The role of micro RNA and methylated gene in multistep genesis and prognosis of multiple myeloma

Research Project

Project/Area Number	20590556
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Laboratory medicine
Research Institution	Gunma University
Principal Investigator	HANDA Hiroshi Gunma University, 医学部, 講師 (90282409)
Co-Investigator(Kenkyū-buntansha)	MURAKAMI Hirokazu 群馬大学, 医学部, 教授 (40166260) OGAWARA Hatsue 群馬大学, 医学部, 教授 (60134293)
Project Period (FY)	2008 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	骨髄腫 / 多段階発癌 / メチル化遺伝子 / microRNA / micro RNA / 予後
Research Abstract	We detected methylated WWOX promoter in myeloma cell lines, myeloma patients but not in MGUS patients. The aberrant transcript of WWOX mRNA was detected in myeloma patients, MGUS and cell lines. The expression level of micro-RNA miR15a 15b, 16 were very low or lacked in purified plasma cells in some patients. MiR 15a, 15b, 16 levels were lower in plasma cells of MM than those of MGUS. On the contrary, Dnmts and Mcl-1 levels were higher in plasma cells of MM than those of MGUS.