Structural analysis of costimulatory molecules expressed on human γδ T cells
| Project/Area Number |
20590489
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Kyoto University |
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Principal Investigator |
TANAKA Yoshimasa Kyoto University, 大学院・医学研究科, 特定准教授 (90280700)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | PD-1 / PD-L1 / 抗腫瘍効果 / T細胞 / T細胞受容体 / 非ペプチド性抗原 / テトラマー / IRp60 / X線結晶解析 / BirA / ストレプトアビジン / 免疫 / カラムクロマトグラフィー / 結晶 / タンパク質 / 大腸菌 / 二次構造 / モノクローナル抗体 / 抗原 |
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| Research Abstract |
The extracellular domains of human PD-1 and PD-L1 molecules were expressed in E. coli as inclusion bodies and refolded. The refolded proteins were purified on anion-exchange column chromatography and gel filtration. IRp60, which is expressed on human γδ T cells, was similarly expressed and purified, then tetramerized. The tetramer revealed that the ligand of IRp60 was expressed on antigen-presenting cells.
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Report
(4 results)
Research Products
(50 results)
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[Journal Article] Dysregulated generation of follicular helper T cells in the spleen triggers fetal autoimmune hepatitis in mice2011
Author(s)
Nobuhiro Aoki, Masahiro Kido, Satoru Iwamoto, Hisayo Nishiura, Ryutaro Maruoka, Junya Tanaka, Takeshi Watanabe, Yoshimasa Tanaka, Taku Okazaki, Tsutomu Chiba, Norihiko Watanabe.
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Journal Title
Gastroenterology 140
Pages: 1322-1333
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[Journal Article] Rapid γδ TCR-mediated responses in γδ T cells transduced with cancer-specific TCR genes2009
Author(s)
Atsunori Hiasa, Hiroyoshi Nishikawa, Michiko Hirayama, Shigehisa Kitano, Sachiko Okamoto, Hideto Chono, Seung Shin Yu, Junichi Mineno, Yoshimasa Tanaka, Nagahiro Minato, Ikunoshin Kato, Hiroshi Shiku.
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Journal Title
Gene Therapy 16
Pages: 620-628
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[Presentation] Structure-property relationships of cellulose ethers with regioselective and blockwise substituent patterns2011
Author(s)
Hiroshi Kamitakahara, Atsushi Nakagawa, Arata Yoshinaga, Toshiyuki Takano, Tomoya Imai, Junji Sugiyama, Kaoru Hirai, Yoshimasa Tankaka, Frank Steiniger, Velina Sarbova, Dominik Fenn, Andreas Koschella, Thomas Heinze.
Organizer
241^<st> ACS National Meeting & Exposition
Place of Presentation
Anaheim Convention Center, CA, U.S.A.
Year and Date
2011-03-27
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[Presentation] Anti-PD-1 antibody reduces CD4+PD-1+ T cells and relieves the lupus-like nephritis of NZB/W2009
Author(s)
Shimpei Kasagi, Seiji Kawano, Taku Okazaki, Tasuku Honjo, Akio Morinobu, Saori, Hatachi, Kenichiro Shimatani, Yoshimasa Tanaka, Nagahiro Minato, Shunichi Kumagai
Organizer
F1 mice. American College of Rheumatology Scientific Meeting 2009
Place of Presentation
Philadelphia, PA, U.S.A.
Year and Date
2009-10-17
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[Presentation] Dual Specificity of αβ-γδ TCR T cells : Transformation of Vγ9Vδ2 T cells with MAGE-A4 143-151-specific αβ type TCR genes2008
Author(s)
Atsunori Hiasa, Hiroyoshi Nishikawa, Michiko Hirayama, Satiko Okamoto, Hideo Chono, Seung Shin Yu, Junichi Mineno, Yoshimasa Tanaka, Nagahiro Minato, Ikunoshin Kato, Hiroshi Shiku
Organizer
第14回日本遺伝子治療学
Place of Presentation
札幌医科大学、札幌市、北海道
Year and Date
2008-06-12
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