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Analyze the mechanism of onset of Graves' disease, using high sensitivity method for TSH receptor antibody

Research Project

Project/Area Number 20590569
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory medicine
Research InstitutionOsaka University

Principal Investigator

HIDAKA Yoh  Osaka University, 医学系研究科, 准教授 (30243231)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsTSHレセプター抗体 / バセドウ病 / スギ花粉症 / アレルギー性鼻炎
Research Abstract

The peripheral blood mononuclear cells (PBMC) from the Graves' patients complicated with cedar pollen allergy were stimulated with cedar pollen. TSH receptor antibody (TRAb) could be detected in the supernatant using high sensitivity method. However, TRAb also could be detected in the supernatant incubated without the stimulation of cedar pollen. Moreover, TRAb could be detected in the supernatant incubated PBMC from the Graves' patients without cedar pollen allergy. Therefore, we could not demonstrate that the cedar pollen stimulates the production of TRAb.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (37 results)

All 2011 2010 2009 2008 Other

All Journal Article (23 results) (of which Peer Reviewed: 23 results) Presentation (12 results) Book (2 results)

  • [Journal Article] Association of functional polymorphisms in promoter regions of IL5,IL6 and IL13 genes with development and prognosis of autoimmune thyroid diseases.2011

    • Author(s)
      Inoue N, Watanabe M, Morita M, Tatsumi K, Hidaka Y, Akamizu T, Iwatani Y.
    • Journal Title

      Clin Exp Immunol 163(3)

      Pages: 318-323

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Association of functional polymorphisms in promoter regions of IL5, IL6 and IL13 genes with development and prognosis of autoimmune thyroid diseases2011

    • Author(s)
      Inoue N, Watanabe M, Morita M, Tatsumi K, Hidaka Y, Akamizu T, Iwatani Y
    • Journal Title

      Clin Exp Immunol

      Volume: 163 Pages: 318-323

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of functional polymorphisms related to the transcriptional level of FOXP3 with prognosis of autoimmune thyroid diseases.2010

    • Author(s)
      Inoue N, Watanabe M, Morita M, Tomizawa R, Akamizu T, Tatsumi K, Hidaka Y, Iwatani Y.
    • Journal Title

      Clin Exp Immunol 162(3)

      Pages: 402-406

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Messenger RNA quantification after fluorescence activated cell sorting using intracellular antigens.2010

    • Author(s)
      Yamada H, Maruo R, Watanabe M, Hidaka Y, Iwatani Y, Takano T.
    • Journal Title

      Biochem Biophy Res Commun 397(3)

      Pages: 425-428

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Low level of factor V is associated with development of deep-vein thrombosis in Japanese patients.2010

    • Author(s)
      Suehisa E, Kawasaki T, Toku M, Hidaka Y.
    • Journal Title

      Thromb Res 125(2)

      Pages: 128-133

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Postpartum increase of serum thioredoxin concentrations and the relation to CD8 lymphocytes.2010

    • Author(s)
      Kuroda S, Watanabe M, Santo T, Shimizuishi Y, Takano T, Hidaka Y, Kimura T, Iwatani Y.
    • Journal Title

      Ann Clin Biochem 47(Pt1)

      Pages: 62-66

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Messenger RNA quantification after fluorescence activatedd cell sorting using intracellular antigens2010

    • Author(s)
      Yamada H, Maruo R, Watanabe M, Hidaka Y, Iwatani Y, Takano T
    • Journal Title

      Biochem Biophy Res Commun

      Volume: 397 Pages: 425-428

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of functional polymorphisms related to the transcriptional level of FOXP3 with prognosis of autoimmune thyroid diseases2010

    • Author(s)
      Inoue N, Watanabe M, Morita M, Tomizawa R, Akamizu T, Tatsumi K, Hidaka Y, Iwatani Y
    • Journal Title

      Clin Exp Immunol

      Volume: 162 Pages: 402-406

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Postpartum increase of serum thioredoxin concentrations and the relation to CD8 lymphocytes2010

    • Author(s)
      Kuroda S, Watanabe M, Santo T, Shimizuishi Y, Takano T, Hidaka Y, Kimura T, Iwatani Y
    • Journal Title

      Ann Clin Biochem 47

      Pages: 62-66

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low level of factor V is associated with development of deep-vein thrombosis in Japanese patients2010

    • Author(s)
      Suehisa E, Kawasaki T, Toku M, Hidaka Y
    • Journal Title

      Thromb Res 125

      Pages: 128-133

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Outpatient administration of radioactive iodine after total thyroidectomy for pediatric thyroid cancer : A report of three cases.2009

    • Author(s)
      Takano T, Yane K, Oue T, Otsuki N, Nibu K, Hidaka Y.
    • Journal Title

      Int J Pediatr Otorhi 73(12)

      Pages: 1810-1813

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Early detection of metastasis and relapse of colorectal cancer by recognizing NCA-2 in commercially available CEA assay.2009

    • Author(s)
      Hanada H, Mugii S, Takeoka K, Maeda I, Watanabe M, Hidaka Y, Iwatani Y.
    • Journal Title

      Clin Chem 55(9)

      Pages: 1747-1748

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Acquired activated protein C resistance is associated with IgG antibodies to protein S in patients with systemic lupus erythematosus.2009

    • Author(s)
      Nojima J, Iwatani Y, Ichihara K, Ishikawa T, Tsuneoka H, Yanagihara M, Takano T, Hidaka Y.
    • Journal Title

      Thromb Res 124(1)

      Pages: 127-131

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Increased expression levels of tensin3 mRNA in thyroid functional adenomas as compared to non-functioning adenomas.2009

    • Author(s)
      Maeda I, Yamada H, Takano T, Nishihara E, Ito Y, Matsuzuka F, Miya A, Kobayashi K, Yoshida H, Miyauchi A, Hidaka Y.
    • Journal Title

      Exp Clin Endocr Diab 117

      Pages: 191-193

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Early detection of metastasis and relapse of colorectal cancer by recognizing NCA-2 in commercially available CEA assay2009

    • Author(s)
      Hanada H, Mugii S, Takeoka K, Maeda I, Watanabe M, Hidaka Y, Iwatani Y
    • Journal Title

      Clin Chem 55

      Pages: 1747-1748

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Acquired activated protein C resistance is associated with IgG antibodies to protein S in patients with systemic lupus erythematosus.2009

    • Author(s)
      NojimaJ, IwataniY, IchiharaK, IshikawaT, TsuneokaH, YanagiharaM, TakanoT, HidakaY
    • Journal Title

      Thromb Res (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequencies of mild factor V, VII and X deficiencies in a Japanese population.2008

    • Author(s)
      Yasunaga C, Suehisa E, Toku M, Kawasaki T, Hidaka Y.
    • Journal Title

      Blood Coagul Fibrinolysis 19(6)

      Pages: 597-600

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous transient Graves' thyrotoxicosis.2008

    • Author(s)
      Hidaka Y, Tatsumi K.
    • Journal Title

      Thyroid 18(10)

      Pages: 1133-1134

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spontaneous transient Graves' thyrotoxicosis.2008

    • Author(s)
      Hidaka Y, Tatsumi K
    • Journal Title

      Thyroid 18

      Pages: 1133-1134

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Frequencies of mild factor V, VII and X deficiencies in a Japanese population.2008

    • Author(s)
      Yasunaga c, Suehisa E, Toku M, Kawasaki T, Hidaka Y
    • Journal Title

      Blood Coagul Fibrinolysis 19

      Pages: 597-600

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association of functional GITR gene polymorphisms related to expression of GITR molecules with prognosis of autoimmune thyroid disease.

    • Author(s)
      Tomizawa R, Watanabe M, Inoue N, Takemura K, Hidaka Y, Akamizu T, Hayakawa K, Iwatani Y.
    • Journal Title

      Clin Exp Immunol in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a new modified Bethesda method for coagulation inhibitors : the Osaka modified Bethesda method.

    • Author(s)
      Torita S, Suehisa E, Kawasaki T, Toku M, Takeo E, Tomiyama Y, Nishida S, Hidaka Y.
    • Journal Title

      Blood Coagul Fibrinolysis in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] mRNA quantification after fluorescence activated cell sorting using locked nucleic acid probes.

    • Author(s)
      Maruo R, Yamada H, Watanabe M, Hidaka Y, Iwatani Y, Takano T.
    • Journal Title

      Mol Biotechnol in press

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] TFF3/LGALS3 mRNA発現比定量による甲状腺癌の穿刺吸引核酸診断法の臨床検討2010

    • Author(s)
      山田宏哉、高野徹、木原実、廣川満良、吉田博、渡邉幹夫、岩谷良則、日高洋、宮内昭
    • Organizer
      日本甲状腺学会
    • Place of Presentation
      長崎
    • Year and Date
      2010-11-12
    • Related Report
      2010 Final Research Report
  • [Presentation] GITR遺伝子多型と自己免疫性甲状腺疾患の難治度・重症度との関連2010

    • Author(s)
      武村和哉、冨澤理恵、2渡邉幹夫、井上直哉、早川和生、赤水尚史、日高洋、岩谷良則
    • Organizer
      日本臨床化学会年会
    • Place of Presentation
      山梨
    • Year and Date
      2010-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] 新規臨床検査法、FACS-mQの開発2010

    • Author(s)
      高野徹、日高洋、岩谷良則
    • Organizer
      日本臨床検査意学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-11
    • Related Report
      2010 Final Research Report
  • [Presentation] 凝固因子インヒビター測定の新たなべセスダ法変法の開発2010

    • Author(s)
      鳥田澄子、末久悦次、徳雅幸、竹尾映美、川崎富夫、日高洋
    • Organizer
      日本検査血液学会
    • Place of Presentation
      東京
    • Year and Date
      2010-07-24
    • Related Report
      2010 Final Research Report
  • [Presentation] Analysis of pituitary-rich KIAA1324/maba1 in prolactin secretion.2010

    • Author(s)
      Kousaka K, Tatsumi K, Hidaka Y
    • Organizer
      14th International Congress of Endocrinology
    • Place of Presentation
      京都
    • Year and Date
      2010-03-30
    • Related Report
      2010 Final Research Report
  • [Presentation] Preoperative diagnosis of thyroid nodules by measuresing TFF3/LGALS3 mRNA (T/G) ratio : a clinical trial of fifty cases.2010

    • Author(s)
      Yamada H, Takano T, Kihara M, Hirokawa Y, Yoshida H, Watanabe M, Iwatani Y, Hidaka Y, Miyauchi A
    • Organizer
      14th International Congress of Endocrinology
    • Place of Presentation
      京都
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] An in-tube in situ hybridization method for the novel stem cell detection system, FACS-mQ.2009

    • Author(s)
      Maruo R, Yamada H, Takano T, Watanabe M, Iwatani Y, Hidaka Y
    • Organizer
      The 9th Asia and Oceania Thyroid Association Congress
    • Place of Presentation
      名古屋
    • Year and Date
      2009-11-02
    • Related Report
      2010 Final Research Report
  • [Presentation] An in-tube in situ hybridization method for the novel stem cell detection system, FACS-mQ2009

    • Author(s)
      Maruo R, Yamada H, Takano T, Watanabe M, Iwatani Y, Hidaka Y
    • Organizer
      The 9th Asia and Oceania Thyroid Association Congress
    • Place of Presentation
      名古屋
    • Year and Date
      2009-11-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] 簡便な患者検体結果の代表値(中央値)の評価と管理検体結果と組み合わせた後方視的な利用法の検討2009

    • Author(s)
      巽圭太、須崎由起子、松井昌彦、竹岡啓子、林貞夫、渡邉幹夫、岩谷良則、日高洋
    • Organizer
      日本臨床検査意学会総会
    • Place of Presentation
      札幌
    • Year and Date
      2009-08-27
    • Related Report
      2010 Final Research Report
  • [Presentation] 第29回イムノアッセイ検査全国コントロールサーベイ成績報告2009

    • Author(s)
      家入蒼生夫、池田斉、小田桐恵美、片上秀喜、亀子光明、桑克彦、竹岡啓子、武田京子、日高洋
    • Organizer
      日本内分泌学会学術総会
    • Place of Presentation
      前橋
    • Year and Date
      2009-04-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 一過性バセドウ病の3例2008

    • Author(s)
      日高洋、巽圭太、高野徹
    • Organizer
      日本甲状腺学会
    • Place of Presentation
      宇都宮
    • Year and Date
      2008-11-22
    • Related Report
      2010 Final Research Report 2008 Annual Research Report
  • [Presentation] LISのデータチェックロジック2008

    • Author(s)
      松井昌彦、正司浩規、木村茂樹、末久悦次、前田育宏、井山茂、林貞夫、日高洋
    • Organizer
      日本臨床化学会年会
    • Place of Presentation
      浜松
    • Year and Date
      2008-08-29
    • Related Report
      2010 Final Research Report
  • [Book] バセドウ病(p.174-180)バセドウ病眼症(p.181-184)無痛性甲状腺炎(p.188-191)亜急性甲状腺炎(p.192-194)甲状腺クリーぜ(p.195-198)妊娠と甲状腺疾患(p.211-215)

    • Author(s)
      日高洋
    • Publisher
      医薬ジャーナル内分泌代謝疾患・糖尿病診療マニュアル(改訂版)2009
    • Related Report
      2010 Final Research Report
  • [Book] よくわかる甲状腺疾患のすべて(改訂第2版)2009橋本病の診断(p.31-35)/日高洋、網野信行:橋本病と妊娠、産後の異常(p.254-258)

    • Author(s)
      網野信行、高野徹、日高洋
    • Publisher
      永井書店
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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