Comprehensive search and analysis of host factors that interact with the V protein of human parainfluenza virus type 2
| Project/Area Number |
20590469
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Mie University |
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Principal Investigator |
NISHIO Machiko Mie University, 大学院・医学系研究科, 講師 (70156040)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | パラインフルエンザ2型ウイルス / ウイルス増殖 / Yeast two-hybrid system / V蛋白 / P蛋白 / L蛋白 / 核移行シグナル(NLS) / 核外移行シグナル(NES) / hPIV2 / Yeast two-hybrid / 核移行シグナル / 核外移行シグナル / BLAST |
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| Research Abstract |
To search for host factors interacting with human parainfluenza virus type 2 (hPIV2) V protein systematically, I used the yeast two-hybrid system. About 1000 positive clones were isolated, sequenced, and identified about 100 proteins. The region for a complex with importin α, which is one of them, is important for gene expression. Moreover, I identified some proteins that interact with V protein, and increase in mRNA expression after V-minus rPIV2 infection by microarray data.
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Report
(4 results)
Research Products
(41 results)
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[Journal Article] Completion of the full-length genome sequence of human parainfluenza virus type 4A and 4B : Sequence analyses of the large protein genes, and gene start, intergenic and end sequences.2011
Author(s)
Komada H, Kawano M, Uefuji A, Ito M, Tsurudome M, Hatakeyama E, Nakanishi M, Sakue S, Job C, Suzumura E, Tamaki T, Tomioka T, Nishio M, Tsumura H, Uematsu J, Yamamoto H, O' Brien M, Bando H, Ito Y.
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Journal Title
Arch Virol. 156
Pages: 161-166
Related Report
Peer Reviewed
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[Journal Article] Completion of the full-length genome sequence of human parainfluenza virus type 4A and 4B : Sequence analyses of the large protein genes, and gene start, intergenic and end sequences.2011
Author(s)
Komada H, Kawano M, Uefuji A, Ito M, Tsurudome M, Hatakeyama E, Nakanishi M, Sakue S, Joh C, Suzumura E, Tamaki T, Tomioka T, Nishio M, Tsumura H, Uematsu J, Yamamoto H, O'Brien M, Bando H, Ito Y.
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Journal Title
Arch. Virol.
Volume: 156
Pages: 161-166
Related Report
Peer Reviewed
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