Functional analysis of memory B cells in lungs

• Project/Area Number: 20590501
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Immunology
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TAKAHASHI Yoshimasa National Institute of Infectious Diseases, 免疫部, 室長 (60311403)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 免疫記憶 / インフルエンザ / B細胞

Research Abstract

Lung memory B cells exhibited the increased maturity and conferred protection against re-infected viruses by producing robust numbers of IgA-secreting plasma cells. Lung memory B cells showed the similar gene expression profile with those in secondary lymphoid organs, but several genes were selectively upregulated in lung memory B cells. Gene-deficient mice were generated to define in vivo function of one of those genes.

Research Products

• [Journal Article] Lack of antibody response to Guillain-Barre syndrome-related gangliosides in mice and men after novel influenza vaccination. (2011)
  • Author(s): Yuki, et al.
  • Journal Title: J.Neurol.Neurosurg.Psychiatry Volume: (掲載確定)
  • Peer Reviewed
• [Journal Article] Lack of antibody response to Guillain-Barre syndrome-related gangliosides in mice and men after novel influenza vaccination. (2010)
  • Author(s): Yuki N., Takahashi Y., Ihara T., Ito S., Nakajima T., Funakoshi K., Furukawa K., Kobayashi K., Odaka M.
  • Journal Title: J.Neurol.Neurosurg.Psychiatry.
  • Peer Reviewed
• [Journal Article] Unique properties of memory B cells of different isotypes. (2010)
  • Author(s): Kurosaki T., Aiba Y., Kometani K., Moriyama S., Takahashi Y.
  • Journal Title: Immunol.Rev. 237 Pages: 104-116
• [Journal Article] CD22 expression mediates the regulatory functions of peritoneal B-la cells during the remission phase of contact hypersensitivity reactions. (2010)
  • Author(s): Nakashima H., Hamaguchi Y., Watanabe R., Ishiura N., Kuwano Y., Okochi H., Takahashi Y., Tamaki K., Sato S., Tedder T. F., Fujimoto M.
  • Journal Title: J.Immuno. 184 Pages: 637-4645
  • Peer Reviewed
• [Journal Article] Differential phosphorylation of functional tyrosines in CD19 modulates B lymphocyte activation. (2010)
  • Author(s): Ishiura N. Nakashima H., Watanabe R., Kuwano Y., Adachi T., Takahashi Y., Tsubata T., Okochi H., Tamaki K., Tedder T. F., Fujimoto M.
  • Journal Title: Eur.J.Immunol. 40 Pages: 1192-1204
  • Peer Reviewed
• [Journal Article] Regulation of antigen-receptor gene assembly in hagfish. (2010)
  • Author(s): Kishishita N., Matsuno T., Takahashi Y., Takaba H., Nishizumi H., Nagawa F.
  • Journal Title: EMBO.Rep. 11 Pages: 126-132
  • Peer Reviewed
• [Journal Article] Unique properties of memory B cells of different isotypes. (2010)
  • Author(s): Kurosaki, et al.
  • Journal Title: Immunol.Rev. Volume: 237 Pages: 104-116
  • Peer Reviewed
• [Journal Article] CD22 expression mediates the regulatory functions of peritoneal B-la cells during the remission phase of contact hypersensitivity reactions. (2010)
  • Author(s): Nakashima, et al.
  • Journal Title: J.Immunol. Volume: 184 Pages: 4637-4645
  • Peer Reviewed
• [Journal Article] Differential phosphorylation of functional tyrosines in CD19 modulates B lymphocyte activation (2010)
  • Author(s): Ishiura, et al.
  • Journal Title: Eur.J.Immunol. 40 Pages: 1192-1204
  • Peer Reviewed
• [Journal Article] Protective immunity afforded by H5N1 (NIBRG-14)-inactivated vaccine requires both antibodies against hemagglutinin and neuraminidase. (2009)
  • Author(s): Takahashi Y., Hasegawa H., Hara Y., Ato M., Ninomiya A., Takagi H., Odagiri T., Sata T., Tashiro M., Kobayashi K.
  • Journal Title: J.Infect.Dis. 199 Pages: 1629-1637
  • Peer Reviewed
• [Journal Article] Bacteroides induce higher IgA production than Lactobacillus by increasing activation-induced cytidine deaminase expression on B cells in murine peyer's patches. (2009)
  • Author(s): Yanagibashi T., Hosono A., Oyama A., Tsuda M., Hachimura S., Takahashi Y., Itoh K., Hirayama K., Takahashi K., Kaminogawa S.
  • Journal Title: Biosci.Biotech.Biochem. 73 Pages: 372-377
  • NAID: 10027537465
  • Peer Reviewed
• [Journal Article] Protective immunity afforded by H5N1(NIBRG-14)-inactivated vaccine requires both antibodies against hemagglutinin and neuraminidase (2009)
  • Author(s): Takahashi, et al.
  • Journal Title: J.Infect.Dis. 199 Pages: 1629-1637
  • Peer Reviewed
• [Journal Article] Bacteroides induce higher IgA production than Lactobacillus by increasing activation-induced cytidine deaminase expression on B cells in murine peyer's patches (2009)
  • Author(s): Yanagibashi, et al.
  • Journal Title: Biosci. Biotech. Biochem 73 Pages: 372-377
  • Peer Reviewed
• [Presentation] Protective memory B cells against influenza virus infection in the lungs. (2010)
  • Author(s): 高橋宜聖
  • Organizer: 千葉大G-COEシンポジウム(Development and maintenance of immunememory)
  • Place of Presentation: 東京
  • Year and Date: 2010-12-04
• [Presentation] Protective memory B cells against influenza virus infection in the lungs (2010)
  • Author(s): 高橋宜聖
  • Organizer: 千葉大学G-COEシンポジウム
  • Place of Presentation: 東京都千代田区東京ステーションコンファレンス
  • Year and Date: 2010-12-04
• [Presentation] IgGI memory B cell compartment undergoes qualitative alteration after its initial generation early in the immune response. (2010)
  • Author(s): Hikida M
  • Organizer: 14th International Congress of Immunology.
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-26
• [Presentation] IgG1 memory B cell compartment undergoes qualitative alternation after its initial generation early in the immune response. (2010)
  • Author(s): Hikida, et al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 兵庫県神戸市神戸ポートピアホテル、神戸国際展示場
  • Year and Date: 2010-08-26
• [Presentation] T-cell independent activation of virus-specific memory B cells requires Toll-like receptor(TLR)signaling. (2010)
  • Author(s): Onodera, et al.
  • Organizer: 14^<th> International Congress of Immunology
  • Place of Presentation: 兵庫県神戸市神戸ポートピアホテル、神戸国際展示場
  • Year and Date: 2010-08-26
• [Presentation] T-cell independent activation of virus-specific memory B cells requires Toll-like receptor (TLR) signaling. (2010)
  • Author(s): Onodera T
  • Organizer: 4th International conference on" B cells and autoimmunity"
  • Place of Presentation: 奈良
  • Year and Date: 2010-08-20
• [Presentation] T-cell independent activation of virus-specific memory B cells requires Toll-like receptor(TLR)signaling. (2010)
  • Author(s): Onodera, et al.
  • Organizer: 14^<th> International conference on B cells and autoi mmunity
  • Place of Presentation: 奈良県奈良市奈良ロイヤルホテル
  • Year and Date: 2010-08-20
• [Presentation] T-cell independent activation of virus-specific memory B cells requires Toll-like receptor (TLR) signaling. (2010)
  • Author(s): Onodera T
  • Organizer: 14th International Congress of Immunology.
  • Place of Presentation: 神戸
  • Year and Date: 2010-08-06
• [Presentation] Bacteroidesの刺激を受けたバイエル板細胞におけるIgA誘導の特徴 (2010)
  • Author(s): 大山尭人
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] IL-5を高産生する腸管NK様細胞の同定 (2010)
  • Author(s): 小川晋平
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-29
• [Presentation] Non-mutated memory B cells develop under T-cell help without germinal center reaction, followed by the functional maturation as immune response progresses. (2009)
  • Author(s): 加地友弘
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] Tce11-independent activation of memory B cells with B-2 phenotype by whole virus particles. (2009)
  • Author(s): 小野寺大志
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] Non-mutated memory B cells develop under T-cell help without germinal center reaction, followed by the functional maturation as immune response progresses (2009)
  • Author(s): 加地友弘
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] T cell-independent activation of memory B cells with B-2 phenotype by whole virus particles (2009)
  • Author(s): 小野寺大志
  • Organizer: 日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-03
• [Presentation] 腸管特有の細胞群CD3-IL-2R+細胞の表現型および機能解析 (2009)
  • Author(s): 小川晋平
  • Organizer: 第39回日本免疫学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2009-12-02
• [Presentation] ヒト血清移入マウスを用いたインフルエンザウイルス感染防御能の解析 (2009)
  • Author(s): 高橋宜聖
  • Organizer: 第13回日本ワクチン学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-27
• [Presentation] ヒト血清移入マウスを用いたインフルエンザウイルス感染防御能の解析 (2009)
  • Author(s): 高橋宜聖
  • Organizer: 日本ワクチン学会学術集会
  • Place of Presentation: 札幌
  • Year and Date: 2009-09-27
• [Presentation] ヒト血清抗体・細胞移入マウスを用いた防御免疫評価系の構築 (2009)
  • Author(s): 高植宜聖
  • Organizer: 日本食品免疫学会2009年度大会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-26
• [Presentation] マウス腸内共生菌が誘導する腸管IgA産生の特徴 (2009)
  • Author(s): 大山尭人
  • Organizer: 日本食品免疫学会2009年度大会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-26
• [Presentation] 腸管特有の細胞群CD3-IL-2R+細胞の機能解析 (2009)
  • Author(s): 小川晋平
  • Organizer: 日本食品免疫学会2009年度大会
  • Place of Presentation: 東京
  • Year and Date: 2009-05-26
• [Presentation] ヒト血清抗体・細胞移入マウスを用いた防御免疫評価系の構築 (2009)
  • Author(s): 高橋宜聖
  • Organizer: 日本食品免疫学会
  • Place of Presentation: 東京大学
  • Year and Date: 2009-05-26
• [Presentation] マウス腸内共生菌が誘導する腸管IgA産生応答についてのin vitroでの解析 (2009)
  • Author(s): 大山尭人
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] 腸管特有の細胞群CD3-IL-2R+細胞の機能解析 (2009)
  • Author(s): 小川晋平
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] BacteroidesはB細胞のAID発現を誘導し腸管IgA産生を充進する (2009)
  • Author(s): 柳橋努
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-29
• [Presentation] インフルエンザウイルスの感染防御に寄与するメモリーB細胞の同定 (2009)
  • Author(s): 高橋宜聖
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] バクテロイデスは胚中心の形成を促すことで大腸のIgA産生を強く誘導する (2008)
  • Author(s): 高橋宜聖
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] バイエル板CD3-IL-2R+細胞によるクラススイッチの誘導およびIgA分泌の促進 (2008)
  • Author(s): Ogawa S
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] バクテロイデスは胚中心の形成を促すことで大腸のIgA産生を強く誘導する (2008)
  • Author(s): 柳橋努
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] パイエル板CD3-IL-R+細胞によるクラススイッチの誘導およびIgA分泌の促進 (2008)
  • Author(s): 小川晋平
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-03
• [Presentation] B細胞と免疫器官におけるBILL-cadherin/cadherin-17の発現パターン (2008)
  • Author(s): Fu S
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] IgA+ memory B cells persist in the lung after an intranasal infection with influenza virus. (2008)
  • Author(s): 高橋宜聖
  • Organizer: 第38回日本免疫学会総会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] B細胞と免疫器官におけるBILL-cadherin/cadherin-17の発現パターン (2008)
  • Author(s): 傅船一
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] IgA+ memory B cells persist in the lung after an intranasal infection with influenza virus (2008)
  • Author(s): 高橋宜聖
  • Organizer: 日本免疫学会
  • Place of Presentation: 京都
  • Year and Date: 2008-12-02
• [Presentation] H5N1(NIBRG-14)ワクチンの感染防御効果には、抗ヘマグルチニン抗体と抗ノイラミニダーゼ抗体の両者が関与する (2008)
  • Author(s): 高橋宜聖
  • Organizer: 第12回日本ワクチン学会学術集会
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-08
• [Presentation] ワクチンの感染防御効果には、抗ヘマグルチニン抗体と抗ノイラミニダーゼ抗体の両者が関与する (2008)
  • Author(s): 高橋宜聖
  • Organizer: 日本ワクチン学会
  • Place of Presentation: 熊本
  • Year and Date: 2008-11-08
• [Remarks] ホームページ等