| Project/Area Number |
20590563
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Shinshu University |
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Principal Investigator |
HIDAKA Hiroya Shinshu University, 医学部, 准教授 (10362138)
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| Co-Investigator(Kenkyū-buntansha) |
OTA Iroyoshi 信州大学, 医学部, 教授 (50273107)
HONDA Takayuki 信州大学, 医学部, 教授 (80238815)
AKAMATSU Taiji 信州大学, 医学部附属病院, 准教授 (80212413)
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| Research Collaborator |
TAKIWAKI Masaki 信州大学, 大学院・医学系研究科, 博士前期課程
MITANI Atsushi 信州大学, 大学院・医学系研究科, 博士前期課程
YAMASHITA Mine 信州大学, 大学院・医学系研究科, 博士前期課程
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 臨床化学 / 脂質 / リン脂質 / 質量分析 / 含硫アミノ酸 / コリン含有リン脂質 / 酵素法 |
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| Research Abstract |
For investigation of a rapid measurement system and its application to a laboratory study of the biochemical markers reflecting the clinical condition of inflammatory bowel disease, we analyzed the concentration, composition, and structure of molecular abnormalities of lipid and protein metabolism in human serum. We established methods for analysis of phospholipids and their metabolites, as well as modified protein with sulfur-containing amino acids, cysteine, and homocysteine, using matrix-associated ionization time-of-flight mass spectrometry (MALDI-TOF-MS), gas chromatography electron impact mass spectrometry (GC/MS), and high-performance liquid chromatography (HPLC). The inflammation index of the patient was high, but levels of lipids, apoproteins, and sulfur-containing amino acids were low, and the phospholipids showed a characteristic composition.
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