Role of macrophage in Helicobacter pylori infection

• Project/Area Number: 20590445
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Okayama University
• Principal Investigator: YOKOTA Kenji Okayama University, 大学院・保健学研究科, 准教授 (00243460)
• Co-Investigator(Kenkyū-buntansha): AYADA Kiyoshi 岡山大学, 大学院・医歯薬学総合研究科, 助教 (00379835) ; OGUMA Kenji 岡山大学, 大学院・医歯薬学総合研究科, 教授 (00002262)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: Helicobacter pylori / 胃ガン / DEC205 / Th1 / 2 / HSP60 / 冠動脈疾患 / Helicobacter / Macrophage / マクロファージ / HSP / CagA

Research Abstract

Expression of DEC205, which is antigen uptake receptor on macrophage, was increased in H.pylori infected patients with early gastric cancer. However mRNA of DEC205 was also increased in the patient without gastric cancer. Expression of Th1 cytokines was detected in lymphocyte stimulated with HSP60 from patients with gastric cancer. HSP60 stimulation and its response in T helper CD4 may be available for diagnosis or prediction of gastric cancer caused by H.pylori infection. On the other hand, HSP60 antibody may be associated with cardiovascular disease (CVD) induced by H.pylori infection.

Research Products

• [Journal Article] The long-term risk of gastric cancer after the successful eradication of Helicobacter pylori. (2011)
  • Author(s): Take S, Mizuno M, Ishiki K, Yoshida T, Ohara N, Yokota K, Oguma K, Okada H, Yamamoto K
  • Journal Title: J Gastroenterol. Volume: 46 Pages: 318-324
  • NAID: 10029027739
  • Peer Reviewed
• [Journal Article] Behcet's disease (Adamantiades-Behcet's disease). (2010)
  • Author(s): Kaneko F, Togashi A, Saito S, Sakuma H, Oyama N, Nakamura K, Yokota K, Oguma K.
  • Journal Title: Clin Dev Immunol. 2011 Review. Pages: 681956-681956
  • Peer Reviewed
• [Journal Article] Induction of antibacterial activity in larvae of the blowfly Lucilia sericata by an infected environment. (2010)
  • Author(s): Kawabata T, Mitsui H, Yokota K, Ishino K, Oguma K, Sano S.
  • Journal Title: Med Vet Entomol. 24(4) Pages: 375-381
  • Peer Reviewed
• [Journal Article] The long-term risk of gastric cancer after the successful eradication of Helicobacter pylori. (2010)
  • Author(s): Take S, Mizuno M, Ishiki K, Yoshida T, Ohara N, Yokota K, Oguma K, Okada H, Yamamoto K.
  • Journal Title: J Gastroenterol. 46(3) Pages: 318-324
  • NAID: 10029027739
  • Peer Reviewed
• [Journal Article] Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. (2010)
  • Author(s): Hirai K, Arimitsu H, Umeda K, Yokota K, Shen L, Ayada K, Kodama Y, Tsuji T, Hirai Y, Oguma K.
  • Journal Title: Acta Med Okayama. 64(3) Pages: 163-170
  • NAID: 120002311046
  • Peer Reviewed
• [Journal Article] Induction of antibacterial activity in larvae of the blowfly Lucilia sericata by an infected environment. (2010)
  • Author(s): Kawabata T, Mitsui H, Yokota K, Ishino K, Oguma K, Sano S.
  • Journal Title: Med Vet Entomol. Volume: 24 Pages: 375-381
  • Peer Reviewed
• [Journal Article] Passive oral immunization by egg yolk immunoglobulin (IgY) to Vibrio cholerae effectively prevents cholera. (2010)
  • Author(s): Hirai K, Arimitsu H, Umeda K, Yokota K, Shen L, Ayada K, Kodama Y, Tsuji T, Hirai Y, Oguma K.
  • Journal Title: Acta Med Okayama. Volume: 64 Pages: 163-170
  • NAID: 120002311046
  • Peer Reviewed
• [Journal Article] Detection of Helicobacter hepaticus in human bile samples of patients with biliary disease. (2009)
  • Author(s): Hamada T, Yokota K, Ayada K, Hirai K, Kamada T, Haruma K, Chayama K, Oguma K.
  • Journal Title: Helicobacter. 14(6) Pages: 545-551
  • Peer Reviewed
• [Journal Article] Regulation of cellular immunity prevents Helicobacter pylori-induced atherosclerosis. (2009)
  • Author(s): Ayada K, Yokota K, Hirai K, Fujimoto K, Kobayashi K, Ogawa H, Hatanaka K, Hirohata S, Yoshino T, Shoenfeld Y, Matsuura E, Oguma K.
  • Journal Title: Lupus. 18(13) Pages: 1154-1168
  • Peer Reviewed
• [Journal Article] Steroids mediate resistance to the bactericidal effect of phosphatidylcholines against Helicobacter pylori. (2009)
  • Author(s): Shimomura H, Hosoda K, Hayashi S, Yokota K, Oguma K, Hirai Y.
  • Journal Title: FEMS Microbiol Lett. 301(1) Pages: 84-94
  • Peer Reviewed
• [Journal Article] Immune reactions against elongation factor 2 kinase : specific pathogenesis of gastric ulcer from Helicobacter pylori infection. (2009)
  • Author(s): Ayada K, Yokota K, Kawahara Y, Yamamoto Y, Hirai K, Inaba T, Kita M, Okada H, Yamamoto K, Oguma K.
  • Journal Title: Clin Dev Immunol. 2009 Pages: 850623-850623
  • Peer Reviewed
• [Journal Article] Anabolic utilization of steroid hormones in Helicobacter pylori. (2009)
  • Author(s): Hosoda K, Shimomura H, Hayashi S, Yokota K, Oguma K, Hirai Y.
  • Journal Title: FEMS Microbiol Lett. 297(2) Pages: 173-179
  • Peer Reviewed
• [Journal Article] Helicobacter pylori heat shock protein 60 antibodies are associated with gastric cancer. (2009)
  • Author(s): Tanaka A, Kamada T, Yokota K, Shiotani A, Hata J, Oguma K, Haruma K.
  • Journal Title: Pathol Res Pract. 205(10) Pages: 690-694
  • Peer Reviewed
• [Journal Article] Chronic infections and atherosclerosis. (2009)
  • Author(s): Ayada K, Yokota K, Kobayashi K, Shoenfeld Y, Matsuura E, Oguma K.
  • Journal Title: Clin Rev Allergy Immunol. 37(1) Review. Pages: 44-48
  • Peer Reviewed
• [Journal Article] Helicobacter pylori eradication may induce de novo, but transient and mild, reflux esophagitis : Prospective endoscopic evaluation. (2009)
  • Author(s): Take S, Mizuno M, Ishiki K, Nagahara Y, Yoshida T, Yokota K, Oguma K, Okada H, Yamamoto K.
  • Journal Title: J Gastroenterol Hepatol. 24(1) Pages: 107-113
  • Peer Reviewed
• [Journal Article] Detection of Helicobacter hepaticus in human bile samples of patients with biliary disease. (2009)
  • Author(s): Hamada T, Yokota K, Ayada K, Hirai K, Kamada T, Haruma K, Chayama K, Oguma K.
  • Journal Title: Helicobacter 14 Pages: 545-551
  • Peer Reviewed
• [Journal Article] Regulation of cellular immunity prevents Helicobacter pylori-induced atherosclerosis. (2009)
  • Author(s): Ayada K, Yokota K, Hirai K, Fujimoto K, Kobayashi K, Ogawa H, Hatanaka K, Hirohata S, Yoshino T, Shoenfeld Y, Matsuura E,
  • Journal Title: Lupus 18 Pages: 1154-1154
  • Peer Reviewed
• [Journal Article] Steroids mediate resistance to the bactericidal effect of phosphatidylcholines against Helicobacter pylori. (2009)
  • Author(s): Shimomura H, Hosoda K, Hayashi S, Yokota K, Oguma K, Hirai Y
  • Journal Title: FEMS Microbiol Lett 301 Pages: 84-94
  • Peer Reviewed
• [Journal Article] Immune reactions against elongation factor 2 kinase : specific pathogenesis of gastric ulcer from Helicobacter pylori infection. (2009)
  • Author(s): Ayada K, Yokota K, Kawahara Y, Yamamoto Y, Hirai K, Inaba T, Kita M, Okada H, Yamamoto K, Oguma K.
  • Journal Title: Clin Dev Immunol 2009 Pages: 623-633
  • Peer Reviewed
• [Journal Article] Anabolic utilization of steroid hormones in Helicobacter pylori. (2009)
  • Author(s): Hosoda K, Shimomura H, Hayashi S, Yokota K, Oguma K, Hirai Y.
  • Journal Title: FEMS Microbiol Lett. 297 Pages: 179-179
  • Peer Reviewed
• [Journal Article] Helicobacter pylori heat shock protein 60 antibodies are associated with gastric cancer. (2009)
  • Author(s): Tanaka A, Kamada T, Yokota K, Shiotani A, Hata J, Oguma K, Haruma K.
  • Journal Title: Pathol Res Pract 205 Pages: 609-614
  • Peer Reviewed
• [Journal Article] Chronic infections and atherosclerosis. (2009)
  • Author(s): Ayada K, Yokota K, Kobayashi K, Shoenfeld Y, Matsuura E, Oguma K.
  • Journal Title: Clin Rev Allergy Immunol 37 Pages: 44-48
  • Peer Reviewed
• [Journal Article] Helicobacter pylori eradication may induce de novo, but transient and mild, reflux esophagitis : Prospective endoscopic evaluation. (2009)
  • Author(s): Take S, Mizuno M, Ishiki K, Nagahara Y, Yoshida T, Yokota K, Oguma K, Okada H, Yamamoto K.
  • Journal Title: J Gastroenterol Hepatol 24 Pages: 107-113
  • Peer Reviewed
• [Journal Article] Helicobacter pylori eradication may induce de novo, but transient and mild, reflux esophagitis : Prospective endoscopic evaluation. (2009)
  • Author(s): Take s, Mizuno M, Ishiki K
  • Journal Title: J Gastroenterol Hepatol 24 Pages: 107-113
  • Peer Reviewed
• [Journal Article] The role of streptococcal hypersensitivity in the pathogenesis of Behcet's Disease. (2008)
  • Author(s): Kaneko F, Oyama N, Yanagihori H, Isogai E, Yokota K, Oguma K.
  • Journal Title: Eur J Dermatol. 18(5) Pages: 489-98
  • Peer Reviewed
• [Journal Article] Chronic Infections and Atherosclerosis. (2008)
  • Author(s): Ayada K, Yokota K, Kobayashi K
  • Journal Title: Clin Rev Allergy Immunol 5 Pages: 1007-1012
  • Peer Reviewed
• [Presentation] Helicobacter infection and Cancer Disease, Immune reactions in MALT lymphoma and Gastric Cancer (2011)
  • Author(s): Yokota, K
  • Organizer: The 3rd International Symposium for Future Technology Creating Better Human Health and Society <Molecular Targets for the Development of Therapeutic Agents against Cancer and Infectious Diseases in vitro, in vivo and in silico>
  • Place of Presentation: Okayama
• [Presentation] H.pylori感染と冠動脈疾患の関係 (2010)
  • Author(s): 横田憲治、山本由弥子、鈴木智典、長町栄子、小熊惠二
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
• [Presentation] ベーチェット病患者由来熱ショックタンパクHSP60由来ペプチドの免疫活性の研究 (2010)
  • Author(s): 大野佑子、横田憲治、申蓮花、山本由弥子、小熊惠二
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
• [Presentation] Helicobacter pyloriの除菌後再感染菌の検討 (2010)
  • Author(s): 西田奈々、大野佑子、横田憲治、山本由弥子、鈴木智典、小熊惠二
  • Organizer: 第63回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
• [Presentation] H.pylori除菌後の再感染についての検討 (2010)
  • Author(s): 武進、水野元夫、石木邦治、喜多雅英、横田憲治、小熊惠二
  • Organizer: 第16回日本ヘリコバクター学会
  • Place of Presentation: 京都
• [Presentation] ステロイドの抗ピロリ菌活性 (2010)
  • Author(s): 細田浩一、下村裕史、林俊治、横田憲治、小熊惠二、平井義一
  • Organizer: 第16回日本ヘリコバクター学会
  • Place of Presentation: 京都
• [Presentation] H.pylori感染と冠動脈疾患の関係 (2010)
  • Author(s): 横田憲治、喜多雅英、綾田潔、林俊治、平井義一、小熊惠二
  • Organizer: 第16回日本ヘリコバクター学会
  • Place of Presentation: 京都
• [Presentation] ピロリ菌におけるステロイドホルモンの同化的利用 (2010)
  • Author(s): 細田浩一、下村裕史、林俊治、横田憲治、小熊惠二、平井義一
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] ステロイド吸収によって惹起されるピロリ菌のフォスファチジールコリン耐性の発現 (2010)
  • Author(s): 下村裕史、細田浩一、林俊治、横田憲治、小熊惠二、平井義一
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Helicobacter pyloriの病原遺伝子の多様性と胃粘膜萎縮の関係についての検討 (2010)
  • Author(s): 横田憲治、大野佑子、林俊治、平井義一、長町英子、小熊惠二
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
• [Presentation] Helicobacter infection and Cancer Disease, Immune reactions in MALT lymphoma and Gastric Cancer (2010)
  • Author(s): 横田憲治
  • Organizer: The 3rd International Symposium for Future Technology Creating Better Human Health and Society <Molecular Targets for the Development of Therapeutic Agents against Cancer and Infectious Diseases in vitro, in vivo and in silico>
  • Place of Presentation: Okayama
• [Presentation] H.pylori除菌後の再感染についての検討 (2010)
  • Author(s): 武進、水野元夫、石木邦治、喜多雅英、横田憲治、小熊惠二
  • Organizer: 第16回日ヘリコバクター学会
  • Place of Presentation: 京都
• [Presentation] H.pylori感染と冠動脈疾患の関係 (2010)
  • Author(s): 横田憲治、山本由弥子、鈴木智典、長町栄子、小熊惠二
  • Organizer: 第63回 日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
• [Presentation] ベーチェット病患者由来熱ショックタンパクHSP60由来ペプチドの免疫活性の研究 (2010)
  • Author(s): 大野佑子、横田憲治、申蓮花、山本由弥子、小熊惠二
  • Organizer: 第63回 日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
• [Presentation] Helicobacter pyloriの除菌後再感染菌の検討 (2010)
  • Author(s): 西田奈々、大野佑子、横田憲治、山本由弥子、鈴木智典、小熊惠二
  • Organizer: 第63回 日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
• [Presentation] Helicobacter pylori infection may cause the atherosclerosis via Thl-dominant reactions induced HSP60. Cell Stress Society International (2009)
  • Author(s): Keiji Oguma, Dwi Fatmawati, Kiyoshi Ayada, Kenji Yokota, Kazuyuki Hirai, Masahide Kita, Eiji Matsuura, Shouzou Kusachi.
  • Organizer: The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: Sapporo, Japan.
• [Presentation] Helicobacter pylori infection may cause the atherosclerosis via Thl-dominant reactions induced mainly by both bacterial- and host- HSP60s. (2009)
  • Author(s): Keiji Oguma, Kiyoshi Ayada, Kenji Yokota, Masahide Kita, Dwi Fatmawati, Eiji Matsuura, Shouzou Kusachi
  • Organizer: International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO).
  • Place of Presentation: Niigata
• [Presentation] Detection of Helicobacter hepaticus in human bile samples of patients with biliary disease. (2009)
  • Author(s): Kenji Yokota, Kiyoshi Ayada, Toshihide Hamada, Kazuyuki Hirai, Tomoari Kamada, Ken Haruma, Keiji Oguma
  • Organizer: International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO).
  • Place of Presentation: Niigata
• [Presentation] Anabolic utilization of steroid hormones in Helicobacter pylori. (2009)
  • Author(s): Kouichi Hosoda, Hirofumi Shimomura, Shunji Hayashi, Kenji Yokota, Keiji Oguma, Yoshikazu Hirai
  • Organizer: International Workshop on Campylobacter, Helicobacter, and Related Organisms (CHRO).
  • Place of Presentation: Niigata
• [Presentation] Helicobacter pylori感染動脈硬化モデルマウスにおけるT細胞の関与 (2009)
  • Author(s): 綾田潔、横田憲治、平井一行、喜多雅英、小熊惠二
  • Organizer: 第15回日本ヘリコバクター学会
  • Place of Presentation: 東京
• [Presentation] Helicobacter pylori菌の病原遺伝子の多様性と疾患についての検討 (2009)
  • Author(s): 喜多雅英、横田憲治、岡田裕之、武進、綾田潔、竹中龍太、河原祥朗、山本和秀、小熊惠二
  • Organizer: 第15回日本ヘリコバクター学会
  • Place of Presentation: 東京
• [Presentation] Helicobacter pyloriによる培養細胞からのサイトカイン誘導性の検討 (2009)
  • Author(s): 横田憲治、綾田潔、武進、水野元夫、小熊惠二
  • Organizer: 第15回日本ヘリコバクター学会
  • Place of Presentation: 東京
• [Presentation] H.pylori抗原刺激による胃ガン患者の末梢血マクロファージレセプターDEC205の発現解析 (2009)
  • Author(s): 横田憲治、綾田潔、喜多雅英、岡田裕之、山本和秀、小熊惠二
  • Organizer: 第15回日本ヘリコバクター学会
  • Place of Presentation: 東京
• [Presentation] Helicobacter pylori infection may cause the atherosclerosis via Thl-dominant reactions induced mainly by both bacterial-and host-HSP60s. (2009)
  • Author(s): Keiji Oguma, Kiyoshi Ayada, Kenji Yokota, Masahide Kita, Dwi Fatmawati, Eiji Matsuura, Shouzou Kusachi,
  • Organizer: International Workshop on Campylobacter, Helicobacter, and Related Organisms(CHRO).
  • Place of Presentation: Niigata
• [Presentation] Detection of Helicobacter hepaticus in human bile samples of patients with biliary disease. (2009)
  • Author(s): Kenji Yokota, Kiyoshi Ayada, Toshihide Hamada, Kazuyuki Hirai, Tomoari Kamada, Ken Haruma, Keiji Oguma.
  • Organizer: International Workshop on Campylobacter, Helicobacter, and Related Organisms(CHRO).
  • Place of Presentation: Niigata
• [Presentation] Anabolic utilization of steroid hormones in Helicobacter pylori. (2009)
  • Author(s): Kouichi Hosoda, Hirofumi Shimomura, Shunji Hayashi, Kenji Yokota, Keiji Oguma, Yoshikazu Hirai.
  • Organizer: International Workshop on Campylobacter, Helicobacter, and Related Organisms(CHRO).
  • Place of Presentation: Niigata
• [Presentation] Helicobacter pylori infection may cause the atherosclerosis via Thl-dominant reactions induced HSP60. (2009)
  • Author(s): Keiji Oguma, Dwi Fatmawati, Kiyoshi Ayada, Kenji Yokota, Kazuyuki Hirai, Masahide Kita, Eiji Matsuura, Shouzou Kusachi.
  • Organizer: Cell Stress Society International, The 4th International Congress on Stress Responses in Biology and Medicine
  • Place of Presentation: Sapporo, Japan.
• [Presentation] H. pylori抗原刺激による胃ガン患者の末梢血マクロファージレセプターDEC205の発現解析 (2009)
  • Author(s): 横田憲治、綾田潔、喜多雅英、岡田裕之、山本和秀、小熊恵二
  • Organizer: 第15回日本ヘリコバクター学会
  • Place of Presentation: 東京
• [Presentation] Helicobacter pyloriによる培養細胞からのサイトカイン誘導能の検討 (2009)
  • Author(s): 衛藤友美, 横田憲治, 綾田潔
  • Organizer: 第82回日本棚菌学会総会
  • Place of Presentation: 名古屋