The analysis of immune responses in humanized NOG mice

• Project/Area Number: 20590484
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Tohoku University
• Principal Investigator: TAKAHASHI Takeshi Tohoku University, 大学院・医学系研究科, 助教 (80335215)
• Co-Investigator(Kenkyū-buntansha): MURATA Kazuko いわき明星大学, 薬学部, 教授 (20137631)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: リンパ球 / ヒト化マウス / HLA-DR / T細胞 / NOGマウス

Research Abstract

The immunological features of the human B and T lymphocyteswere analyzed, which are reconstituted in super-immunodeficient NOG mice. The mature IgD+ B cells produced IgG in response to antigen and cytokine stimulation in vitro. The T cells in the periphery in the humanized NOG mice could neither proliferate nor produce IL-2 in response to antigenic stimulation. The problem is associated with the reduction of the frequency of naive T cells and the increase of that of effector/memory T cells. The transgenic expression of HLA-DR in NOG mice did not rescue the T cell function.

Research Products

• [Journal Article] Characterization of the epithelial cell adhesion molecule (EpCAM)+ cell population in hepatocellular carcinoma cell lines. (2010)
  • Author(s): Kimura O, Takahashi T, Ishii N, Inoue Y, Ueno Y, Kogure T, Fukushima K, Shiina M, Yamagiwa Y, Kondo Y, Inoue J, Kakazu E, Iwasaki T, Kawagishi N, Shimosegawa T, Sugamura K.
  • Journal Title: Cancer Science 101 Pages: 2045-2155
  • NAID: 10027832222
  • Peer Reviewed
• [Journal Article] In vivo assay of human NK-dependent ADCC using NOD/SCID/gammac(null) (NOG) mice. (2010)
  • Author(s): Shiokawa M, Takahashi T, Murakami A, Kita S, Ito M, Sugamura K, Ishii N.
  • Journal Title: Biochem Biophys Res Commun. 399 Pages: 733-737
  • Peer Reviewed
• [Journal Article] In vivo assay of human NK-dependent ADCC using NODISCIDlgammac(null)(NOG)mice. (2010)
  • Author(s): Shiokawa M, Takahashi T, Murakami A, KitほS, Ito M, Sugamura K, Ishii N
  • Journal Title: Biochem Biophys Res Commun. Volume: 399 Pages: 733-737
  • Peer Reviewed
• [Journal Article] The analysis of the functions of human B andT cells in humanizedNOD/shi-scid/gammac(null) (NOG) mice (hu-HSC NOG mice). (2009)
  • Author(s): Watanabe Y, Takahashi T, Okajima A, Shiokawa M, Ishii N, Katano I, Ito R, Ito M, Minegishi M, Minegishi N, Tsuchiya S, Sugamura K.
  • Journal Title: Int.Immunol. 21 Pages: 843-858
  • Peer Reviewed
• [Journal Article] The analysis of human B and T cells in humanized NOG mice (hu-HSC NOG mice) (2009)
  • Author(s): Watanabe Y, Takahashi T, Okajima A, Shiokawa M, Ishii N, Katano I, Ito R, Ito M, Minegishi M, Minegishi N, Tsuchiya S, Sugamura K
  • Journal Title: International Immunology 21 Pages: 843-858
  • Peer Reviewed
• [Presentation] The analysis of the functions of human T cells in humanized NOD/shi-scid/gcnull (NOG)-HLA transgenic mice (2010)
  • Author(s): 高橋武司、鈴木真紀子、森谷邦彦、片野いくみ、伊藤亮治、伊藤守、菅村和夫、石井直人
  • Organizer: 国際免疫学会(International Congress of Immunology)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-08-27
• [Presentation] The analysis of the functions of human T cells in humanized NOD/shi-scid/γcnull (NOG)-HLA transgenic mice (2010)
  • Author(s): Takeshi Takahashi
  • Organizer: 国際免疫学会(International Congress of Immunology)
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-08-27
• [Presentation] The analysis of human B and T cells in humanized NOG mice (hu-HSC NOG mice) (2009)
  • Author(s): 高橋武司、片野いくみ、伊藤亮治、伊藤守、菅村和夫、石井直人
  • Organizer: 2^<nd> international workshop on humanized mice
  • Place of Presentation: オランダ アムステルダム
  • Year and Date: 2009-04-04
• [Presentation] Analysis of the functional human T cellsin reconstituted NOG mice (2008)
  • Author(s): 岡嶋亮良、高橋武司、渡邊庸平、片野いくみ、伊藤亮治、伊藤守、石井直人、菅村和夫
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2008-12-02
• [Presentation] Facilitating the human B cell development in humanized NOG mice (2008)
  • Author(s): 渡邊庸平、高橋武司、岡嶋亮良、片野いくみ、伊藤亮治、伊藤守、土屋滋、石井直人、菅村和夫
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2008-12-02
• [Presentation] Analysis of the functional human T cells in reconstituted NOG mice Facilitating the human B cell development in humanized NOG mice (2008)
  • Author(s): 高橋武司
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 京都国際会議場
  • Year and Date: 2008-12-02
• [Remarks] ホームページ等