| Project/Area Number |
20590478
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Virology
|
|---|
| Research Institution | Chubu University |
|---|
Principal Investigator |
ITO Yasuhiko Chubu University, 生命健康科学部, 教授 (00022872)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
ITO Morihiro 中部大学, 生命健康科学部, 准教授 (10281081)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | パラミクソウイルス / 持続感染 / センダイウイルス / 細胞傷害 / インターフェロン / 持続感染細胞 / HN蛋白 / F蛋白 / パラインフルエンザイルス / 定量法 / steady state / パラインフルエンザウイルス |
|---|
| Research Abstract |
We tried to devise a quantitative method for analyzing establishing-efficiency of persistent viral infection. Subsequently, we analyzed factors of Sendai virus contributing to persistent infection by applying the quantitative method, indicating that L protein plays a determinative role in establishment of and M protein has an additional ability to induce persistent infection Fpi protein also contributes to persistent viral infection, but HNpi protein shows suppressive effect on establishment of persistent viral infection. HPIV2 induced cell toxicity, that is, giant cell formation, was suppressed by Rho kinase inhibitor, indicating that Rho kinase plays a positive role in HPIV2 induced cell toxicity and C kinase inhibitor enhanced HPIV2 induced cell toxicity. Intriguingly, the protein kinases showed intracellular networks.
|
