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Development of the hypersensitive and rapid detection method for NF-κB activation using by Fluorescence Correlation Spectroscopy and its application to the emergency medicine

Research Project

Project/Area Number 20590559
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionUniversity of Toyama

Principal Investigator

KITAJIMA Isao  University of Toyama, 大学院・医学薬学研究部(医学), 教授 (50214797)

Co-Investigator(Kenkyū-buntansha) NIIMI Hideki  富山大学, 附属病院検査部, 副部長,助教 (50401865)
HAKAKEYAMA Noboru  富山大学, 附属病院手術部, 副部長,准教授 (70251907)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords転写因子 / NF-κB / 1分子蛍光相関法 / 全身炎症反応症候群 / 救急医療 / 1分子蛍光法 / 炎症性サイトカイン / 迅速検査 / 全身性炎症反応候群 / 菌血症 / 全身性炎症反応症候群 / 一分子蛍光法 / 核蛋白抽出法
Research Abstract

We have established a novel measurement system for the NF-κB activity using fluorescence correlation spectroscopy (FCS), which was a methodology to examine the size and number of fluorescence-labeled molecules in a confocal area by direct observation of their Brownian motion in solutions. The principle of the measurement was to detect the different of molecular weights of fluorescent-labeled DNA probe and DNA probe-NF-κB complex. The elevation of NF-κB activity reflects the acute inflammation. Measuring the NF-κB activity in the patients with systemic inflammatory response syndrome (SIRS) would be useful as an inflammatory marker.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (30 results)

All 2011 2010 2009 2008 Other

All Journal Article (19 results) (of which Peer Reviewed: 18 results) Presentation (8 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Aberrant CaMKII activity is associated with abnormal dendoricspine morphology in ATRX mutant mouse brain.2011

    • Author(s)
      Shioda N, Beppu H, Fukuda T, Li E, Kitajima I, Fukunaga K
    • Journal Title

      J Neurosci

      Volume: 31(1) Pages: 346-358

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Venous thromboembolism after spinal surgery : Changes of the fibrin monomer complex and D-dimer level during the perioperative period.2010

    • Author(s)
      Yoshioka K, Kitajima I, Kabata T, Tani M, Kawahara N, Murakami H, Demura S, Tsubokawa T, Tomita K
    • Journal Title

      Journal of Neurosurgery. Spine 13(5)

      Pages: 594-599

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Venous thromboembolism after spinal surgery : Changes of the fibrin monomer complex and D-dimer level during the perioperative period.2010

    • Author(s)
      Yoshioka K, Kitajima I, Kabata T, Tani M, Kawahara N, Murakami H, Demura S, Tsubokawa T, Tomita K
    • Journal Title

      Journal of Neurosurgery. Spine

      Volume: 13(5) Pages: 594-599

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice.2010

    • Author(s)
      Nogami T, Beppu H, Tokoro T, Moriguchi S, Shioda N, Fukunaga K, Ohtsuka T, Ishii Y, Sasahara M, Shimada Y, Nishijo H, Li E, Kitajima I
    • Journal Title

      HIPPOCAMPUS

      Volume: 21巻

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Induction of autophagic cell death and radiosensitization by the pharmacological inhibition of nuclear factor-kappa B ctication in human glioma cell lines.2009

    • Author(s)
      Tsuboi Y, Kurimoto M, Nagai S, Hayakawa Y, Kamiyama H, Hayashi N, Kitajima I, Endo S
    • Journal Title

      J.Neurosurg. 110(3)

      Pages: 594-604

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Important regulatory role of activated platelet-derived procoagulant activity in the propagation of thrombi formed under arterial blood flow conditions.2009

    • Author(s)
      Tamura N, Kitajima I, Kawamura Y, Toda E, Eguchi Y, Ishida H, Goto S
    • Journal Title

      Circ J. 73(3)

      Pages: 540-548

    • NAID

      110007126721

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Diagnosis of cerebral cortical vein thrombosis with T2^*-weighted magnetic resonance imaging.2009

    • Author(s)
      Katayama Y, Kitajima I, Niimi H, Kitahara H, Kawai H
    • Journal Title

      Brit Med J Case Reports doi:10.1136/bcr.03.2009.1651

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacological significance of the blocking action of the intravenous general anesthetic propofol on the slow component of cardiac delayed rectifier K+ current.2009

    • Author(s)
      Hatakeyama N, Sakuraya F, Matsuda N, Kimura J, Kinoshita H, Kemmotsu O, Yamazaki M, Hattori Y
    • Journal Title

      J.Pharmacol Sci. 110

      Pages: 334-343

    • NAID

      10025737753

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] severity of obstructive speep apnea syndrome may affect postoperative hypoxemia in morbidly obese patients.2009

    • Author(s)
      Kinoshita H, Hatakeyama N, Minonishi T, Hatano Y
    • Journal Title

      Aneth Analg 108

      Pages: 1044-1044

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of autophagic cell death and radiosensitization by the pharmacological inhibition of nuclear factor-kappa B activation in human glioma cell lines.2009

    • Author(s)
      Tsuoi Y, Kurimoto M, Nagai S, Kamiyama H, Hayashi N, Kitajima I, Endo S
    • Journal Title

      J.Neurosurg. 110(3)

      Pages: 594-604

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Important regulatory role of activated platelet-derived procoagulant activity in the propagation of thrombi formed under arterial blood flow conditions.2009

    • Author(s)
      Tamura N, Kitajima I, Kawamura Y, Toda E., Eguchi Y, Ishida H, Goto S
    • Journal Title

      Circ J 73(3)

      Pages: 323-334

    • NAID

      110007126721

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacological significance of the blocking action of the intravenous general anesthetic propofol on the slow component of cardiac delayed rectifier K+ current2009

    • Author(s)
      Hakakeyama N, Sakuraya F, Matsuda N, Kimura J, Kinoshita H, Kemmotsu O, Yamazaki M, Hattiri Y
    • Journal Title

      J.Pharmacol Sci 110(3)

      Pages: 334-343

    • NAID

      10025737753

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of different statins on endothelial nitric oxide synthase and AKT phosphorylation in endothelial cells.2008

    • Author(s)
      Wang J, Xu Z, Kitajima I, Wang Z
    • Journal Title

      International Journal of Cardiology 127(1)

      Pages: 33-39

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Cholinesterase inhibitor donepezil dilates cerebral parenchymal arterioles via the activation of neuronal nitric oxide synthase.2008

    • Author(s)
      Nakahata K, Kinoshita H, Ishida Y, Matsuda N, Hatakeyama N, Kondo T, Hatano Y
    • Journal Title

      Anethesiology 109

      Pages: 124-129

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of glucocorticoid receptor expression, inflammation and cell apoptosis in septic guinea-pig lung using methylprednisolone.2008

    • Author(s)
      Kamiyama K, Matsuda N, Yamamoto S, Takano K, Takano Y, Yamazaki H, Kageyama SI, Kokoo H, Nagata H, Hatakeyama N, Tsukada K, Hattori Y
    • Journal Title

      Am J Physiol Lung Cell Mol Physiol. 259

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Role of phosphatidylinositol 3-kinase and NADHP oxidase in ATP-sensitive K+ channel function impaired by high glucose in the human artery.2008

    • Author(s)
      Kinoshita H, Matsuda N, Hatakeyama N, Azma T, Kuroda Y, Tange K, Hatano Y
    • Journal Title

      Hypertension 52

      Pages: 507-513

    • Related Report
      2010 Final Research Report
  • [Journal Article] Effects of different statins on endothelial nitric oxide synthase and AKT phosphorylation in endothelial cells.2008

    • Author(s)
      Wang J, Xu Z, Kitajima I, Wang Z
    • Journal Title

      International Journal of Cardiology 127

      Pages: 33-39

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Changes of the soluble fibrin monomer complex level in the perioperative period of hip replacement surgery.2008

    • Author(s)
      Misaki, T., Kitajima, I., Kabata, T., Tani, et. al.
    • Journal Title

      J Orthop Sci 13

      Pages: 419-424

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Modulation of glucocirticoid receptor expression, inflammation, and cell apoptosis in septic guinea pig lungs using methylprednisolone.2008

    • Author(s)
      Kamiya K, Matsuda N, Yamamoto S, Takano K, Yamazaki H, Hakakeyama N
    • Journal Title

      Am I Physiology Lung Cell Mol Physiol 295

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Nuclear factor-kappa B activity correlates with metabolic syndrome-related biomerkers in patients with essential hypertension.2010

    • Author(s)
      Harada K, Kitajima I
    • Organizer
      2010 AACC (American Association for Clinical Chemistry)
    • Place of Presentation
      Anaheim, CA, USA
    • Year and Date
      2010-07-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Anti-inflammatory effect of pitavastatin on NF-kaoppa B activated by TNF-alfa through Rho kinase pathway in breast cancer cells.2010

    • Author(s)
      Kitajima I
    • Organizer
      Keystone Symposia 2010 NF-kappa B in inflammation and Disease
    • Place of Presentation
      Santa Fe, USA
    • Year and Date
      2010-01-08
    • Related Report
      2009 Annual Research Report
  • [Presentation] Anti-inflammatory effect of pitavastatin on NF-kaoppa B activated TNF-alfa through Rho kinase pathway in breast cancer cells.2010

    • Author(s)
      Kitajima I
    • Organizer
      Keystone Symposia 2010 NF-kappa B in inflammation and Disease
    • Place of Presentation
      Santa Fe, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Nuclear factor-kappa B activity correlates with metabolic syndrome-related biomerkers in patients with essential hypertension.2010

    • Author(s)
      Harada K, Kitajima I
    • Organizer
      2010 AACC (American Association for Clinical Chemistry) Annaul Meetin
    • Place of Presentation
      Anaheim, CA, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Fastest ID system for detecting septic bacteris. 2009 Bio International Convention.2009

    • Author(s)
      Niimi H, Kitajima I
    • Organizer
      The Global event for Biotechnology.
    • Place of Presentation
      Atlanta, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] Role of Nuclear factor-kappa B (NF-κB) in tumor progresion.2008

    • Author(s)
      Kitajima I
    • Organizer
      The 1^<st> International traditional chinese medicine (TCM) and Interative Medicine Oncology Formum
    • Place of Presentation
      Shanghai, China.
    • Year and Date
      2008-10-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Role of Nuclear factor-kappa B (NF-κB)in tumor progresion.2008

    • Author(s)
      Kitajima I
    • Organizer
      The 1^<st> International traditional chinese medicine (TCM) and Interative Medicine Oncology Formum.
    • Place of Presentation
      Shanghai, China.
    • Year and Date
      2008-10-18
    • Related Report
      2008 Annual Research Report
  • [Presentation] A new assay for NF-κB activity in biological samples using fluorescence correlation spectroscopy and its application to perioperative cardiac monitoring.2008

    • Author(s)
      Kitajima I, Note R, Ogawa T, Abe S, Kadowaki M, Yamagata K
    • Organizer
      2008 Keystone symposia NF-κB.
    • Place of Presentation
      Banff, Canada
    • Related Report
      2010 Final Research Report
  • [Book] 臨床検査の原点は診察室にあり。医療を変える机の上の検査室2009

    • Author(s)
      北島勲
    • Total Pages
      248
    • Publisher
      緒方医学化学研究所(東京)
    • Related Report
      2009 Annual Research Report
  • [Book] 凝固・線溶・血小板機能検査。血液・造血器疾患学(小澤敬也、直江知樹、坂田洋一編)2008

    • Author(s)
      北島 勲
    • Total Pages
      301
    • Publisher
      Medical view社(東京)
    • Related Report
      2008 Annual Research Report
  • [Remarks] ホームページ等

    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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