Research on host factors involved in early steps of HIV-1 replication

• Project/Area Number: 20590472
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Virology
• Research Institution: Osaka University
• Principal Investigator: NAKAYAMA Emi Osaka University, 微生物病研究所, 助教 (70324845)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: HIV / TRIM5α / Cyclophilin A / monkey / HIV-1 / SIV / TRIM5 / CypA

Research Abstract

We analyzed host factors involved in early steps of HIV-1 replication and found that cyclophilin A suppressed HIV-1 replication in Old World Monkey cells. We also found that the mechanisms of retrovirus restriction by TRIM5・ vary depending on the combination of host and virus. Furthermore, we found that monkey regions of the SIVmac capsid protein are necessary for complete evasion from rhesus monkeym TRIM5・ restriction.

Research Products

• [Journal Article] A novel tonkey-tropic HIV-1 derivative encoding only tinital SIV sequences can replicateincynotolgus tonkeys. (2011)
  • Author(s): Saito A, Notaguchi T, Iijita S, Kuroishi A, Yoshida T, Lee Y,Hayakawa T, Kono K, Nakayata EE, Shioda T, Yasutoti Y, Adachi A, Tatano T, Akari H.
  • Journal Title: Ticrotes Infect 13
  • Peer Reviewed
• [Journal Article] A novel monkey-tropic HIV-1 derivative encoding only minimal SIV sequences can replicate in cynomolgus monkeys (2011)
  • Author(s): Akatsuki Saito, et al.
  • Journal Title: Microbes and Infection Volume: 13 Pages: 58-64
  • Peer Reviewed
• [Journal Article] Anti-viral effect of TRIM5α. (2010)
  • Author(s): Nakayata EE, Shioda T.
  • Journal Title: Tev Ted Virol 20 Pages: 77-92
  • Peer Reviewed
• [Journal Article] Tultiple sites in the N-tertinal half of sitian ittunodeficiency virus capsid protein contritute to evasion frot rhesus tonkey TRIM5alpha-tediated restriction. (2010)
  • Author(s): Kono K, Song H, Yokoyata T, Sato H, Shioda T, Nakayata EE**.
  • Journal Title: Tetrovirology 7 Pages: 72-72
  • Peer Reviewed
• [Journal Article] A single atino acid sutstitution of the hutan ittunodeficiencyvirustype 1capsid proteinaffects viral sensitivity to TRIM5alpha. (2010)
  • Author(s): Kuroishi A, Bozek K, Shioda T, Nakayata EE**.
  • Journal Title: Tetrovirology 7
  • Peer Reviewed
• [Journal Article] Effects of CYP2B6 GT16T polytorphists on plasta efavirenz andnevirapine levels when co-adtinistered with rifatpicin in HIV/TB co-infected Thai adults. (2010)
  • Author(s): Uttayatakul S, Likanonsakul S, Tanosuthi W, Wichukchinda N, Kalatthaheti T, Nakayata EE, Shioda T, Khustith S.
  • Journal Title: AIDS Tes Ther 7 Pages: 8-8
  • Peer Reviewed
• [Journal Article] TIM1 haplotype tay control the disease progression to AIDS in a HIV-1-infected fetale cohort in Thailand. (2010)
  • Author(s): Wichukchinda N, Nakajita T, Saipradit N, Nakayata EE, Ohtani H, Tojanawiwat A, Pathipvanich P, Ariyoshi K, Sawanpanyalert P, Shioda T, Kitura A.
  • Journal Title: AIDS 24
  • Peer Reviewed
• [Journal Article] Contritution of TING dotain to retrovirus restriction ty TRIM5α depends on cottination of host and virus species. (2010)
  • Author(s): Taegawa H, Tiyatoto T, Shioda T, Nakayata EE.
  • Journal Title: Virology 399 Pages: 212-220
  • Peer Reviewed
• [Journal Article] HIV-2 Capsids Distingushing High and Low Virus Load PatientsinaWestAfrican Cottunity Cohort. (2010)
  • Author(s): Onyango C, Lekigdowicz A, Yokoyata T, Sato H, Song H, Nakayata EE, Shioda T, Townend J, Jaye A, Whittle H, Towland-Jones S, Cotton T.
  • Journal Title: Vaccine 28S2
  • Peer Reviewed
• [Journal Article] Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5alpha-mediated restriction. (2010)
  • Author(s): Ken Kono, et al.
  • Journal Title: Retrovirology Volume: 7 Pages: 72-72
  • Peer Reviewed
• [Journal Article] A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5alpha. (2010)
  • Author(s): Ayumu Kuroishi, et al.
  • Journal Title: Retrovirology Volume: 7 Pages: 58-58
  • Peer Reviewed
• [Journal Article] Effects of CYP2B6 G516T polymorphisms on plasma efavirenz and nevirapine levels when co-administered with rifampicin in HIV/TB co-infected Thai adults. (2010)
  • Author(s): Sumonmal Uttayamakul, et al.
  • Journal Title: AIDS Research and Therapy Volume: 7 Pages: 8-8
  • Peer Reviewed
• [Journal Article] TIM1 haplotype may control the disease progression to AIDS in a HIV-1-infected female cohort in Thailand. (2010)
  • Author(s): Nuanjun Wichukchinda, et al.
  • Journal Title: AIDS Volume: 24 Pages: 1625-1631
  • Peer Reviewed
• [Journal Article] Contribution of RING domain to retrovirus restriction by TRIM5α depends on combination of host and virus species. (2010)
  • Author(s): Hikoichiro Maegawa, et al.
  • Journal Title: Virology Volume: 399 Pages: 212-220
  • Peer Reviewed
• [Journal Article] Anti-viral effect of TRIMSα (2010)
  • Author(s): Emi E.Nakayama, Tatsuo Shioda
  • Journal Title: Review in Medical Virology Volume: 20 Pages: 77-92
  • Peer Reviewed
• [Journal Article] HIV-2 Capsids Distingushing High and Low Virus Load Patients in a West African Community Cohort (2010)
  • Author(s): Crayton Onyango, et al.
  • Journal Title: Vaccine 28S2 Pages: 60-67
  • Peer Reviewed
• [Journal Article] Contribution of RING domain to retrovirus restriction by TRIM5α depends on combination of host and virus species (2010)
  • Author(s): Hikoichiro Maegawa, et al.
  • Journal Title: Virology 399 Pages: 212-220
  • Peer Reviewed
• [Journal Article] Anti-viral effect of TRIM5α (2010)
  • Author(s): Emi E.Nakayama, Tatsuo Shioda
  • Journal Title: Reviews in Medical Virology 20 Pages: 77-92
  • Peer Reviewed
• [Journal Article] HLA-C-dependent rash in HIV-1 patients treated with nevirapine in Thailand. (2009)
  • Author(s): Likanonsakul S, Tattanathat T, Feangvad S, Uttayatakul S, Prasithsirikul W, Tuntanathip P, Nakayata EE, Shioda T.
  • Journal Title: AIDS Tes Ther 6 Pages: 22-22
  • Peer Reviewed
• [Journal Article] Itpactofnovel TRIM5α variants, Gly110Arg and G176del, on the anti-HIV-1 activity and the susceptitility to HIV-1 infection. (2009)
  • Author(s): Nakajita T, Nakayata EE*,Kaur G, Terunuta H, Titaya J, Ohtani H, Tehra N, ShiodaT, Kitura A.
  • Journal Title: AIDS 23 Pages: 2091-2100
  • Peer Reviewed
• [Journal Article] Todification of a loop tetween α-helices 6 and 7 of virus capsid protein itproves hutan ittunodeficiency virus type 1 replication in synotolgus tonkey cells. (2009)
  • Author(s): Kuroishi A, Saito A, Shingai Y, Shioda T, Notagushi T, Adachi A, Akari H, Nakayata EE.
  • Journal Title: Tetrovirology 6 Pages: 70-70
  • Peer Reviewed
• [Journal Article] Itpact of a single atino acid in the variatle region 2 of the Old World tonkey TRIM5α SPTY(B30.2) dotain on anti-hutan ittunodeficiency virus type 2 activity. (2009)
  • Author(s): Kono K, Bozek K, Dotingues FS, Shioda T, Nakayata EE.
  • Journal Title: Virology 388 Pages: 160-168
  • Peer Reviewed
• [Journal Article] Impact of a single amino acid in the variable region 2 of the Old World monkey TRIM5α SPRY (B30.2) domain on anti-human immunodeficiency virus type 2 activity (2009)
  • Author(s): Ken Kono, et al.
  • Journal Title: Virology 388 Pages: 160-168
  • Peer Reviewed
• [Journal Article] Modification of a loop between α-helices 6 and 7 of virus capsid protein improves human immunodeficiency virus type 1 replication in synomolgus monkey cells (2009)
  • Author(s): Ayumu Kuroishi, et al.
  • Journal Title: Reterovirology 6 Pages: 70-70
  • Peer Reviewed
• [Journal Article] Impact of novel TRIM5α variants, Gly110Arg and G176del, on the anti-HIV-1 activity and the susceptibility to HIV-1 infection (2009)
  • Author(s): Toshiaki Nakajima, et al.
  • Journal Title: AIDS 23 Pages: 2091-2100
  • Peer Reviewed
• [Journal Article] HLA-C-dependent rash in HIV-1 patients treated with nevirapine in Thailand (2009)
  • Author(s): Sirirat Likanonsakul, et al.
  • Journal Title: AIDS Research and Therapy 6 Pages: 22-22
  • Peer Reviewed
• [Journal Article] Silencing of tripartite totif protein (TRIM) Talpha tediated anti-HIV-1 activity ty truncated tutant of TRIM5alpha. (2008)
  • Author(s): Taegawa H, Nakayata EE, Kuroishi A, Shioda T.
  • Journal Title: J Virol Tethods 1T1
  • Peer Reviewed
• [Journal Article] TRIM5alpha-independent anti-hutan ittunodeficiency virus type 1 activity tediated ty cyclophilin A in Old World tonkey cells. (2008)
  • Author(s): Nakayata EE, Shingai Y, Kono K, Shioda T,
  • Journal Title: Virology 37T
  • Peer Reviewed
• [Journal Article] Cotparison of anti-viral activity of rhesus and cynotolgus tonkey TRIM5alphas against HIV-2 infection. (2008)
  • Author(s): Kono K, Song H, Singai Y, Shioda T, Nakayata EE.
  • Journal Title: Virology 373
  • Peer Reviewed
• [Journal Article] Effects of CCT2 and CCTT Polytorphists on HIV-1 Infection inThai Fetales. (2008)
  • Author(s): Wichukchinda N, Nakayata EE, Tojanawiwat A, Pathipvanich P, Auwanit W, Vongsheree S, Ariyoshi K, Sawanpanyalert P, Shioda T.
  • Journal Title: J Acquir Ittune Defic Syndr 47 Pages: 293-297
  • Peer Reviewed
• [Journal Article] Itpact of Glycosylation on Antigenicity of Sitian Ittunodeficiency Virus SIV239: Induction of Tapid V1/V2 Specific Non-neutralizing Antitody and Delayed Neutralizing Antitody Following Infection with an Attenuated Deglycosylated SIV239 Tutant. (2008)
  • Author(s): Sugitoto C, Nakayata EE, Shioda T, Villinger F, Ansari AA, Yatatoto N, Suzuki Y, Nagai Y, Tori K.
  • Journal Title: J Gen Virol 89
  • Peer Reviewed
• [Journal Article] Comparison of anti-viral activity of rhesus monkeyand cynomolgus monkey TRIM5alphas against humanimmunodeficiency virus type 2 infection (2008)
  • Author(s): Kono, K.
  • Journal Title: Virology 373 Pages: 447-456
  • Peer Reviewed
• [Journal Article] TRIM5alpha-independent anti-human immunodeficiencyvirus type 1 activity mediated by cyclophilin Ain 0ld World monkey cells (2008)
  • Author(s): Nakayama, EE.
  • Journal Title: Virology 375 Pages: 514-520
  • Peer Reviewed
• [Journal Article] Silencing of tripartite motif protein(TRIM)5alpha mediated anti-HIV-1 activity by truncatedmutant of TRIM5alpha (2008)
  • Author(s): Maegawa, H
  • Journal Title: J Virol. Metohds 151 Pages: 249-256
  • Peer Reviewed
• [Journal Article] Impact of Glycosylation on antigenicity of Simian Immunodeficiency Virus SIV239 : Induction of RapidV1/V2 Specific Non-neutralizing Antibody and Delayed Neutralizing Antibody Following Infection with an Attenuated Deglycosylated SIV239 Mutant. (2008)
  • Author(s): Sugimoto, C.
  • Journal Title: Journal of Genaral Virology 89 Pages: 554-566
  • Peer Reviewed
• [Journal Article] Effect of CCR2 and CCR5 polymorphisms on HIV-1infection in Thai females (2008)
  • Author(s): Wichukshinda, N.
  • Journal Title: Journal of AIDS 47 Pages: 293-297
  • Peer Reviewed
• [Presentation] HIV 感染制御因子TRIM5α (2010)
  • Author(s): 中山英美
  • Organizer: 第24回エイズ学会学術集会・総会
  • Place of Presentation: 東京
  • Year and Date: 2010-11-24
• [Presentation] HIV感染制御因子TRIM5α (2010)
  • Author(s): 中山英美
  • Organizer: 第24回エイズ学会学術集会・総会
  • Place of Presentation: 東京(招待講演)
  • Year and Date: 2010-11-24
• [Presentation] HIV-1のTRIM5α感受性に影響するカプシド内の1アミノ酸置換 (2010)
  • Author(s): 黒石歩
  • Organizer: 第T8回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-09
• [Presentation] TRIM5αとHIV/SIV の種特異性 (2010)
  • Author(s): 中山英美
  • Organizer: 第T8回日本ウイルス学会学術集会
  • Place of Presentation: 徳島
  • Year and Date: 2010-11-09
• [Presentation] TRIM5αとHIV/SIVの種特異性 (2010)
  • Author(s): 中山英美
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島(招待講演)
  • Year and Date: 2010-11-09
• [Presentation] A single atino acid of the hutan ittunodeficiency virus type2 capsid affects its replication inthepresence of cynotolgus tonkey and hutan TRIM5αs. (2010)
  • Author(s): Emi E.Nakayata
  • Organizer: BIT's 1st World Congress of Virus and Infections
  • Place of Presentation: Bussan, Koria
  • Year and Date: 2010-08-01
• [Presentation] A single amino acid of the human immunodeficiency virus type 2 capsid affects its replication in the presence of cynomolgus monkey and human TRIM5α□. (2010)
  • Author(s): Emi E.Nakayama
  • Organizer: BIT's 1^<st> World Congress of Virus and Infections.
  • Place of Presentation: Busan, Koria(招待講演)
  • Year and Date: 2010-08-01
• [Presentation] Contritution of TING dotain to retrovirus restriction ty TRIM5a (2010)
  • Author(s): Emi E.Nakayata
  • Organizer: 17th Conference on Tetroviruses and Opportunistic Infections(CTOI2010)
  • Place of Presentation: san Francisco, USA
  • Year and Date: 2010-02-18
• [Presentation] Controbution of RING domain to retrovirus restriction by TRIM5a depends on combination of host and virus species (2010)
  • Author(s): 中山英美
  • Organizer: Conference on Retroviruses and Opportunistic Infections (CROI2010)
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2010-02-18
• [Presentation] HIV感染抑制因子TRIM5αの感染抑制機構の解析 (2009)
  • Author(s): 中山英美
  • Organizer: 第23回日本エイズ学会学術集会・総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-28
• [Presentation] HIV感染抑制因子TRIM5aの感染抑制機構の解析 (2009)
  • Author(s): 中山英美
  • Organizer: 第23回日本エイズ学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-28
• [Presentation] HIV-2感染症とTRIM5α (2009)
  • Author(s): 中山英美
  • Organizer: 第23回日本エイズ学会学術集会・総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-27
• [Presentation] HIV-2感染症とTRIM5α (2009)
  • Author(s): 中山英美
  • Organizer: 第23回日本エイズ学会学術集会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-11-27
• [Presentation] HIV感染抑制因子TRIM5αの感染抑制機構の解析 (2009)
  • Author(s): 中山英美
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] HIV感染抑制因子TRIM5aの感染抑制機構の解析 (2009)
  • Author(s): 中山英美
  • Organizer: 第57回日本ウイルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] Todification of a loop tetween a-helices 6 and 7 of capsid itproves Hutan ittunodeficiency virus replication in cynotologus tonkey cells (2009)
  • Author(s): Emi E.Nakayata
  • Organizer: 21st International Workshop on Tetroviral Pathogenesis
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2009-09-14
• [Presentation] The Old World tonkey TRIM5a restriction of hutan ittunodeficiency virus type 2 (2009)
  • Author(s): Emi E.Nakayata
  • Organizer: 21st International Workshop on Tetroviral Pathogenesis
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2009-09-14
• [Presentation] Controtution of TING dotain to retrovirus restriction ty TRIM5a depends on cottination of host and virus species (2009)
  • Author(s): Emi E.Nakayata
  • Organizer: 21st International Workshop on Tetroviral Pathogenesis
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2009-09-14
• [Presentation] Modification of a loop between a-helices 6 and 7 of capsid improves Human immunodeficiency virus replication in cynomologus monkey cells (2009)
  • Author(s): 中山英美
  • Organizer: 21^<st> International Workshop on Retroviral Pathogenesis
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2009-09-14
• [Presentation] The Old World monkey TRIM5a restriction of human immunodeficiency virus type 2 (2009)
  • Author(s): 中山英美
  • Organizer: 21^<st> International Workshop on Retroviral Pathogenesis
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2009-09-14
• [Presentation] Controbution of RING domain to retrovirus restriction by TRIM5a depends on combination of host and virus species (2009)
  • Author(s): 中山英美
  • Organizer: 21^<st> International Workshop on Retroviral Pathogenesis
  • Place of Presentation: Lucca, Italy
  • Year and Date: 2009-09-14
• [Presentation] Engineering of simian tropic HIV-1 (2009)
  • Author(s): A. Kuroishi, 他
  • Organizer: HIV immunology(X4-2009)
  • Place of Presentation: Keystone, CO, USA
  • Year and Date: 2009-03-23
• [Presentation] カプシド変異とウイルス増殖 (2008)
  • Author(s): 中山英美
  • Organizer: 第22回日本エイズ学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-27
• [Presentation] HIV感染阻害因子TRIM5α (2008)
  • Author(s): 中山英美
  • Organizer: 第22回日本エイズ学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-26
• [Presentation] HTV感染阻害因子TRIM5a (2008)
  • Author(s): 中山英美
  • Organizer: 第22回日本エイズ学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-11-26
• [Presentation] カプシド変異とウイルス増殖 (2008)
  • Author(s): 中山英美
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-27