Development of a culture system to selectively induceγ δT-lymphocytes from embryonic and hematopoietic stem cells
| Project/Area Number |
20590491
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Immunology
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| Research Institution | Nagasaki International University |
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Principal Investigator |
KISHIHARA Kenji Nagasaki International University, 薬学部, 教授 (80214774)
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| Co-Investigator(Kenkyū-buntansha) |
FUJUKI Tsukasa 長崎国際大学, 薬学部, 助教 (00420612)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 自然免疫 / γδT細胞 / 幹細胞 / γδTリンパ球 / 胚性幹細胞 / 造血幹細胞 |
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| Research Abstract |
In order to develop a culture system that can selectively induce the murine gd T lymphocyte differentiation, first, the gene expression of MHC class Ib and its related molecules as potential ligands of gd T lymphocytes in several stromal cell strains were quantitatively analyzed. From the results, stromal cell strains overexpressing T10 and T22 antigens as a first choice were established. On a single layer of the stromal cells, T lymphocytes were induced from embryonic stem cells but it has not observed the fact that T10 and T22 can induce the selective and predominant differentiation of gd T lymphocytes and influence their repertoire so far. The culture system is still being further analyzed.
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Report
(4 results)
Research Products
(6 results)
