Comprehensive search and analysis of host factors that interact with the V protein of human parainfluenza virus type 2

• Project/Area Number: 20590469
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Mie University
• Principal Investigator: NISHIO Machiko Mie University, 大学院・医学系研究科, 講師 (70156040)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: パラインフルエンザ2型ウイルス / ウイルス増殖 / Yeast two-hybrid system / V蛋白 / P蛋白 / L蛋白 / 核移行シグナル(NLS) / 核外移行シグナル(NES) / hPIV2 / Yeast two-hybrid / 核移行シグナル / 核外移行シグナル / BLAST

Research Abstract

To search for host factors interacting with human parainfluenza virus type 2 (hPIV2) V protein systematically, I used the yeast two-hybrid system. About 1000 positive clones were isolated, sequenced, and identified about 100 proteins. The region for a complex with importin α, which is one of them, is important for gene expression. Moreover, I identified some proteins that interact with V protein, and increase in mRNA expression after V-minus rPIV2 infection by microarray data.

Research Products

• [Journal Article] A tryptophan-rich motif in the human parainfluenza virus type 2 V protein is critical for the blockade of Toll-like receptor 7/9 dependent signaling. (2011)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Takeuchi K, Itoh M, Gotoh B.
  • Journal Title: J.Virol. In press
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 2L protein regions required for interaction with other viral proteins and mRNA capping. (2011)
  • Author(s): Nishio M, Tsurudome M, Garcin D, Komada H, Ito M, Le Mercier P, Nosaka T, Kolakofsky D.
  • Journal Title: J.Virol. 85 Pages: 725-732
  • Peer Reviewed
• [Journal Article] Identification of domains on the fusion (F) protein trimer that influence the hemagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion. (2011)
  • Author(s): Tsurudome M, Tto M, Nishio M, Nakahashi, M Kawano M, Komada H, Nosaka T, Ito Y.
  • Journal Title: J.Virol. 85 Pages: 3153-3161
  • Peer Reviewed
• [Journal Article] Completion of the full-length genome sequence of human parainfluenza virus type 4A and 4B : Sequence analyses of the large protein genes, and gene start, intergenic and end sequences. (2011)
  • Author(s): Komada H, Kawano M, Uefuji A, Ito M, Tsurudome M, Hatakeyama E, Nakanishi M, Sakue S, Job C, Suzumura E, Tamaki T, Tomioka T, Nishio M, Tsumura H, Uematsu J, Yamamoto H, O' Brien M, Bando H, Ito Y.
  • Journal Title: Arch Virol. 156 Pages: 161-166
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 2 L protein regions required for interaction with other viral proteins and mRNA capping. (2011)
  • Author(s): Nishio M, Tsurudome M, Garcin D, Komada H, Ito M, Le Mercier P, Nosaka T, Kolakofsky D.
  • Journal Title: J.Virol. Volume: 85 Pages: 725-732
  • Peer Reviewed
• [Journal Article] Identification of domains on the fusion (F) protein trimer that influence the hamagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion. (2011)
  • Author(s): Tsurudome M, Ito M, Nishio M, Nakahashi M, Kawano M, Komada H, Nosaka T, Ito Y.
  • Journal Title: J.Virol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] A tryptophan-rich motif in the human parainfluenza virus type 2 V protein is critical for the blockade of Toll-like receptor 7/9 dependent signaling. (2011)
  • Author(s): Kitagawa Y, Yamaguchi M, Zhou M, Komatsu T, Nishio M, Sugiyama T, Takeuchi K, Itoh M, Gotoh B.
  • Journal Title: J.Virol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Completion of the full-length genome sequence of human parainfluenza virus type 4A and 4B : Sequence analyses of the large protein genes, and gene start, intergenic and end sequences. (2011)
  • Author(s): Komada H, Kawano M, Uefuji A, Ito M, Tsurudome M, Hatakeyama E, Nakanishi M, Sakue S, Joh C, Suzumura E, Tamaki T, Tomioka T, Nishio M, Tsumura H, Uematsu J, Yamamoto H, O'Brien M, Bando H, Ito Y.
  • Journal Title: Arch. Virol. Volume: 156 Pages: 161-166
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 2 V protein inhibits interferon production and signaling and is required for replication in non-human primates. (2010)
  • Author(s): Schaap-Nutt A, D' Angelo C, Scull M A, Amaro-Carambot E, Nishio M, Pickles R J, Collins P L, Murphy B R, Schmidt A C.
  • Journal Title: Virology 397 Pages: 285-298
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 2 V protein inhibits interferon production and signaling and is required for replication in non-human primates (2010)
  • Author(s): Schaap-Nutt A, D'Angelo C, Scull MA, Amaro-Carambot E, Nishio M, Pickles RJ, Collins PL, Murphy BR, Schmidt AC.
  • Journal Title: Virology 397 Pages: 285-298
  • Peer Reviewed
• [Journal Article] Effects of multiple amino acids of the parainfluenza virus 5 fusio protein on its haemagglutinin-neruaminidase-independ ent fusion activity. (2009)
  • Author(s): Ito M, Nishio M, Kawano M, Komada H, Ito Y, Tsurudome M.
  • Journal Title: J.Gen.Virol. 90 Pages: 405-413
  • Peer Reviewed
• [Journal Article] A quantitative method for analyzing establishing-effeciency of persistent viral infection. (2009)
  • Author(s): Ito M, Yamakawa I, Nishio M, Tsurudome M, Kawano M, Komada H, Ito Y.
  • Journal Title: Microbiol.Immunol. 53 Pages: 259-265
  • Peer Reviewed
• [Journal Article] A quantitative method for analyzing establishing-efficiency of persistent viral infection (2009)
  • Author(s): Ito M, Yamakawa I, Nishio M, Kawano M, Komada H, Ito Y.
  • Journal Title: Microbiol.Immunol. 53 Pages: 259-265
  • NAID: 10027686495
  • Peer Reviewed
• [Journal Article] Effects of multiple amino acids of the parainfluenza virus 5 fusion protein on haemagglutinin-neuraminidase-independent fusion activity. (2009)
  • Author(s): Morihiro Ito, et al.
  • Journal Title: J. Gen. Virol. 90 Pages: 405-413
  • Peer Reviewed
• [Journal Article] A Quantitative Method for Analyzing Establishing-Efficiency of Persistent Viral Infection. (2009)
  • Author(s): Morihiro Ito, et al.
  • Journal Title: Microbiol. Immunol. (In press)
  • Peer Reviewed
• [Journal Article] human parainfluenza virus type 2 V protein inhibits genome replication by binding to the L protein : Possible role in promoting viral fitness. (2008)
  • Author(s): Nishio M, Ohtsuka J, Tsurudome M, Nosaka T, Kolakofsky D.
  • Journal Title: J.Virol. 82 Pages: 6130-6138
  • Peer Reviewed
• [Journal Article] Effects of hemagglutinin-neuraminidase protein mutations on cell-cell fusion mediated by human parainfluenza type 2 virus. (2008)
  • Author(s): Tsurudome M, Nishio M, Ito M, Tanahashi S, Kawano M, Komada H, Ito Y.
  • Journal Title: J.Virol. 82 Pages: 8283-8295
  • Peer Reviewed
• [Journal Article] Human parainfluenza virus type 2 V protein inhibits genome replication by binding to the L protein : possible role in promotingviral fitness. (2008)
  • Author(s): Machiko Nishio, et al.
  • Journal Title: J. Virol. 82 Pages: 6130-6138
  • Peer Reviewed
• [Journal Article] Effects of hemagglutinin-neuraminidase protein mutations on cell-cell fusion mediated by human parainfluenza type 2 virus. (2008)
  • Author(s): Masato Tsurudome, et al.
  • Journal Title: J. Virol. 82 Pages: 8283-8295
  • Peer Reviewed
• [Presentation] ヒトパラインフルエンザ2型ウイルス(hPIV2)L蛋白の転写能に必須であるアミノ酸の同定 (2010)
  • Author(s): 西尾真智子、鶴留雅人、野阪哲哉
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] パラインフルエンザウイルスの膜融合誘導における受容体結合蛋白と膜融合蛋白の相互作用 (2010)
  • Author(s): 鶴留雅人、西尾真智子、伊藤守弘、河野光雄、野阪哲哉、駒田洋、伊藤康彦
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] パラミクソウイルスV蛋白はTRAF6のよるユビキチン化を阻害する (2010)
  • Author(s): 北川善紀、山口ゆみ、周敏、小松孝行、竹内健司、西尾真智子、伊藤正恵、後藤敏
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-08
• [Presentation] ヒトパラインフルエンザ2型ウイルス(hPIV2)L蛋白の転写能に必須であるアミノ酸の同定 (2010)
  • Author(s): 西尾真智子、鶴留雅人、野阪哲哉
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] パラインフルエンザウイルスの膜融合誘導における受容体結合蛋白と膜融合蛋白の相互作用 (2010)
  • Author(s): 鶴留雅人、西尾真智子、伊藤守弘、河野光雄、野阪哲哉、駒田洋、伊藤康彦
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] パラミクソウイルスV蛋白はTRAF6によるユビキチン化を阻害する (2010)
  • Author(s): 北川善紀、山口まゆ、周敏、小松孝行、竹内健司、西尾真智子、伊藤正恵、後藤敏
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: あわぎんホール(徳島)
  • Year and Date: 2010-11-08
• [Presentation] Identification of domains on the trimer surface of the fusion (F) protein that influence the hemagglutinin-neuraminidase specificity of the F protein in mediating cell-cell fusion. (2010)
  • Author(s): 鶴留雅人, 伊藤守弘, 西尾真智子
  • Organizer: XIV Internatinnal Conference on Negative Strand Viruses.
  • Place of Presentation: ブルージュ(ベルギー)
  • Year and Date: 2010-06-22
• [Presentation] Identification of domains on the trimer surface of the fusion (F) protein that influence the hemagglutinin-neuramiinidase specificity of the F protein in mediating cell-cell fusion. (2010)
  • Author(s): Tsurudome M, Ito M, Nishio M, Ito Y.
  • Organizer: XIV International Conference on Negative Strand Viruses
  • Place of Presentation: ブルージュ(ベルギー)
  • Year and Date: 2010-06-22
• [Presentation] Human parainfluenza virus type 2 L protein regions required for interaction with other viral proteins and polymerase activity. (2010)
  • Author(s): NIshio M, Tsurudome M, Ito M, Kolakofsky D.
  • Organizer: XIV International Conference on Negative Strand Viruses.
  • Place of Presentation: ブルージュ(ベルギー)
  • Year and Date: 2010-06-21
• [Presentation] Human parainfluenza virus type 2 L protein regions required for interaction with other viral proteins and polymerase activity. (2010)
  • Author(s): Nishio M, Tsurudome M, Ito M, Kolakofsky D.
  • Organizer: XIV International Conference on Negative Strand Viruses
  • Place of Presentation: ブルージュ(ベルギー)
  • Year and Date: 2010-06-21
• [Presentation] ヒトパラインフルエンザ2型ウイルス(hPIV2)P蛋白上の核移行シグナル(NLS)と核外移行シグナル(NES)の同定 (2009)
  • Author(s): 西尾真智子、大塚順平、鶴留雅人、野阪哲哉
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] ヒトパラインフルエンザ2型ウイルス (hPIV2) P蛋白上の核移行シグナル (NLS) と核外移行シグナル (NES) の同定 (2009)
  • Author(s): 西尾真智子, 大塚順平, 鶴留雅人, 野阪哲哉
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-27
• [Presentation] パラミクソウイルスの膜融合蛋白の解析:受容体結合蛋白との結合能と膜融合誘導能との関連性 (2009)
  • Author(s): 鶴留雅人、西尾真智子、伊藤守弘、河野光雄、駒田洋、伊藤康彦
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] パラミクソウイルスの膜融合蛋白の解析 : 受容体結合蛋白との結合能と膜融合誘導能との関連性 (2009)
  • Author(s): 鶴留雅人, 西尾真智子, 伊藤守弘, 河野光雄, 駒田洋, 伊藤康彦
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] パラインフルエンザ4型ウイルスの全遺伝子解析 (2008)
  • Author(s): 駒田洋、河野光雄、鶴留雅人、西尾真智子、伊藤守弘、伊藤康彦
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-10-27
• [Presentation] パラインフルエンザ4型ウイルスの全遺伝子解析 (2008)
  • Author(s): 駒田洋
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-27
• [Presentation] ヒトパラインフルエンザ2型ウイルス(hPIV2)L蛋白上のウイルス蛋白結合部位の同定とL蛋白の機能に必須である新たな領域の同定 (2008)
  • Author(s): 西尾真智子、駒田洋、鶴留雅人
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-10-26
• [Presentation] パラミクソウイルスの受容体結合蛋白(HN)による細胞融合(および細胞傷害)の制御機構 (2008)
  • Author(s): 鶴留雅人、西尾真智子、伊藤守弘、河野光雄、駒田洋、伊藤康彦
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-10-26
• [Presentation] ニパウイルスと局所感染型パラミクソウイルスのTIR7/9経路阻害能 (2008)
  • Author(s): 北川善紀、山口ゆみ、周敏、小松孝行、竹内健司、西尾真智子、伊藤正恵、後藤敏
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-10-26
• [Presentation] ヒトパラインフルェンザ2型ウイルス(hPTV2)L蛋白上のウイルス蛋白結合部位の同定とL蛋白の機能に必須である新たな領域の同定 (2008)
  • Author(s): 西尾真智子
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] パラミクソウイルスの受容体結合蛋白(HN)による細胞融合(および細胞傷害)の制御機構 (2008)
  • Author(s): 鶴留雅人
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26
• [Presentation] ニパウイルスと局所感染型パラミクソウイルスのTLR7/9経路阻害能 (2008)
  • Author(s): 北川善紀
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山県岡山市
  • Year and Date: 2008-10-26