Investigation of Inhibitory effect of Extra-Intestinal Pathogenic Escherichia coli against human neutrophils

Research Project

Project/Area Number	20590463
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Bacteriology (including Mycology)
Research Institution	National Institute of Infectious Diseases
Principal Investigator	OHNISHI Makoto National Institute of Infectious Diseases, 細菌第一部, 部長 (10233214)
Co-Investigator(Kenkyū-buntansha)	ATO Manabu 国立感染症研究所, 免疫部, 室長 (20392318)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000) Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000) Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	大腸菌 / 好中球 / 病原性 / 腸管外病原性 / 血清耐性 / 補体 / 系統
Research Abstract	Molecular mechanism of Extra-Intestinal pathogenic Escherichia coli was investigated. The strain CK17, which is UPEC O2, showed extra high resistant to serum treatment and also resistant to human neutrophils phagocytosis. K1 capsule was responsible to PMN resistant, but not serum resistance. C3 molecule and IgG could not bind K1-produceing CK17. The mechanism of K1-independent serum resistant remains unsolved.