Analysis of the regulatory mechanism by PRAT4A against TLR response

• Project/Area Number: 20590487
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: The University of Tokyo
• Principal Investigator: TAKAMURA Sachiko The University of Tokyo, 医科学研究所, 助教 (00325599)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: TLR / PRAT4A / LPS / エンドトキシンショック

Research Abstract

(1) The TLR4 region responsible for strong interaction with PRAT4A is very close to the site necessary for interaction with MD-2.
(2) PRAT4A differentially interacts with each TLR and suggests that a single-nucleotide change in the PRAT4A gene influences not only the strength of TLR responses but can also alter the relative activity of each TLR.
(3) Intracellular TLR4/MD-2 is responsible for unique set of LPS responses.

Research Products

• [Journal Article] A Notch ligand, Delta-like 1 functions as an adhesion molecule for mast cells. (2010)
  • Author(s): Murata A, Okuyama K, Sakano S, Kajiki M, Hirata T, Yagita H, Zuniga-Pflucker JC, Miyake K, Akashi-Takamura S, Moriwaki S, Niida S, Yoshino M, Hayashi S
  • Journal Title: J.Immunol. 185巻 Pages: 3905-3912
  • Peer Reviewed
• [Journal Article] A Notch ligand, Delta-like 1 functions as an adhesion molecule formast cells. (2010)
  • Author(s): Murata Akihiko
  • Journal Title: J.Immunol. Volume: 185 Pages: 3905-3912
  • Peer Reviewed
• [Journal Article] A single base mutation in the PRAT4A gene reveals differential interaction of PRAT4A with Toll-like receptors. (2008)
  • Author(s): Kiyokawa T, Akashi-Takamura S, Shibata T, Matsumoto F, NishitaniC, Kuroki Y, Seto Y, Miyake K
  • Journal Title: Int.Immunol. 20巻 Pages: 1407-1415
  • Peer Reviewed
• [Journal Article] TLR accessory molecules. (2008)
  • Author(s): Akashi-Takamura S, Miyake K
  • Journal Title: Curr Opin Immunol 20巻 Pages: 420-425
  • Peer Reviewed
• [Journal Article] The S100A8-serum amyloid A3-TLR4 paracrine cascade establishes a pre-metastatic phase. (2008)
  • Author(s): Hiratsuka S, Watanabe A, Sakurai Y, Akashi-Takamura S, Ishibashi S, Miyake K, Shibuya M, Akira S, Aburatani H, Maru Y
  • Journal Title: Nat Cell Biol. 10巻 Pages: 1349-55
  • Peer Reviewed
• [Journal Article] Visualization of the molecular dynamics of lipopolysaccharide on the plasma membrane of murine macrophages by total internal reflection fluorescence microscopy. (2008)
  • Author(s): Shawkat S, Karima R, Tojo T, Tadakuma H, Saitoh S, Akashi-Takamura S, Miyake K, Funatsu T, Matsushima K
  • Journal Title: J Biol Chem. 283巻 Pages: 22962-71
  • Peer Reviewed
• [Journal Article] A single base mutation in the PRAT4A gene reveals differential interaction of PRAT4A with Toll-like receptors. (2008)
  • Author(s): Kiyokawa T, Akashi-Takamura S, Shibata T, Matsumoto F, Nishitani C, Kuroki Y, Seto Y, and Miyake K
  • Journal Title: Int Immunol. 20 Pages: 1407-1415
  • Peer Reviewed
• [Journal Article] TLR accessory molecules. (2008)
  • Author(s): Akashi-Takamura S,Miyake K
  • Journal Title: Curr Opin Immunol 20 Pages: 420-425
  • Peer Reviewed
• [Journal Article] Intracellular TLR4/MD-2 in macrophages senses Gram-negative bacteria and induces a unique set of LPS-dependent genes.
  • Author(s): Shibata T., Motoi Y., TanimuraN., Yamakawa N., Akashi-Takamura S., Miyake K.
  • Journal Title: Int.Immunol. (in press)
• [Presentation] A Notch ligand, Delta-like 1 functions as an adhesion molecule. (2009)
  • Author(s): Murata A, Okuyama K, Wagatsuma K.,Moriyama T.,Sakano S, Yagita H, Miyake K, Akashi-Takamura S, Yoshino M, Hayashi S
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] A Notch ligand, Delta-like 1 functions as an adhesion molecule (2009)
  • Author(s): Murata Akihiko
  • Organizer: 第39回日本免疫学会総会・学術集会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2009-12-02
• [Presentation] A single base mutation in the PRAT4A gene reveals differential interaction of PRAT4A with Toll-like receptors. (2008)
  • Author(s): 清川貴志、高村祥子、柴田琢磨、松本文、西谷千明、黒木由夫、瀬戸泰之、三宅健介
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Detection of soluble PRAT4A (PRotein Associated with Toll-like receptor 4 ) by novel anti-PRAT4A monoclonal antibody. (2008)
  • Author(s): Shibata Takuma, Takamura Sachiko, Richard Jennings, Miyake Kensuke
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Soluble MD-1 detection using novel monoclonal antibodies. (2008)
  • Author(s): Jennings Richard, Akashi-Takamura Sachiko, Takuma Shibata, Toshihiko Kobayashi, Miyake Kensuke.
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] Detection of soluble PRAT4A by novel anti-PRAT4A monoclonal antibody. (2008)
  • Author(s): Takuma Shibata
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
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