Proteomic analysis of thrombopoietin signaling

• Project/Area Number: 20590544
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied pharmacology
• Research Institution: Osaka City University
• Principal Investigator: NAKAO Takafumi Osaka City University, 大学院・医学研究科, 講師 (50326261)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: ファーマコゲノミクス / プロテオーム / 細胞内シグナル伝達 / 細胞内シグナル伝逹

Research Abstract

Thrombopietin is the most important cytokine for thrombopoiesis. The importance of thrombopoietin signaling is immediately apparent; morbidity and mortality from bleeding due to moderate to severe thrombocytopenia is a major problem of a wide range of patients. The aim of this study is to find the molecules that interact with Akt, serine-threonine kinase which plays a crucial role for megakaryocytopoiesis, in human hematopoietic cells. To this end, we established the affinity purification and proteomic analysis to elucidate the role of Akt signaling in megakaryocytopoiesis. However, we could not detect Akt-binding protein under thrombopoietin-dependent manner.

Research Products

• [Journal Article] Severe prekallikrein deficiency due to a homozygous Trp499Stop nonsense mutation. (2011)
  • Author(s): Nakao T, Yamane T, Katagami T, Shiota M, Izumi Y, Samori T, Hino M, Iwao H.
  • Journal Title: Blood Coagul Fibrinolysis. 22 Pages: 337-339
  • Peer Reviewed
• [Journal Article] 喫煙者を対象とした電子タバコの安全性確認試験 (2011)
  • Author(s): 三浦克之、菊川友子、中尾隆文、東海秀吉、泉康雄、藤井比佐子、北條泰輔
  • Journal Title: 生活衛生 Volume: 55 Pages: 59-64
  • NAID: 40018770368
  • Peer Reviewed
• [Journal Article] Heat shock cognate protein 70 is essential for Akt signaling in endothelial function. (2010)
  • Author(s): Shiota M, Kusakabe H, Izumi Y, Hikita Y, Nakao T, Funae Y, Miura K, Iwao H.
  • Journal Title: Arterioscler Tromb Vasc Biol. 30 Pages: 491-497
  • NAID: 10026525817
  • Peer Reviewed
• [Journal Article] Heat shock cognate protein 70 is essential for Akt signaling in endothelial function. (2010)
  • Author(s): Shiota M, Kusakabe H, Izumi Y, Hikita Y, Nakao T, Funae Y, Miura K, Iwao H.
  • Journal Title: Arterioscler Thromb Vasc Biol. Volume: 30 Pages: 491-497
  • NAID: 10026525817
  • Peer Reviewed
• [Journal Article] 二重盲検単回摂取クロスオーバー試験によるss-グルカン強化型大麦食品の食後血糖値への影響および安全性の検討 (2010)
  • Author(s): 中尾隆文、藤井比佐子、菊川友子、東海秀吉、久下高生、椿和文、松本渉、三宅真澄、金田俊光、橋本文彦、三浦克之、平山佳伸
  • Journal Title: 薬理と治療 Volume: 38 Pages: 904-914
  • Peer Reviewed
• [Journal Article] 同種末梢血幹細胞移植後の小腸穿孔で診断されたEpstein-Barr virus関連移植後リンパ増殖性疾患 (2010)
  • Author(s): 相本瑞樹、山根孝久、井上敦司、百瀬大、相本蘭、井上恵里、岡本秀一郎、康秀男、中根孝彦、武岡康信、中前美佳、小坂さおり、寺田芳樹、中前博久、高起良、中尾隆文、大澤政彦、日野雅之
  • Journal Title: 臨床血液 Volume: 51 Pages: 1775-1780
  • Peer Reviewed
• [Journal Article] Heat shock cognate protein 70 is essential for Akt signaling in endothelial function. (2010)
  • Author(s): Shiota M, Kusakabe H, Izumi Y, Hikita Y, Nakao T, Funae Y, Miura K, Iwao H.
  • Journal Title: Arterioscler Thromb Vasc Biol. 30 Pages: 491-497
  • NAID: 10026525817
  • Peer Reviewed
• [Journal Article] Effects of liposome clodronate on renal leukocyte populations and renal fibrosis in murine obstructive nephropathy. (2009)
  • Author(s): Kitamoto K, Machida Y, Uchida J, Izumi Y, Shiota M, Nakao T, Iwao H, Yukimura T, Nakatani T, Miura K.
  • Journal Title: J Pharmacol Sci. 111 Pages: 285-292
  • NAID: 10026154246
  • Peer Reviewed
• [Journal Article] Pravastatin accelerates ischemia-induced angiogenesis through AMP-activated protein kinase. (2009)
  • Author(s): Izumi Y, Shiota M, Kusakabe H, Hikita Y, Nakao T, Nakamura Y, Muro T, Miura K, Yoshiyama M, Iwao H.
  • Journal Title: Hypertens Res. 32 Pages: 675-679
  • NAID: 10025164814
  • Peer Reviewed
• [Journal Article] Effects of metoprolol on epinephrine-induced takotsubo-like left ventricular dysfunction in non-human primates. (2009)
  • Author(s): Izumi Y, Okatani H, Shiota M, Nakao T, Ise R, Kito G, Miura K, Iwao H.
  • Journal Title: Hypertens Res. 32 Pages: 339-346
  • NAID: 10026122410
  • Peer Reviewed
• [Journal Article] Pharmacogenomics of cardiovascular pharmacology : Molecular network analysis in pleiotropic effects of statin-an experimental elucidation of the pharmacologic action from protein-protein interaction analysis. (2008)
  • Author(s): Shiota M, Kusakabe H, Hikita Y, Nakao T, Izumi Y, Iwao H.
  • Journal Title: J Pharmacol Sci. 107 Pages: 15-19
  • NAID: 10024320291
  • Peer Reviewed
• [Journal Article] Pharmacogenomics of Cardiovascular Pharmacology : Molecular Network Analysis in Pleiotropic Effects of Statin-an Experimental Elucidation of the Pharmacologic Action From Protein-Protein Interaction Analysis (2008)
  • Author(s): Shiota, M., Kusakabe, H., Hikita, Y., Nakao, T., Izumi, Y., Iwao, H.
  • Journal Title: J Pharmacol Sci 107 Pages: 15-19
  • NAID: 10024320291
  • Peer Reviewed
• [Presentation] Hsp70sインタラクトーム解析による障害タンパク質の高効率同定 (2011)
  • Author(s): 塩田正之、文沙耶、田中昌子、中尾隆文、泉康雄、三浦克之、岩尾洋
  • Organizer: 第84回薬理学会年会
  • Place of Presentation: 横浜(誌上開催)
  • Year and Date: 2011-03-24