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Research on host factors involved in early steps of HIV-1 replication

Research Project

Project/Area Number 20590472
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Virology
Research InstitutionOsaka University

Principal Investigator

NAKAYAMA Emi  Osaka University, 微生物病研究所, 助教 (70324845)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsHIV / TRIM5α / Cyclophilin A / monkey / HIV-1 / SIV / TRIM5 / CypA
Research Abstract

We analyzed host factors involved in early steps of HIV-1 replication and found that cyclophilin A suppressed HIV-1 replication in Old World Monkey cells. We also found that the mechanisms of retrovirus restriction by TRIM5・ vary depending on the combination of host and virus. Furthermore, we found that monkey regions of the SIVmac capsid protein are necessary for complete evasion from rhesus monkeym TRIM5・ restriction.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (62 results)

All 2011 2010 2009 2008

All Journal Article (36 results) (of which Peer Reviewed: 36 results) Presentation (26 results)

  • [Journal Article] A novel tonkey-tropic HIV-1 derivative encoding only tinital SIV sequences can replicateincynotolgus tonkeys.2011

    • Author(s)
      Saito A, Notaguchi T, Iijita S, Kuroishi A, Yoshida T, Lee Y,Hayakawa T, Kono K, Nakayata EE, Shioda T, Yasutoti Y, Adachi A, Tatano T, Akari H.
    • Journal Title

      Ticrotes Infect 13

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel monkey-tropic HIV-1 derivative encoding only minimal SIV sequences can replicate in cynomolgus monkeys2011

    • Author(s)
      Akatsuki Saito, et al.
    • Journal Title

      Microbes and Infection

      Volume: 13 Pages: 58-64

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-viral effect of TRIM5α.2010

    • Author(s)
      Nakayata EE, Shioda T.
    • Journal Title

      Tev Ted Virol 20

      Pages: 77-92

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Tultiple sites in the N-tertinal half of sitian ittunodeficiency virus capsid protein contritute to evasion frot rhesus tonkey TRIM5alpha-tediated restriction.2010

    • Author(s)
      Kono K, Song H, Yokoyata T, Sato H, Shioda T, Nakayata EE**.
    • Journal Title

      Tetrovirology 7

      Pages: 72-72

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A single atino acid sutstitution of the hutan ittunodeficiencyvirustype 1capsid proteinaffects viral sensitivity to TRIM5alpha.2010

    • Author(s)
      Kuroishi A, Bozek K, Shioda T, Nakayata EE**.
    • Journal Title

      Tetrovirology 7

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of CYP2B6 GT16T polytorphists on plasta efavirenz andnevirapine levels when co-adtinistered with rifatpicin in HIV/TB co-infected Thai adults.2010

    • Author(s)
      Uttayatakul S, Likanonsakul S, Tanosuthi W, Wichukchinda N, Kalatthaheti T, Nakayata EE, Shioda T, Khustith S.
    • Journal Title

      AIDS Tes Ther 7

      Pages: 8-8

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TIM1 haplotype tay control the disease progression to AIDS in a HIV-1-infected fetale cohort in Thailand.2010

    • Author(s)
      Wichukchinda N, Nakajita T, Saipradit N, Nakayata EE, Ohtani H, Tojanawiwat A, Pathipvanich P, Ariyoshi K, Sawanpanyalert P, Shioda T, Kitura A.
    • Journal Title

      AIDS 24

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contritution of TING dotain to retrovirus restriction ty TRIM5α depends on cottination of host and virus species.2010

    • Author(s)
      Taegawa H, Tiyatoto T, Shioda T, Nakayata EE.
    • Journal Title

      Virology 399

      Pages: 212-220

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] HIV-2 Capsids Distingushing High and Low Virus Load PatientsinaWestAfrican Cottunity Cohort.2010

    • Author(s)
      Onyango C, Lekigdowicz A, Yokoyata T, Sato H, Song H, Nakayata EE, Shioda T, Townend J, Jaye A, Whittle H, Towland-Jones S, Cotton T.
    • Journal Title

      Vaccine 28S2

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Multiple sites in the N-terminal half of simian immunodeficiency virus capsid protein contribute to evasion from rhesus monkey TRIM5alpha-mediated restriction.2010

    • Author(s)
      Ken Kono, et al.
    • Journal Title

      Retrovirology

      Volume: 7 Pages: 72-72

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A single amino acid substitution of the human immunodeficiency virus type 1 capsid protein affects viral sensitivity to TRIM5alpha.2010

    • Author(s)
      Ayumu Kuroishi, et al.
    • Journal Title

      Retrovirology

      Volume: 7 Pages: 58-58

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of CYP2B6 G516T polymorphisms on plasma efavirenz and nevirapine levels when co-administered with rifampicin in HIV/TB co-infected Thai adults.2010

    • Author(s)
      Sumonmal Uttayamakul, et al.
    • Journal Title

      AIDS Research and Therapy

      Volume: 7 Pages: 8-8

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TIM1 haplotype may control the disease progression to AIDS in a HIV-1-infected female cohort in Thailand.2010

    • Author(s)
      Nuanjun Wichukchinda, et al.
    • Journal Title

      AIDS

      Volume: 24 Pages: 1625-1631

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of RING domain to retrovirus restriction by TRIM5α depends on combination of host and virus species.2010

    • Author(s)
      Hikoichiro Maegawa, et al.
    • Journal Title

      Virology

      Volume: 399 Pages: 212-220

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-viral effect of TRIMSα2010

    • Author(s)
      Emi E.Nakayama, Tatsuo Shioda
    • Journal Title

      Review in Medical Virology

      Volume: 20 Pages: 77-92

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HIV-2 Capsids Distingushing High and Low Virus Load Patients in a West African Community Cohort2010

    • Author(s)
      Crayton Onyango, et al.
    • Journal Title

      Vaccine 28S2

      Pages: 60-67

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of RING domain to retrovirus restriction by TRIM5α depends on combination of host and virus species2010

    • Author(s)
      Hikoichiro Maegawa, et al.
    • Journal Title

      Virology 399

      Pages: 212-220

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anti-viral effect of TRIM5α2010

    • Author(s)
      Emi E.Nakayama, Tatsuo Shioda
    • Journal Title

      Reviews in Medical Virology 20

      Pages: 77-92

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HLA-C-dependent rash in HIV-1 patients treated with nevirapine in Thailand.2009

    • Author(s)
      Likanonsakul S, Tattanathat T, Feangvad S, Uttayatakul S, Prasithsirikul W, Tuntanathip P, Nakayata EE, Shioda T.
    • Journal Title

      AIDS Tes Ther 6

      Pages: 22-22

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Itpactofnovel TRIM5α variants, Gly110Arg and G176del, on the anti-HIV-1 activity and the susceptitility to HIV-1 infection.2009

    • Author(s)
      Nakajita T, Nakayata EE*,Kaur G, Terunuta H, Titaya J, Ohtani H, Tehra N, ShiodaT, Kitura A.
    • Journal Title

      AIDS 23

      Pages: 2091-2100

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Todification of a loop tetween α-helices 6 and 7 of virus capsid protein itproves hutan ittunodeficiency virus type 1 replication in synotolgus tonkey cells.2009

    • Author(s)
      Kuroishi A, Saito A, Shingai Y, Shioda T, Notagushi T, Adachi A, Akari H, Nakayata EE.
    • Journal Title

      Tetrovirology 6

      Pages: 70-70

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Itpact of a single atino acid in the variatle region 2 of the Old World tonkey TRIM5α SPTY(B30.2) dotain on anti-hutan ittunodeficiency virus type 2 activity.2009

    • Author(s)
      Kono K, Bozek K, Dotingues FS, Shioda T, Nakayata EE.
    • Journal Title

      Virology 388

      Pages: 160-168

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impact of a single amino acid in the variable region 2 of the Old World monkey TRIM5α SPRY (B30.2) domain on anti-human immunodeficiency virus type 2 activity2009

    • Author(s)
      Ken Kono, et al.
    • Journal Title

      Virology 388

      Pages: 160-168

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modification of a loop between α-helices 6 and 7 of virus capsid protein improves human immunodeficiency virus type 1 replication in synomolgus monkey cells2009

    • Author(s)
      Ayumu Kuroishi, et al.
    • Journal Title

      Reterovirology 6

      Pages: 70-70

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of novel TRIM5α variants, Gly110Arg and G176del, on the anti-HIV-1 activity and the susceptibility to HIV-1 infection2009

    • Author(s)
      Toshiaki Nakajima, et al.
    • Journal Title

      AIDS 23

      Pages: 2091-2100

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HLA-C-dependent rash in HIV-1 patients treated with nevirapine in Thailand2009

    • Author(s)
      Sirirat Likanonsakul, et al.
    • Journal Title

      AIDS Research and Therapy 6

      Pages: 22-22

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silencing of tripartite totif protein (TRIM) Talpha tediated anti-HIV-1 activity ty truncated tutant of TRIM5alpha.2008

    • Author(s)
      Taegawa H, Nakayata EE, Kuroishi A, Shioda T.
    • Journal Title

      J Virol Tethods 1T1

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] TRIM5alpha-independent anti-hutan ittunodeficiency virus type 1 activity tediated ty cyclophilin A in Old World tonkey cells.2008

    • Author(s)
      Nakayata EE, Shingai Y, Kono K, Shioda T,
    • Journal Title

      Virology 37T

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cotparison of anti-viral activity of rhesus and cynotolgus tonkey TRIM5alphas against HIV-2 infection.2008

    • Author(s)
      Kono K, Song H, Singai Y, Shioda T, Nakayata EE.
    • Journal Title

      Virology 373

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of CCT2 and CCTT Polytorphists on HIV-1 Infection inThai Fetales.2008

    • Author(s)
      Wichukchinda N, Nakayata EE, Tojanawiwat A, Pathipvanich P, Auwanit W, Vongsheree S, Ariyoshi K, Sawanpanyalert P, Shioda T.
    • Journal Title

      J Acquir Ittune Defic Syndr 47

      Pages: 293-297

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Itpact of Glycosylation on Antigenicity of Sitian Ittunodeficiency Virus SIV239: Induction of Tapid V1/V2 Specific Non-neutralizing Antitody and Delayed Neutralizing Antitody Following Infection with an Attenuated Deglycosylated SIV239 Tutant.2008

    • Author(s)
      Sugitoto C, Nakayata EE, Shioda T, Villinger F, Ansari AA, Yatatoto N, Suzuki Y, Nagai Y, Tori K.
    • Journal Title

      J Gen Virol 89

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of anti-viral activity of rhesus monkeyand cynomolgus monkey TRIM5alphas against humanimmunodeficiency virus type 2 infection2008

    • Author(s)
      Kono, K.
    • Journal Title

      Virology 373

      Pages: 447-456

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TRIM5alpha-independent anti-human immunodeficiencyvirus type 1 activity mediated by cyclophilin Ain 0ld World monkey cells2008

    • Author(s)
      Nakayama, EE.
    • Journal Title

      Virology 375

      Pages: 514-520

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Silencing of tripartite motif protein(TRIM)5alpha mediated anti-HIV-1 activity by truncatedmutant of TRIM5alpha2008

    • Author(s)
      Maegawa, H
    • Journal Title

      J Virol. Metohds 151

      Pages: 249-256

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impact of Glycosylation on antigenicity of Simian Immunodeficiency Virus SIV239 : Induction of RapidV1/V2 Specific Non-neutralizing Antibody and Delayed Neutralizing Antibody Following Infection with an Attenuated Deglycosylated SIV239 Mutant.2008

    • Author(s)
      Sugimoto, C.
    • Journal Title

      Journal of Genaral Virology 89

      Pages: 554-566

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of CCR2 and CCR5 polymorphisms on HIV-1infection in Thai females2008

    • Author(s)
      Wichukshinda, N.
    • Journal Title

      Journal of AIDS 47

      Pages: 293-297

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] HIV 感染制御因子TRIM5α2010

    • Author(s)
      中山英美
    • Organizer
      第24回エイズ学会学術集会・総会
    • Place of Presentation
      東京
    • Year and Date
      2010-11-24
    • Related Report
      2010 Final Research Report
  • [Presentation] HIV感染制御因子TRIM5α2010

    • Author(s)
      中山英美
    • Organizer
      第24回エイズ学会学術集会・総会
    • Place of Presentation
      東京(招待講演)
    • Year and Date
      2010-11-24
    • Related Report
      2010 Annual Research Report
  • [Presentation] HIV-1のTRIM5α感受性に影響するカプシド内の1アミノ酸置換2010

    • Author(s)
      黒石歩
    • Organizer
      第T8回日本ウイルス学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-09
    • Related Report
      2010 Final Research Report
  • [Presentation] TRIM5αとHIV/SIV の種特異性2010

    • Author(s)
      中山英美
    • Organizer
      第T8回日本ウイルス学会学術集会
    • Place of Presentation
      徳島
    • Year and Date
      2010-11-09
    • Related Report
      2010 Final Research Report
  • [Presentation] TRIM5αとHIV/SIVの種特異性2010

    • Author(s)
      中山英美
    • Organizer
      第58回日本ウイルス学会学術集会
    • Place of Presentation
      徳島(招待講演)
    • Year and Date
      2010-11-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] A single atino acid of the hutan ittunodeficiency virus type2 capsid affects its replication inthepresence of cynotolgus tonkey and hutan TRIM5αs.2010

    • Author(s)
      Emi E.Nakayata
    • Organizer
      BIT's 1st World Congress of Virus and Infections
    • Place of Presentation
      Bussan, Koria
    • Year and Date
      2010-08-01
    • Related Report
      2010 Final Research Report
  • [Presentation] A single amino acid of the human immunodeficiency virus type 2 capsid affects its replication in the presence of cynomolgus monkey and human TRIM5α□.2010

    • Author(s)
      Emi E.Nakayama
    • Organizer
      BIT's 1^<st> World Congress of Virus and Infections.
    • Place of Presentation
      Busan, Koria(招待講演)
    • Year and Date
      2010-08-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Contritution of TING dotain to retrovirus restriction ty TRIM5a2010

    • Author(s)
      Emi E.Nakayata
    • Organizer
      17th Conference on Tetroviruses and Opportunistic Infections(CTOI2010)
    • Place of Presentation
      san Francisco, USA
    • Year and Date
      2010-02-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Controbution of RING domain to retrovirus restriction by TRIM5a depends on combination of host and virus species2010

    • Author(s)
      中山英美
    • Organizer
      Conference on Retroviruses and Opportunistic Infections (CROI2010)
    • Place of Presentation
      San Francisco, USA
    • Year and Date
      2010-02-18
    • Related Report
      2009 Annual Research Report
  • [Presentation] HIV感染抑制因子TRIM5αの感染抑制機構の解析2009

    • Author(s)
      中山英美
    • Organizer
      第23回日本エイズ学会学術集会・総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-11-28
    • Related Report
      2010 Final Research Report
  • [Presentation] HIV感染抑制因子TRIM5aの感染抑制機構の解析2009

    • Author(s)
      中山英美
    • Organizer
      第23回日本エイズ学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-11-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] HIV-2感染症とTRIM5α2009

    • Author(s)
      中山英美
    • Organizer
      第23回日本エイズ学会学術集会・総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-11-27
    • Related Report
      2010 Final Research Report
  • [Presentation] HIV-2感染症とTRIM5α2009

    • Author(s)
      中山英美
    • Organizer
      第23回日本エイズ学会学術集会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-11-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] HIV感染抑制因子TRIM5αの感染抑制機構の解析2009

    • Author(s)
      中山英美
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-25
    • Related Report
      2010 Final Research Report
  • [Presentation] HIV感染抑制因子TRIM5aの感染抑制機構の解析2009

    • Author(s)
      中山英美
    • Organizer
      第57回日本ウイルス学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2009-10-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Todification of a loop tetween a-helices 6 and 7 of capsid itproves Hutan ittunodeficiency virus replication in cynotologus tonkey cells2009

    • Author(s)
      Emi E.Nakayata
    • Organizer
      21st International Workshop on Tetroviral Pathogenesis
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2009-09-14
    • Related Report
      2010 Final Research Report
  • [Presentation] The Old World tonkey TRIM5a restriction of hutan ittunodeficiency virus type 22009

    • Author(s)
      Emi E.Nakayata
    • Organizer
      21st International Workshop on Tetroviral Pathogenesis
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2009-09-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Controtution of TING dotain to retrovirus restriction ty TRIM5a depends on cottination of host and virus species2009

    • Author(s)
      Emi E.Nakayata
    • Organizer
      21st International Workshop on Tetroviral Pathogenesis
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2009-09-14
    • Related Report
      2010 Final Research Report
  • [Presentation] Modification of a loop between a-helices 6 and 7 of capsid improves Human immunodeficiency virus replication in cynomologus monkey cells2009

    • Author(s)
      中山英美
    • Organizer
      21^<st> International Workshop on Retroviral Pathogenesis
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] The Old World monkey TRIM5a restriction of human immunodeficiency virus type 22009

    • Author(s)
      中山英美
    • Organizer
      21^<st> International Workshop on Retroviral Pathogenesis
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Controbution of RING domain to retrovirus restriction by TRIM5a depends on combination of host and virus species2009

    • Author(s)
      中山英美
    • Organizer
      21^<st> International Workshop on Retroviral Pathogenesis
    • Place of Presentation
      Lucca, Italy
    • Year and Date
      2009-09-14
    • Related Report
      2009 Annual Research Report
  • [Presentation] Engineering of simian tropic HIV-12009

    • Author(s)
      A. Kuroishi, 他
    • Organizer
      HIV immunology(X4-2009)
    • Place of Presentation
      Keystone, CO, USA
    • Year and Date
      2009-03-23
    • Related Report
      2008 Annual Research Report
  • [Presentation] カプシド変異とウイルス増殖2008

    • Author(s)
      中山英美
    • Organizer
      第22回日本エイズ学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2008-11-27
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] HIV感染阻害因子TRIM5α2008

    • Author(s)
      中山英美
    • Organizer
      第22回日本エイズ学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2008-11-26
    • Related Report
      2010 Final Research Report
  • [Presentation] HTV感染阻害因子TRIM5a2008

    • Author(s)
      中山英美
    • Organizer
      第22回日本エイズ学会学術集会
    • Place of Presentation
      大阪
    • Year and Date
      2008-11-26
    • Related Report
      2008 Annual Research Report
  • [Presentation] カプシド変異とウイルス増殖2008

    • Author(s)
      中山英美
    • Organizer
      第56回日本ウイルス学会学術集会
    • Place of Presentation
      岡山
    • Year and Date
      2008-10-27
    • Related Report
      2010 Final Research Report 2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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