Analysis on the roles of PLCγ2 in generation and maintenance of immunological memory

• Project/Area Number: 20590497
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: Kyoto University
• Principal Investigator: HIKIDA Masaki Kyoto University, 医学研究科, 教授 (60228715)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 免疫学 / 遺伝子 / 動物 / 病理学 / ゲノム / 胚中心 / PLC γ 2 / 記憶応答 / PLCγ2 / 記憶B細胞 / 抗体産生

Research Abstract

In spite of previous researches, molecular basis for the development and survival of memory B cells remains largely unknown. In this study, we tried to clarify the roles of PLCγ2 on such molecular machinery. As the result, we found that PLCγ2 is playing a pivotal role in the formation of germinal center, where memory B cells are formed. It was also revealed that PLCγ2 is essential for the maintenance of the memory B cells.

Research Products

• [Journal Article] Ca^<2+>influx and protein scaffolding via TRPC3 sustain PKCβand ERK activationin B cells. (2010)
  • Author(s): 疋田正喜, 他10名
  • Journal Title: J.Cell.Sci. Volume: 123 Pages: 927-938
  • Peer Reviewed
• [Journal Article] PLC-γ2 is essential for formation and maintenance of memory B cells. (2009)
  • Author(s): Hikida M, Casola S, Takahashi N, Kaji T, Takemori T, Rajewsky K, Kurosaki T.
  • Journal Title: J.Exp.Med. 206(3) Pages: 681-9
  • Peer Reviewed
• [Journal Article] Tyrosine kinases and their substrates in Blymphocytes. (2009)
  • Author(s): Kurosaki T, Hikida M.
  • Journal Title: Immunol.Rev. 228(1) Pages: 132-48
  • Peer Reviewed
• [Journal Article] PLCγ2 is essential for formation and maitenance of memory B cells (2009)
  • Author(s): 疋田正喜
  • Journal Title: J. Exp. Med. vol 206 Pages: 681-689
  • Peer Reviewed
• [Journal Article] Tyrosine kinases and their substrates in B lymphocytes. (2009)
  • Author(s): 疋田正喜
  • Journal Title: immunol. Rev. vol 228 Pages: 132-148
  • Peer Reviewed
• [Journal Article] PLCγ2 is essential for formation and maintenance of memory B cells. (2009)
  • Author(s): Hikida M., Casola S., Takahashi N., Kaji T., Takemori T., Rajewsky K., Kurosaki T.
  • Journal Title: J. Exp. Med. 206 Pages: 681-689
  • Peer Reviewed
• [Journal Article] Essential roles of mgcRacGAP in multilineage differentiation and survival of murine hematopoietic cells. (2008)
  • Author(s): Yamada T., Kurosaki T., Hikida M.
  • Journal Title: Biochem. Biophys. Res. Commun. 372 Pages: 941-946
  • Peer Reviewed
• [Presentation] IgG1 memory B cell compartment undergoes qualitative alteration after its initial generation early in the immune response (2010)
  • Author(s): 疋田正喜, 他4名
  • Organizer: 14^<th> Internadonal congress of immunology
  • Place of Presentation: 神戸市、神戸国際展示場(招待講演)
  • Year and Date: 2010-08-26
• [Presentation] PLCγ2 is essential for formation and maintenance of memory B cells. (2008)
  • Author(s): Masaki Hikida
  • Organizer: 日本免疫学会、シンポジウム
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-12-01
• [Presentation] PLCγ2 is essential for formation and maintenance of memory B cells (2008)
  • Author(s): Hikida M., Casola S., Takahashi N., Kaji T., Takemori T., Rajewsky K., Kurosaki T.
  • Organizer: 日本免疫学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2008-12-01