Pages: 5123-5127
  • Peer Reviewed
• [Journal Article] Chemokine receptor-mediated delivery of mycobacterial MPT51 protein efficiently induced antigen-specific T-cell responses. (2008)
  • Author(s): Masato Uchijima, et al.
  • Journal Title: Vaccine 26 Pages: 5165-5169
  • Peer Reviewed
• [Journal Article] Bacterial entry to the splenic white pulp initiates antigen presentation to CD8^+ T cells. (2008)
  • Author(s): Taiki Aoshi, et al.
  • Journal Title: Immunity 29 Pages: 476-486
  • Peer Reviewed
• [Journal Article] 結核菌ファゴソームの成熟阻害機構
  • Author(s): 瀬戸真太郎、辻村邦夫、小出幸夫
  • Journal Title: 化学療法の領域 印刷中
• [Journal Article] 結核菌の細胞内寄生メカニズム
  • Author(s): 瀬戸真太郎、辻村邦夫、小出幸夫
  • Journal Title: 日本臨床 印刷中
• [Presentation] 結核菌ファゴソームのプロテオミクス解析 (2010)
  • Author(s): 瀬戸真太郎, 他
  • Organizer: 平成22年度中部乳酸菌研究会
  • Place of Presentation: 松本
  • Year and Date: 2010-11-26
• [Presentation] プロテオミクスによる結核菌ファゴソームにおける小胞輸送機構の解析 (2010)
  • Author(s): 瀬戸真太郎, 他
  • Organizer: 第93回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2010-10-21
• [Presentation] Immunogenicity of latency-associated antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Tsujimura K, et al.
  • Organizer: The 10th Kyungpook-Hamamatsu Joint Medical Symposium
  • Place of Presentation: 浜松
  • Year and Date: 2010-09-17
• [Presentation] Proteomic analysis revealed the interaction of endoplasmic reticulum with the phagosomes containing Mycobacterium tuberculosis in macrophages. (2010)
  • Author(s): Seto S, et al.
  • Organizer: The 10th Kyungpook-Hamamatsu Joint Medical Symposium
  • Place of Presentation: 浜松
  • Year and Date: 2010-09-17
• [Presentation] Immune responses against dormancy-associated antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Yamamura Y, et al.
  • Organizer: 45th US-Japan Conference on Tuberculosis and Leprosy
  • Place of Presentation: Cambridge/USA
  • Year and Date: 2010-07-15
• [Presentation] Proteomic analysis reveals the interaction of endoplasmic reticulum with the phagosome containing Mycobacterium tuberculosis. (2010)
  • Author(s): Koide Y, et al.
  • Organizer: 45th US-Japan Conference on Tuberculosis and Leprosy
  • Place of Presentation: Cambridge/USA
  • Year and Date: 2010-07-15
• [Presentation] イメージ解析とプロテオミクスによる結核菌ファゴソームの分子解剖 (2010)
  • Author(s): 瀬戸真太郎, 他
  • Organizer: 第85回結核病学会総会
  • Place of Presentation: 京都
  • Year and Date: 2010-05-21
• [Presentation] Identification of murine T-cell epitopes on low-molecular-mass secreted proteins of Mycobacterium tuberculosis (2010)
  • Author(s): Nagata T, et al.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Genetic fusion of Mycobacterium tuberculosis heat hock protein 70 to a mycobacterial antigen MPT51 augments the antigen-specific T cell responses (2010)
  • Author(s): Uto T, et al.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Immune responses against latency-associated antigens of Mycobacterium tuberculosis in DNA-vaccinated mice (2010)
  • Author(s): Yamamura Y, et al.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Genetic fusion of MIP-1α to MPT51 enhances antigen-specific CD8^+ T-cell responses (2010)
  • Author(s): Uchijima M, et al.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] Proteomic analysis revealed the interaction of Mycobacterium tuberculosis phagosome with ER (2010)
  • Author(s): 瀬戸真太郎, et al.
  • Organizer: 第83回日本細菌学会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] Immunogenicity of dormancy-related antigens of Mycobacterium tuberculosis in DNA-vaccinated mice (2010)
  • Author(s): Yamamura Y, et al.
  • Organizer: DNA vaccine 2010
  • Place of Presentation: New Orleans, U. S. A.
  • Year and Date: 2010-03-02
• [Presentation] Identification of murine T-cell epitopes on low-molecular-mass secreted proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis with DNA immunization (2010)
  • Author(s): Eweda G, et al.
  • Organizer: DNA vaccine 2010
  • Place of Presentation: New Orleans, U. S. A.
  • Year and Date: 2010-03-02
• [Presentation] Immunogenicity of latency-associated antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Tsuiimura K, Yamamura Y, Hozumi H, Seto S, Uchijima M, Koide Y
  • Organizer: The 10th Kyungpook-Hamamatsu Joint Medical Symposium.
  • Place of Presentation: 浜松
• [Presentation] Proteomic analysis revealed the interaction of endoplasmic reticulum with the phagosomes containing Mycobacterium tuberculosis in macrophages. (2010)
  • Author(s): Seto S, Tsuiimura K, Koide Y
  • Organizer: The 10th Kyungpook-Hamamatsu Joint Medical Symposium.
  • Place of Presentation: 浜松
• [Presentation] Immune responses against dormancy-associated antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Yamamura Y, Seto S, Uchijima M, Tsuiimura K, Koide Y
  • Organizer: 45th US-Japan Conference on Tuberculosis and Leprosy.
  • Place of Presentation: Cambridge/USA
• [Presentation] Proteomic analysis reveals the interaction of endoplasmic reticulum with the phagosome containing Mycobacterium tuberculosis. (2010)
  • Author(s): Koide Y, Seto S, Tsujimura K
  • Organizer: 45th US-Japan Conference on Tuberculosis and Leprosy.
  • Place of Presentation: Cambridge/USA
• [Presentation] Identification of murine T-cell epitopes on low-molecular-mass secreted proteins (CFP11, CFP17, and TB18.5) of Mycobacterium tuberculosis with DNA immunization. (2010)
  • Author(s): Eweda G, Suzuki D, Nagata T, Tsuiimura K, Koide Y
  • Organizer: DNA vaccine 2010.
  • Place of Presentation: New Orleans/USA
• [Presentation] Immunogenicity of dormancy-related antigens of Mycobacterium tuberculosis in DNA-vaccinated mice. (2010)
  • Author(s): Yamamura Y, Seto S, Uchijima M, Hodumi H, Nagata T, Tsuiimura K, Koide Y
  • Organizer: DNA vaccine 2010.
  • Place of Presentation: New Orleans/USA
• [Presentation] Immunogenicity of latency-associated antigens of Mycobacterium tuberculosis in DNA-vaccinated mice (2009)
  • Author(s): Yamamura Y, et al.
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-04
• [Presentation] 結核菌ファゴソームのプロテオミクスによる分子解剖 (2009)
  • Author(s): 瀬戸真太郎, 他
  • Organizer: 第92回日本細菌学会関東支部総会
  • Place of Presentation: 東京
  • Year and Date: 2009-11-06
• [Presentation] Chk1 phosphorylation by Cdk1 promotes mitotic entry (2009)
  • Author(s): Enomoto M, et al.
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Genetic fusion of heat-shock protein 70 to a mycobacterial antigen enhances the antigen-specific T cell responses (2009)
  • Author(s): Uto T, et al.
  • Organizer: Vaccine 3rd Global Congress
  • Place of Presentation: Singapore
  • Year and Date: 2009-10-05
• [Presentation] Alternative localization of Rab GTPases to phagosome containing Mycobacterium tuberculosis in inhibition of phagolysosome biogenesis (2009)
  • Author(s): 瀬戸真太郎, et al.
  • Organizer: 第92回日本細菌学会関東支部総会
  • Place of Presentation: 福岡
  • Year and Date: 2009-07-29
• [Presentation] Chk1 phosphorylation by Cdk1 promotes mitotic entry (2009)
  • Author(s): Enomoto M, et al.
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-06-03
• [Presentation] Cancer vaccine delivery using oligomannose coated liposomes (2009)
  • Author(s): Ikehara Y, et al.
  • Organizer: AACR 100th Annual Meeting
  • Place of Presentation: Denver, U. S. A.
  • Year and Date: 2009-04-19
• [Presentation] ケモカイン融合型DNAワクチンにより誘導される抗原特異的CD8+およびCD4十T細胞の解析. (2009)
  • Author(s): 内嶋雅人, 他
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 結核菌のHeat-shock protein 70(HSP70)を結核菌抗原MPT51に結合させた分子融合型DNAワクチンは抗原特異的T細胞を効率良く誘導する. (2009)
  • Author(s): 右藤智啓, 他
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] DNAワクチンを用いた結核菌低分子量分泌蛋白のマウスT細胞エピトープの同定. (2009)
  • Author(s): 永田年, 他
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] DNAワクチンを用いたMycobacterium DNA-binding protein 1 (MDP1)のマウスT細胞エピトープの同定. (2009)
  • Author(s): 鈴木大介, 他
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 結核菌によるフアゴソーム局在性Rab GTPaseの局在変化とフアゴリソソーム形成阻害機構の解析. (2009)
  • Author(s): 瀬戸真太郎, 他
  • Organizer: 第82回日本細菌学会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-12
• [Presentation] Genetic fusion of heat-shock protein 70 to a mycobacterial antigen enhances the antigen-specific T cell responses. (2009)
  • Author(s): Uto T, Uchijima M, Seto S, Nagata T, Suda T, Chida K, Nakamura H, Tsuiimura K, Koide Y
  • Organizer: Vaccine 3rd Global Congress.
  • Place of Presentation: Singapore/Republic of Singapore
• [Presentation] Alternative localization of Rab GTPases to phagosome containing Mycobacterium tuberculosis in inhibition of phagolysosome biogenesis. (2009)
  • Author(s): Seto S, Koide Y
  • Organizer: 44th US-Japan Conference on Tuberculosis and Leprosy.
  • Place of Presentation: 福岡
• [Presentation] Cancer vaccine delivery using oligomannose coated liposomes. (2009)
  • Author(s): Ikehara Y, Ikehara SK, Yokoyama N, Kuzushima K, Takahashi T, Kojima N, Tsuiimura K
  • Organizer: AACR 100th Annual Meeting.
• [Presentation] Mycobacterium tuberculosis modulates the network of Rab GTPases in attenuation of phagosome maturation. (2009)
  • Author(s): Seto S, Koide Y
  • Organizer: Keystone symposium.
  • Place of Presentation: Keystone/USA
• [Presentation] Effective induction of anti-tumor immune responses with oligomannose-coated liposomes targeting to intraperitoneal macrophages. (2008)
  • Author(s): Kunio Tsujimura, et al.
  • Organizer: Vaccine 2nd Global Congress
  • Place of Presentation: Boston
  • Year and Date: 2008-12-08
• [Presentation] Genetic fusion of heat-shock protein 70 to a mycobacterial antigen enhances the antigen-specific T cell responses. (2008)
  • Author(s): 右藤智啓, 他
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京 都
  • Year and Date: 2008-12-02
• [Presentation] Analysis of antigen-specific CD8^+ and CD4^+ T-cell responses induced by MIP-lα-MPT51 fusion DNA vaccination. (2008)
  • Author(s): Masato Uchijima, et al.
  • Organizer: 第38回日本免疫学会総会・学術集会
  • Place of Presentation: 京 都
  • Year and Date: 2008-12-02
• [Presentation] Chk1 phosphorylation by Cyclin-dependent kinase 1 promotes mitotic entry. (2008)
  • Author(s): Goto H, Enomoto M, Tomono Y, Kasahara K, Ikegami Y, Tsuiimura K, Kiyono T, Inagaki M
  • Organizer: 48th Annual Meeting of the American Society for Cell Biology.
  • Place of Presentation: San Francisco/USA
• [Presentation] Identification of HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening. (2008)
  • Author(s): Nagata T, Wang L-X, Koide Y
  • Organizer: DNA Vaccines 2008
  • Place of Presentation: Las Vegas/USA
• [Presentation] Characterization of routine T-cell epitopes on mycobacterial DNA-binding protein 1(MDPI). (2008)
  • Author(s): Suzuki D, Nagata T, Matsumoto S, Koide Y
  • Organizer: DNA Vaccines 2008
  • Place of Presentation: Las Vegas/USA
• [Presentation] Effective induction of anti-tumor immune responses with oligomannose-coated liposomes targeting to intraperitoneal macrophages. (2008)
  • Author(s): Tsuiimura K, Ikehara Y, Nagata T, Koide Y, Kojima N
  • Organizer: Vaccine 2nd Global Congress.
  • Place of Presentation: Boston/USA
• [Presentation] Mycobacterium tuberculosis modulates the network of Rab GTPases to inhibit phagolysosome biogenesis in macrophage. (2008)
  • Author(s): Seto S, Koide Y
  • Organizer: 第8回あわじ感染症・免疫フォーラム.
  • Place of Presentation: 淡路
• [Presentation] Live Mycobacterium tuberculosis inhibits phagolysosome biogenesis in macrophages by modulating localization of Rab GTPase proteins on its phagosomes. (2008)
  • Author(s): Seto S, Koide Y
  • Organizer: 43rd US-Japan conference on tuberculosis and leprosy.
  • Place of Presentation: Baltimore/USA
• [Presentation] The DNA demethylating agent 5-aza-2'- deoxycytidine induces the expression of cancer-testis antigens in human gliomas : epigenetic target for tumor immunotherapy. (2008)
  • Author(s): Natsume A, Wakabayashi T, Shimato S, Tsujimura K, Kuzushima K, Yoshida J
  • Organizer: The 17th International Conference on Brain Tumor Research and Therapy.
  • Place of Presentation: 函館
• [Presentation] Live Mycobacterium tuberculosis inhibits phagolysosome biogenesis in macrophages by modulating localization of Rab GTPase proteins on its phagosomes. (2008)
  • Author(s): Shintaro Seto, et al.
  • Organizer: 43^<rd> US-Japan conference on tuberculosis and leprosy
  • Place of Presentation: Baltimore
• [Presentation] Mycobacterium tuberculosis modulates the network of Rab GTPases to inhibit phagolysosome biogenesis in macrophage. (2008)
  • Author(s): Shintaro Seto, et al.
  • Organizer: 第8回あわじ感染症・免疫フォーラム
  • Place of Presentation: 淡路
• [Presentation] 結核菌感染マクロファージにおけるRab GTPaseの網羅的局在解析. (2008)
  • Author(s): 瀬戸真太郎, 他
  • Organizer: 第91回細菌学会関東支部総会
  • Place of Presentation: 千葉
• [Presentation] Characterization of murine T-cell epitopes on mycobacterial DNA-bindins protein 1 (MDP1). (2008)
  • Author(s): Daisuke Suzuki, et al.
  • Organizer: DNA Vaccines 2008
  • Place of Presentation: Las Vegas
• [Presentation] HLA-DR4-restricted T-cell epitope on MPT51 protein, a major secreted protein derived from Mycobacterium tuberculosis using MPT51 overlapping peptides screening. (2008)
  • Author(s): Toshi Nagata, et al.
  • Organizer: DNA Vaccines 2008
  • Place of Presentation: Las Vegas
• [Presentation] Chk1 phosphorylation by Cdkl1 promote mitotic entry. (2008)
  • Author(s): Masato Enomoto, et al.
  • Organizer: 第31回日本分子生物学会年会・第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
• [Presentation] Chkl phosphorylation by Cyclin-dependent kinase 1 promotes mitotic entry. (2008)
  • Author(s): Hidemasa Goto, et al.
  • Organizer: 48th Annual Meeting of the American Society for Cell Biology
  • Place of Presentation: San Francisco
• [Presentation] Mycobacterium tuberculosis modulates the network of Rab GTPases in attenuation of phagosome maturation. (2001)
  • Author(s): Shintaro Seto, et al.
  • Organizer: Keystone symposium
  • Place of Presentation: Keystone
• [Book] Handbook of Listeria monocytogenes.(Chapter 15.Anti-infective vaccine strategies.) (2008)
  • Author(s): Nagata T, Koide Y
  • Publisher: CRC Press.
• [Remarks] ホームページ等
• [Patent(Industrial Property Rights)] Liposome composition fbr immune induction (2009)
  • Inventor(s): Kojima N, Ikehara Y, Tsujimura K
  • Industrial Property Rights Holder: Tokai University Educational system, Aichi Prefecture
  • Filing Date: 2009-05-29
• [Patent(Industrial Property Rights)] Liposome composition for immune induction (2009)
  • Inventor(s): Kojima N, Ikehara Y, Tsujimura K
  • Industrial Property Rights Holder: Tokai University Educational system, Aichi Prefecture
  • Filing Date: 2009-10-30
• [Patent(Industrial Property Rights)] Liposome composition for immune induction (2008)
  • Inventor(s): Kojima N, Ikehara Y, Tsujimura K
  • Industrial Property Rights Holder: Tokai University Educational system, Aichi Prefecture
  • Filing Date: 2008-06-12