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Development of the analysis method of proteins in breast cancer for predictdrug sensitivity the prediction of drug sensitivity

Research Project

Project/Area Number 20590532
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied pharmacology
Research InstitutionHirosaki University

Principal Investigator

ODAGIRI Hiroki (2009-2010)  Hirosaki University, 大学院・医学研究科, 准教授 (60250601)

立石 智則 (2008)  Hirosaki University, 大学院・医学研究科, 教授 (80236554)

Co-Investigator(Kenkyū-buntansha) TATEISHI Tomonori  弘前大学, 大学院・医学研究科, 教授 (80236554)
小田桐 弘毅  弘前大学, 医学部・附属病院, 講師 (60250601)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords癌 / 蛋白質 / プロテオーム / 薬物反応性 / 薬剤感受性 / 乳がん / 発現蛋白 / 薬物感受性
Research Abstract

In the first two years, optimal condition of the two dimensional gel electrophoresis using proteins from breast cancer tissue was decided.
Optimal condition was as follows: applied protein amount for gel was 30μg, pH range was 3-10, voltage was 500v 1h, 1000v 2h, then 8000v 2.5h in the first dimension polyacrylamide gel electrophoresis. Clear dots pattern was obtained in 10mA/gel 1.5h for the second demensional gel electrophoresis. 2D-DIGE (2 dimensional-differential gel electrophoresis) was selected to compare the amount of proteins between the cancer tissue and the non-cancerous tissue quantitatively. Protein from cancer tissue was labeled by Cy3 and protein from non-cancerous tissue was lebeled by Cy5. Difference of the amount of proteins from two kind of tissue was shown by the difference of the intensity of those two kinds of fluorescence.
In 2010, tissue from removed from ten breast cancer patients was tested.
Several dots, which showed difference in the intensity of fluorescence between cancer and non-cancerous tissue, were observed. Those should be candidates of protein that are important when cancer shows malignant characters.
In near future, we will identify the proteins from those dots which differ between cancer and non-cancerous tissue for the better understanding of the carcinogenesis.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (9 results)

All 2011 2010 2008 Other

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] The suicide gene introduction using two recombinant adenovirus vectors with HER2 promoter and Cre/loxP system.2011

    • Author(s)
      Masateru Maruyama, Hiroki-Odagiri, et al
    • Journal Title

      Hirosaki Med J Vol 61, No2-4

      Pages: 150-158

    • NAID

      110008425054

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] The suicide gene introduction using two recombinant adenovuus vector with HER2 promoter and Cre/loxP system2011

    • Author(s)
      丸山将輝、小田桐弘毅
    • Journal Title

      The Hirosaki Medical Journal

      Volume: 61(2-4) Pages: 150-158

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient gene transduction in HER2-expressing cancer cells by two recombinant virus vectors with Her2 promotor and Cre/loxP system.2010

    • Author(s)
      Tatsuya Nodagashira, Hiroki Odagiri, et al
    • Journal Title

      Hirosaki Med J Vol 61, No1

      Pages: 26-34

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Efficient gene transduction in HER2-expressing cancer cells by two recombinant adenovirus vectors with HER2 promoter and Cre/loxP system2010

    • Author(s)
      野田頭達也, 小田桐弘毅
    • Journal Title

      The Hirosaki Medical Journal 60(1)

      Pages: 25-34

    • NAID

      110007569124

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The different effects of itraconazole on the pharmacokinetics of fe xofenadine enantiomers2008

    • Author(s)
      Tateishi T
    • Journal Title

      Br J Clin Pharmacol 65(5)

      Pages: 693-700

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Chemosensitivity determinants of irinotecan hydrochloride in hepat ocellular carcinoma cell lines2008

    • Author(s)
      Takahata T
    • Journal Title

      Basic Clin Pharmacol Toxicol 102(4)

      Pages: 399-407

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The role of cytochrome P2C19 in R-warfarin pharmacokinetics and its interaction with omeorazole2008

    • Author(s)
      Uno T
    • Journal Title

      Ther Drug Monit 30(3)

      Pages: 276-281

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] P糖蛋白を介した薬物相互作用の臨床研究-CYP3Aとの比較-2008

    • Author(s)
      立石智則
    • Organizer
      第23回日本薬物動態学会年会
    • Place of Presentation
      熊本市
    • Year and Date
      2008-11-01
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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