Regulation of SARS-CoV replication by cyclophilin

• Project/Area Number: 20590467
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Juntendo University
• Principal Investigator: YAMAMOTO Norio Juntendo University, 医学部, 助教 (40323703)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: SARSコロナウイルス / サイクロスポリンA / コロナウイルス / サイクロフィリン

Research Abstract

To investigate the relationship between cyclophilin and SARS-CoV replication, we introduced shRNA expression vectors to cells and compare the virus titer produced from these cells. Efficiency of SARS-CoV replication was much lower in the cells expressing shRNA against cyclophilin A and B than in the cells expressing control shRNA. The results of time-of-addition assay and entry assay showed that cyclosporin A inhibited SARS-CoV replication at the post-entry step in viral life cycle. These results suggested that cyclophilins could play a role as positive regulators of SARS-CoV replication at the post-entry step.

Research Products

• [Journal Article] HIV-1 RT-dependent DNAzyme expression inhibits HIV-1 replication without the emergence of escape viruses. (2011)
  • Author(s): Sugiyama R, Hayafune M, Habu Y, Yamamoto N, Takaku H
  • Journal Title: Nucleic Acids Research Volume: 39 Pages: 589-598
  • Peer Reviewed
• [Journal Article] TACE antagonists blocking ACE2 shedding caused by the spike protein of SARS-CoV are candidate antiviral compounds. (2010)
  • Author(s): Haga S, Nagata N, Okamura T, Yamamoto N, Sata T, Yamamoto N, Sasazuki T, Ishizaka Y
  • Journal Title: Antiviral Resarch 85(3) Pages: 551-5
• [Journal Article] TACE antagonists blocking ACE2 shedding caused by the spike protein of SARS-CoV are candidate antiviral compounds. (2010)
  • Author(s): Haga S, Nagata N, Okamura T, Yamamoto N, Sata T, Yamamoto N, Sasazuki T, Ishizaka Y.
  • Journal Title: Antiviral Research Volume: 85 Pages: 551-555
  • Peer Reviewed
• [Journal Article] TACE antagonists blocking ACE2 shedding caused by the spike protein of SARS-CoV are candidate antiviral compounds (2010)
  • Author(s): Haga S, Nagata N, Okamura T, Yamamoto N, Sata T, Yamamoto N, Sasazuki T, Ishizaka Y.
  • Journal Title: Antiviral Research 85 Pages: 551-555
  • Peer Reviewed
• [Journal Article] Modulation of TNF-alpha-converting enzyme by the spike protein of SARS-CoV and ACE2 induces TNF-alpha production and facilitates viral entry. (2008)
  • Author(s): Haga S, Yamamoto N, Nakai-Murakami C, Osawa Y, Tokunaga K, Sata T, Yamamoto N, Sasazuki T, Ishizaka Y
  • Journal Title: Proc Natl Acad Sci U S A 105(22) Pages: 7809-14
• [Journal Article] Modulation of TNF-alpha-converting enzyme by the spikeprotein of SARS-CoV and ACE2 induces TNF-alpha production and facilitates viral entry. (2008)
  • Author(s): Haga S, Yamamoto N, Nakai-urakami C, Osawa Y, Tokunaga K, Sata T, Yaraamoto N, Sasazuki T, Ishi zaka Y.
  • Journal Title: Proc Natl Acad Sci U S A. 105 Pages: 7809-7814
  • Peer Reviewed
• [Presentation] 免疫抑制剤のSARSコロナウイルス増殖に与える影響についての解析 (2009)
  • Author(s): 原崎一浩、永田典代、岩田奈織子、長谷川秀樹、佐多徹太郎、山本直樹、高久 洋、佐藤人美、山本陽子、平松啓一、田代眞人、山本典生
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] Structure-based drug design(SBDD)によるSARSコロナウイルス増殖抑制薬剤の同定 (2009)
  • Author(s): 山本典生、永田典代、岩田奈織子、長谷川秀樹、佐多徹太郎、松本武久、高久 洋、山本陽子、佐藤人美、平松啓一、田代眞人、山本直樹
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] 免疫抑制剤のSARSコロナウイルス増殖に与える影響についての解析 (2009)
  • Author(s): 原崎一浩, 永田典代, 岩田奈織子, 長谷川秀樹, 佐多徹太郎, 山本直樹, 高久洋, 佐藤人美, 山本陽子, 平松啓一, 田代眞人, 山本典生
  • Organizer: 第57回日本ウイルス学会
  • Place of Presentation: 東京・都市センターホテル
  • Year and Date: 2009-10-25
• [Presentation] Structure-based drug design(SBDD)によるSARSコロナウイルス増殖抑制薬剤の同定 (2009)
  • Author(s): 山本典生, 永田典代, 岩田奈織子, 長谷川秀樹, 佐多徹太郎, 松本武久, 高久洋, 山本陽子, 佐藤人美, 平松啓一, 田代眞人, 山本直樹
  • Organizer: 第57回日本ウイルス学会
  • Place of Presentation: 東京・都市センターホテル
  • Year and Date: 2009-10-25
• [Presentation] ウイルス性呼吸器感染症に対する治療薬の開発を目指してインフルエンザと重症急性呼吸器症候群(SARS)～ (2009)
  • Author(s): 山本典生
  • Organizer: 第147回日本獣医学会学術集会シンポジウム(シンポジスト)
  • Place of Presentation: 栃木
  • Year and Date: 2009-04-03
• [Remarks] ホームページ等