Development of the hypersensitive and rapid detection method for NF-κB activation using by Fluorescence Correlation Spectroscopy and its application to the emergency medicine

• Project/Area Number: 20590559
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: University of Toyama
• Principal Investigator: KITAJIMA Isao University of Toyama, 大学院・医学薬学研究部(医学), 教授 (50214797)
• Co-Investigator(Kenkyū-buntansha): NIIMI Hideki 富山大学, 附属病院検査部, 副部長,助教 (50401865) ; HAKAKEYAMA Noboru 富山大学, 附属病院手術部, 副部長,准教授 (70251907)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 転写因子 / NF-κB / 1分子蛍光相関法 / 全身炎症反応症候群 / 救急医療 / 1分子蛍光法 / 炎症性サイトカイン / 迅速検査 / 全身性炎症反応候群 / 菌血症 / 全身性炎症反応症候群 / 一分子蛍光法 / 核蛋白抽出法

Research Abstract

We have established a novel measurement system for the NF-κB activity using fluorescence correlation spectroscopy (FCS), which was a methodology to examine the size and number of fluorescence-labeled molecules in a confocal area by direct observation of their Brownian motion in solutions. The principle of the measurement was to detect the different of molecular weights of fluorescent-labeled DNA probe and DNA probe-NF-κB complex. The elevation of NF-κB activity reflects the acute inflammation. Measuring the NF-κB activity in the patients with systemic inflammatory response syndrome (SIRS) would be useful as an inflammatory marker.

Research Products

• [Journal Article] Aberrant CaMKII activity is associated with abnormal dendoricspine morphology in ATRX mutant mouse brain. (2011)
  • Author(s): Shioda N, Beppu H, Fukuda T, Li E, Kitajima I, Fukunaga K
  • Journal Title: J Neurosci Volume: 31(1) Pages: 346-358
  • Peer Reviewed
• [Journal Article] Venous thromboembolism after spinal surgery : Changes of the fibrin monomer complex and D-dimer level during the perioperative period. (2010)
  • Author(s): Yoshioka K, Kitajima I, Kabata T, Tani M, Kawahara N, Murakami H, Demura S, Tsubokawa T, Tomita K
  • Journal Title: Journal of Neurosurgery. Spine 13(5) Pages: 594-599
  • Peer Reviewed
• [Journal Article] Venous thromboembolism after spinal surgery : Changes of the fibrin monomer complex and D-dimer level during the perioperative period. (2010)
  • Author(s): Yoshioka K, Kitajima I, Kabata T, Tani M, Kawahara N, Murakami H, Demura S, Tsubokawa T, Tomita K
  • Journal Title: Journal of Neurosurgery. Spine Volume: 13(5) Pages: 594-599
  • Peer Reviewed
• [Journal Article] Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice. (2010)
  • Author(s): Nogami T, Beppu H, Tokoro T, Moriguchi S, Shioda N, Fukunaga K, Ohtsuka T, Ishii Y, Sasahara M, Shimada Y, Nishijo H, Li E, Kitajima I
  • Journal Title: HIPPOCAMPUS Volume: 21巻
  • Peer Reviewed
• [Journal Article] Induction of autophagic cell death and radiosensitization by the pharmacological inhibition of nuclear factor-kappa B ctication in human glioma cell lines. (2009)
  • Author(s): Tsuboi Y, Kurimoto M, Nagai S, Hayakawa Y, Kamiyama H, Hayashi N, Kitajima I, Endo S
  • Journal Title: J.Neurosurg. 110(3) Pages: 594-604
  • Peer Reviewed
• [Journal Article] Important regulatory role of activated platelet-derived procoagulant activity in the propagation of thrombi formed under arterial blood flow conditions. (2009)
  • Author(s): Tamura N, Kitajima I, Kawamura Y, Toda E, Eguchi Y, Ishida H, Goto S
  • Journal Title: Circ J. 73(3) Pages: 540-548
  • NAID: 110007126721
  • Peer Reviewed
• [Journal Article] Diagnosis of cerebral cortical vein thrombosis with T2^*-weighted magnetic resonance imaging. (2009)
  • Author(s): Katayama Y, Kitajima I, Niimi H, Kitahara H, Kawai H
  • Journal Title: Brit Med J Case Reports doi:10.1136/bcr.03.2009.1651
  • Peer Reviewed
• [Journal Article] Pharmacological significance of the blocking action of the intravenous general anesthetic propofol on the slow component of cardiac delayed rectifier K+ current. (2009)
  • Author(s): Hatakeyama N, Sakuraya F, Matsuda N, Kimura J, Kinoshita H, Kemmotsu O, Yamazaki M, Hattori Y
  • Journal Title: J.Pharmacol Sci. 110 Pages: 334-343
  • NAID: 10025737753
  • Peer Reviewed
• [Journal Article] severity of obstructive speep apnea syndrome may affect postoperative hypoxemia in morbidly obese patients. (2009)
  • Author(s): Kinoshita H, Hatakeyama N, Minonishi T, Hatano Y
  • Journal Title: Aneth Analg 108 Pages: 1044-1044
  • Peer Reviewed
• [Journal Article] Induction of autophagic cell death and radiosensitization by the pharmacological inhibition of nuclear factor-kappa B activation in human glioma cell lines. (2009)
  • Author(s): Tsuoi Y, Kurimoto M, Nagai S, Kamiyama H, Hayashi N, Kitajima I, Endo S
  • Journal Title: J.Neurosurg. 110(3) Pages: 594-604
  • Peer Reviewed
• [Journal Article] Important regulatory role of activated platelet-derived procoagulant activity in the propagation of thrombi formed under arterial blood flow conditions. (2009)
  • Author(s): Tamura N, Kitajima I, Kawamura Y, Toda E., Eguchi Y, Ishida H, Goto S
  • Journal Title: Circ J 73(3) Pages: 323-334
  • NAID: 110007126721
  • Peer Reviewed
• [Journal Article] Pharmacological significance of the blocking action of the intravenous general anesthetic propofol on the slow component of cardiac delayed rectifier K+ current (2009)
  • Author(s): Hakakeyama N, Sakuraya F, Matsuda N, Kimura J, Kinoshita H, Kemmotsu O, Yamazaki M, Hattiri Y
  • Journal Title: J.Pharmacol Sci 110(3) Pages: 334-343
  • NAID: 10025737753
  • Peer Reviewed
• [Journal Article] Effects of different statins on endothelial nitric oxide synthase and AKT phosphorylation in endothelial cells. (2008)
  • Author(s): Wang J, Xu Z, Kitajima I, Wang Z
  • Journal Title: International Journal of Cardiology 127(1) Pages: 33-39
  • Peer Reviewed
• [Journal Article] Cholinesterase inhibitor donepezil dilates cerebral parenchymal arterioles via the activation of neuronal nitric oxide synthase. (2008)
  • Author(s): Nakahata K, Kinoshita H, Ishida Y, Matsuda N, Hatakeyama N, Kondo T, Hatano Y
  • Journal Title: Anethesiology 109 Pages: 124-129
  • Peer Reviewed
• [Journal Article] Modulation of glucocorticoid receptor expression, inflammation and cell apoptosis in septic guinea-pig lung using methylprednisolone. (2008)
  • Author(s): Kamiyama K, Matsuda N, Yamamoto S, Takano K, Takano Y, Yamazaki H, Kageyama SI, Kokoo H, Nagata H, Hatakeyama N, Tsukada K, Hattori Y
  • Journal Title: Am J Physiol Lung Cell Mol Physiol. 259
  • Peer Reviewed
• [Journal Article] Role of phosphatidylinositol 3-kinase and NADHP oxidase in ATP-sensitive K+ channel function impaired by high glucose in the human artery. (2008)
  • Author(s): Kinoshita H, Matsuda N, Hatakeyama N, Azma T, Kuroda Y, Tange K, Hatano Y
  • Journal Title: Hypertension 52 Pages: 507-513
• [Journal Article] Effects of different statins on endothelial nitric oxide synthase and AKT phosphorylation in endothelial cells. (2008)
  • Author(s): Wang J, Xu Z, Kitajima I, Wang Z
  • Journal Title: International Journal of Cardiology 127 Pages: 33-39
  • Peer Reviewed
• [Journal Article] Changes of the soluble fibrin monomer complex level in the perioperative period of hip replacement surgery. (2008)
  • Author(s): Misaki, T., Kitajima, I., Kabata, T., Tani, et. al.
  • Journal Title: J Orthop Sci 13 Pages: 419-424
  • Peer Reviewed
• [Journal Article] Modulation of glucocirticoid receptor expression, inflammation, and cell apoptosis in septic guinea pig lungs using methylprednisolone. (2008)
  • Author(s): Kamiya K, Matsuda N, Yamamoto S, Takano K, Yamazaki H, Hakakeyama N
  • Journal Title: Am I Physiology Lung Cell Mol Physiol 295
  • Peer Reviewed
• [Presentation] Nuclear factor-kappa B activity correlates with metabolic syndrome-related biomerkers in patients with essential hypertension. (2010)
  • Author(s): Harada K, Kitajima I
  • Organizer: 2010 AACC (American Association for Clinical Chemistry)
  • Place of Presentation: Anaheim, CA, USA
  • Year and Date: 2010-07-27
• [Presentation] Anti-inflammatory effect of pitavastatin on NF-kaoppa B activated by TNF-alfa through Rho kinase pathway in breast cancer cells. (2010)
  • Author(s): Kitajima I
  • Organizer: Keystone Symposia 2010 NF-kappa B in inflammation and Disease
  • Place of Presentation: Santa Fe, USA
  • Year and Date: 2010-01-08
• [Presentation] Anti-inflammatory effect of pitavastatin on NF-kaoppa B activated TNF-alfa through Rho kinase pathway in breast cancer cells. (2010)
  • Author(s): Kitajima I
  • Organizer: Keystone Symposia 2010 NF-kappa B in inflammation and Disease
  • Place of Presentation: Santa Fe, USA
• [Presentation] Nuclear factor-kappa B activity correlates with metabolic syndrome-related biomerkers in patients with essential hypertension. (2010)
  • Author(s): Harada K, Kitajima I
  • Organizer: 2010 AACC (American Association for Clinical Chemistry) Annaul Meetin
  • Place of Presentation: Anaheim, CA, USA
• [Presentation] Fastest ID system for detecting septic bacteris. 2009 Bio International Convention. (2009)
  • Author(s): Niimi H, Kitajima I
  • Organizer: The Global event for Biotechnology.
  • Place of Presentation: Atlanta, USA
• [Presentation] Role of Nuclear factor-kappa B (NF-κB) in tumor progresion. (2008)
  • Author(s): Kitajima I
  • Organizer: The 1^<st> International traditional chinese medicine (TCM) and Interative Medicine Oncology Formum
  • Place of Presentation: Shanghai, China.
  • Year and Date: 2008-10-18
• [Presentation] Role of Nuclear factor-kappa B (NF-κB)in tumor progresion. (2008)
  • Author(s): Kitajima I
  • Organizer: The 1^<st> International traditional chinese medicine (TCM) and Interative Medicine Oncology Formum.
  • Place of Presentation: Shanghai, China.
  • Year and Date: 2008-10-18
• [Presentation] A new assay for NF-κB activity in biological samples using fluorescence correlation spectroscopy and its application to perioperative cardiac monitoring. (2008)
  • Author(s): Kitajima I, Note R, Ogawa T, Abe S, Kadowaki M, Yamagata K
  • Organizer: 2008 Keystone symposia NF-κB.
  • Place of Presentation: Banff, Canada
• [Book] 臨床検査の原点は診察室にあり。医療を変える机の上の検査室 (2009)
  • Author(s): 北島勲
  • Total Pages: 248
  • Publisher: 緒方医学化学研究所(東京)
• [Book] 凝固・線溶・血小板機能検査。血液・造血器疾患学(小澤敬也、直江知樹、坂田洋一編) (2008)
  • Author(s): 北島 勲
  • Total Pages: 301
  • Publisher: Medical view社(東京)
• [Remarks] ホームページ等