Regulation of SARS-CoV replication by cyclophilin
Project/Area Number |
20590467
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Virology
|
Research Institution | Juntendo University |
Principal Investigator |
YAMAMOTO Norio Juntendo University, 医学部, 助教 (40323703)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
Keywords | SARSコロナウイルス / サイクロスポリンA / コロナウイルス / サイクロフィリン |
Research Abstract |
To investigate the relationship between cyclophilin and SARS-CoV replication, we introduced shRNA expression vectors to cells and compare the virus titer produced from these cells. Efficiency of SARS-CoV replication was much lower in the cells expressing shRNA against cyclophilin A and B than in the cells expressing control shRNA. The results of time-of-addition assay and entry assay showed that cyclosporin A inhibited SARS-CoV replication at the post-entry step in viral life cycle. These results suggested that cyclophilins could play a role as positive regulators of SARS-CoV replication at the post-entry step.
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Report
(4 results)
Research Products
(12 results)
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[Presentation] 免疫抑制剤のSARSコロナウイルス増殖に与える影響についての解析2009
Author(s)
原崎一浩, 永田典代, 岩田奈織子, 長谷川秀樹, 佐多徹太郎, 山本直樹, 高久洋, 佐藤人美, 山本陽子, 平松啓一, 田代眞人, 山本典生
Organizer
第57回日本ウイルス学会
Place of Presentation
東京・都市センターホテル
Year and Date
2009-10-25
Related Report
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[Presentation] Structure-based drug design(SBDD)によるSARSコロナウイルス増殖抑制薬剤の同定2009
Author(s)
山本典生, 永田典代, 岩田奈織子, 長谷川秀樹, 佐多徹太郎, 松本武久, 高久洋, 山本陽子, 佐藤人美, 平松啓一, 田代眞人, 山本直樹
Organizer
第57回日本ウイルス学会
Place of Presentation
東京・都市センターホテル
Year and Date
2009-10-25
Related Report
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