Mechanism of memory CD8 T cell generation

• Project/Area Number: 20590486
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Chiba University
• Principal Investigator: SAKAMOTO Akemi Chiba University, 大学院・医学研究院, 助教 (90359597)
• Co-Investigator(Kenkyū-buntansha): TOKUHISA Takesi 千葉大学, 大学院・医学研究院, 教授 (20134364) ; HATANO Masahiko 千葉大学, 大学院・医学研究院, 教授 (20208523) ; AROMA Masafumi 千葉大学, 大学院・医学研究院, 講師 (00202763) ; FUJIMURA Riza 千葉大学, バイオメディカル研究センター, 助教 (30376363)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 免疫学 / 記憶T細胞 / 遺伝子 / 発現制御 / サイトカイン / T細胞 / 免疫記憶 / CD8T細胞 / 転写因子 / Bcl6 / KLRG1

Research Abstract

Transcription factor Bcl6 expresses in memory CD8 T cells, but its function is not elucidated. In this study, we found that KLRG1 is one of the molecular target of Bcl6. KLRG1 is known as inhibitory signaling for cell proliferation. Regulation of KLRG1 by Bcl6 might be important for keeping the function of memory CD8 T cells.

Research Products

• [Journal Article] Bcl6 is required for the development of mouse CD4+ and CD8α+ dendritic cells. (2011)
  • Author(s): Ohtsuka, H., Sakamoto, A., Pan, J., Inage, S., Horigome, S., Ichii, H., Arima, M., Hatano, M., Okada, S., Tokuhisa, T.
  • Journal Title: J.Immunol. 186 Pages: 255-263
  • Peer Reviewed
• [Journal Article] CXCR4 expression on activated B cells is down-regulated by CD63 and IL-21. (2011)
  • Author(s): Yoshida, N., Kitayama, D., Arima, M., Sakamoto, A., Inamine, A., Takano, H., Hatano, M., Koike, T., Tokuhisa, T.
  • Journal Title: J.Immunol. 186 Pages: 2800-2808
  • Peer Reviewed
• [Journal Article] Bc16 is required for the development of mouse CD4+ and CD8a+ dendritic cells. (2011)
  • Author(s): Ohtsuka, H., et al.
  • Journal Title: J.Immunol. Volume: 186 Pages: 255-263
  • Peer Reviewed
• [Journal Article] CXCR4 expression on actibated B cells is down-regulated by CD63 and IL-21. (2011)
  • Author(s): Yoshida, N., et al.
  • Journal Title: J.Immunol. Volume: 186 Pages: 2800-2808
  • Peer Reviewed
• [Journal Article] The Blimp1-Bcl6 axis is critical to regulate osteoclast differentiation and bone homeostasis. (2010)
  • Author(s): Miyauchi, Y., Ninomiya, K., Miyamoto, H., Sakamoto, A., Iwasaki, R., Hoshi, H., Miyamoto, K., Hao, W., Yoshida, S., Morioka, H., Chiba, K., Kato, S., Tokuhisa, T., Saitou, M., Toyama, Y., Suda, T., Miyamoto, T.
  • Journal Title: J Exp Med. 207 Pages: 751-762
  • Peer Reviewed
• [Journal Article] The Blimpl-Bc16 axis is critical to regulate osteoclast differentiation and bone homeostasis. (2010)
  • Author(s): Miyauchi, Y., et al.
  • Journal Title: J.Exp.Med. Volume: 207 Pages: 751-762
  • Peer Reviewed
• [Journal Article] A kelch family protein Nd1-L functions as a metastasis suppressor in cancer cells via Rho family proteins mediated mechanism. (2010)
  • Author(s): Ohta Y., et al.
  • Journal Title: Int.J.Oncology 36 Pages: 427-434
  • Peer Reviewed
• [Journal Article] A role for Bcl6 in sequential class switch recombination to IgE in B cells stimulated with IL-4 and IL-21. (2008)
  • Author(s): Kitayama, D., Sakamoto, A., Arima, M., Hatano, M., Miyazaki, M., Tokuhisa, T.
  • Journal Title: Mol.Immunol. 45 Pages: 1337-1345
  • Peer Reviewed
• [Journal Article] A Lysosomal Protein Negatively Regulates Surface T Cell Antigen Receptor Expression by Promoting CD3ζ Degradation. (2008)
  • Author(s): Ouchida, R., Yamasaki , S., Hikida, M., Masuda, K., Kawamura, K., Wada, A., Mochizuki, S., Tagawa, M., Sakamoto, A., Hatano, M., Tokuhisa, T., Koseki, H., Saito, T., Kurosaki, T., Wang, J.
  • Journal Title: Immunity 29 Pages: 33-43
  • Peer Reviewed
• [Journal Article] A role for Bcl6 in sequential class switch recombination to IgE in B cells stimulated with IL-4 and IL-21. (2008)
  • Author(s): Kitayama, D., et. al.
  • Journal Title: Mol. Immunol 45 Pages: 1337-1345
  • Peer Reviewed
• [Journal Article] A Lysosomal Protein Negatively Regulates Surface T Cell Antigen Receptor Expression by Promoting CD3 ζ Degradation (2008)
  • Author(s): Ouchida, R. et. al.
  • Journal Title: Immunity 29 Pages: 33-43
  • Peer Reviewed
• [Journal Article] A distinct role for IL -4 and IL -21 in their collaboration on proliferation and differentiation of activated B cells (2008)
  • Author(s): Saito, T. et. al.
  • Journal Title: Immunobiol 213 Pages: 545-555
  • Peer Reviewed
• [Presentation] Roles of Bcl6 in memory T cell development. (2010)
  • Author(s): Sakamoto A.
  • Organizer: 免疫記憶シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-12-04
• [Presentation] Role of Bc16 in the generation and maintenance of follicular helper T cells. (2010)
  • Author(s): Sakamoto A., et al
  • Organizer: 国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-24
• [Presentation] A role of Bc16 in differentiation of memory precursor CD8 T cells. (2010)
  • Author(s): Pan J., et al.
  • Organizer: 国際免疫学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-23
• [Presentation] A Role of Bcl6 in the generation and maintenance of follicular helper T cells. (2010)
  • Author(s): Sakamoto A, Pan J, Kohno M, Arima M, Hatano M, Tokuhisa T.
  • Organizer: 14^<th> International congress of Immunology
  • Place of Presentation: 神戸
• [Presentation] Role of Bcl6 in the generation and maintenance of follicular helper T cells. (2009)
  • Author(s): Sakamoto A, Pan J, Mochiduki A, Kohno M, Arima M, Hatano M, Tokuhisa T.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大阪
• [Presentation] Role of Bcl6 in the generation and maintenance of follicular helper T cells. (2009)
  • Author(s): Sakamoto A., et al
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大坂
• [Presentation] A role of Bcl6 in differntiation of memory precursor CD8 T cells. (2009)
  • Author(s): Pan J., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大坂
• [Presentation] A critical role of the intron enhancer element of the IL-4 gene in Th2 cytokine expression. (2009)
  • Author(s): Arima M., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大坂
• [Presentation] PHF11のマウス活性化B細胞における機能 (2009)
  • Author(s): Ikari J., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大坂
• [Presentation] IL-4 and IL-21 inversely regulate CXCR4 expression on activated B cells. (2009)
  • Author(s): Yoshida N., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大坂
• [Presentation] IgE産生細胞の維持機構の解析 (2009)
  • Author(s): Inanime A., et al.
  • Organizer: 第39回日本免疫学会
  • Place of Presentation: 大坂
• [Presentation] Altered expression of CXCR4 on actibated B cells from Bcl-6 deficient mice. (2009)
  • Author(s): Yoshida N., et al.
  • Organizer: 京都T cellカンファレンス
  • Place of Presentation: 京都
• [Presentation] Role of Bc16 in the generetion of follicular helper T cells (2008)
  • Author(s): 坂本明美
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] Role of Bcl6 in the generation of follicular helper T cells. (2008)
  • Author(s): Sakamoto A, Arima M, Hatano M, Tokuhisa T.
  • Organizer: 第38回日本免疫学会
  • Place of Presentation: 京都
• [Book] 改訂:培養細胞実験(10-4血液細胞 実験医学別冊ハンドブック)(黒木登志夫、許南浩編) (2009)
  • Author(s): 坂本明美、徳久剛史
  • Publisher: 羊土社
• [Book] 改訂:培養細胞実験ハンドブック 黒木登志夫、許南浩編、実験医学別冊 (2009)
  • Author(s): 坂本明美、徳久剛史
  • Total Pages: 329
  • Publisher: 羊土社
• [Book] 改定)培養細胞実験ハンドブック10-4血液細胞 (2008)
  • Author(s): 黒木登志夫、許南浩編、坂本明美、徳久剛史
  • Total Pages: 329
  • Publisher: 羊土社
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  • URL: http://www.m.chiba-u.ac.jp/class/devgen/
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  • URL: http://www.m.chiba-u.ac.jp/class/devgen/