Antigen presentation and cytokine resposes against mycobial glycolipids.

• Project/Area Number: 20590453
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bacteriology (including Mycology)
• Research Institution: Iwaki Meisei University
• Principal Investigator: KUMAZAWA Yoshio Iwaki Meisei University, 薬学部, 客員研究員 (30072375)
• Co-Investigator(Kenkyū-buntansha): TAKIMOTO Hiroaki 北里大学, 理学部, 講師 (00253534)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 感染免疫 / 結核菌糖脂質 / モルモット / サイトカイン / IL-12 / IFN-γ

Research Abstract

The present study has aimed at the antigen presentation and cytokine responses against mycobacterial glycolipids using the guinea pig. Guinea pigs were inoculated intradermally with M.bovis BCG. Mycobacterial lipids specific serum IgG antibody titer was measured by ELISA. BCG-infected guinea pigs had IgG responses against PPD and phosphatidylinositol mannoside (PIM). Proliferation of T cells specific for mycobacterial lipids was estimated by tritiated-thymidine uptake. In BCG-infected guinea pig, splenic T cells showed proliferative responses to PPD, PIM and trehalose 6,6'-dimicolate (TDM).

Research Products

• [Journal Article] Suppression of inflammatory responses after onset of collagen-induced arthritis in mice by oral administration of the Citrus flavanone naringin. (2011)
  • Author(s): Kawaguchi K, Maruyama H, Hasunuma R, Kumazawa Y.
  • Journal Title: Immunopharmacol Immunotoxicol. in press
• [Journal Article] Effects of Antioxidant Polyphenols on TNF-alpha-Related Diseases. (2011)
  • Author(s): Kawaguchi K, Matsumoto T, Kumazawa Y.
  • Journal Title: Curr Top Med Chem. in press
• [Journal Article] Natural Antioxidants and their Derivatives : Biology and Clinical Application. (2011)
  • Author(s): Magrone T, Kumazawa Y, Jirillo E.
  • Journal Title: Curr Top Med Chem. in press
• [Journal Article] Inhibitory effects of fermented grape marc from Vitis vinifera Negroamaro on antigen-induced degranulation. (2010)
  • Author(s): Kaneko M, Kanesaka M, Yoneyama M, Tominaga T, Jirillo E, Kumazawa Y.
  • Journal Title: Immunopharmacol Immunotoxicol. PubMed PMID : 20100066 32(3) Pages: 454-461
• [Journal Article] Suppression of type-I allergic responses by oral administration of grape marc fermented with Lactobacillus plantarum. (2010)
  • Author(s): Tominaga T, Kawaguchi K, Kanesaka M, Kawauchi H, Jirillo E, Kumazawa Y.
  • Journal Title: Immunopharmacol Immunotoxicol. 32(4) Pages: 593-599
• [Journal Article] Liposomal glycosphingolipids activate natural killer T cell-mediated immune responses through the endosomal pathway. (2009)
  • Author(s): Inoue J, Ideue R, Takahashi D, Kubota M, Kumazawa Y.
  • Journal Title: J Control Release. 133(1) Pages: 18-23
• [Journal Article] Potentiation of murine innate immunity by alpha-galacturonosyl-type glycosphingolipids isolated from Sphingomonas yanoikuyae and S. terrae. (2009)
  • Author(s): Kubota M, Takimoto H, Kaneko M, Inoue J, Kumazawa Y.
  • Journal Title: Immunopharmacol Immunotoxicol. 31(3) Pages: 363-369
• [Journal Article] Amelioration of skewed Th1/Th2 balance in tumor-bearing and asthma-induced mice by oral administration of Agaricus blazei extracts. (2008)
  • Author(s): Takimoto H, Kato H, Kaneko M, Kumazawa Y.
  • Journal Title: Immunopharmacol Immunotoxicol. 30(4) Pages: 747-760
• [Journal Article] Suppressive effects of the flavonoids quercetin and luteolin on the accumulation of lipid rafts after signal transduction via receptors. (2008)
  • Author(s): Kaneko M, Takimoto H, Sugiyama T, Seki Y, Kawaguchi K, Kumazawa Y.
  • Journal Title: Immunopharmacol Immunotoxicol. 30(4) Pages: 867-882
• [Presentation] 結核菌糖脂質trehalose 6, 6'-dimicolate (TDM)による肉芽腫形成におけるγδ型T細胞の役割 (2010)
  • Author(s): 齋藤昂良、滝本博明、矢野郁也、熊沢義雄、服部雅一
  • Organizer: 第83回日本細菌学会総会 [日本細菌学雑誌第65巻(1):183]
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-29
• [Presentation] 結核菌糖脂質trehalose 6,6'-dimicolate(TDM)による肉芽腫形成におけるγδ型T細胞の役割 (2010)
  • Author(s): 齋藤昂良、滝本博明、矢野郁也、態沢義雄、服部雅一
  • Organizer: 日本細菌学会
  • Place of Presentation: パシフィコ横浜会議センター(神奈川県)
• [Presentation] 結核菌糖脂質trehalose 6, 6'-dimicolateによる肉芽腫形成におけるIL-17産生γδ型T細胞の役割 (2009)
  • Author(s): 齋藤昂良、滝本博明、矢野郁也、熊沢義雄
  • Organizer: 第92回日本細菌学会関東支部総会 [第92回日本細菌学会関東支部総会講演抄録集p.42]
  • Place of Presentation: 東京
  • Year and Date: 2009-11-05
• [Presentation] 結核菌糖脂質trehalose 6, 6'-dimycolate (TDM)による炎症誘導作用 (2009)
  • Author(s): 齋藤昴良、滝本博明、矢野郁也、熊沢義雄
  • Organizer: 第82回日本細菌学会総会 [日本細菌学雑誌第64巻(1):191]
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 結核菌糖脂質trehalose 6, 6'-dimycolate (TDM)による炎症誘導作用 (2008)
  • Author(s): 齋藤昴良、滝本博明、矢野郁也、熊沢義雄
  • Organizer: 第38回日本免疫学会総会学術集会 [日本免疫学会学術集会記録第38巻:158]
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02