Development of tumor diagnosis method using cancer/testis antigens.

• Project/Area Number: 20590571
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Okayama University
• Principal Investigator: ONO Toshiro 岡山大学, 自然生命科学研究支援センター, 准教授 (50185641)
• Co-Investigator(Kenkyū-buntansha): HANAFUSA Tadashi 岡山大学, 自然生命科学研究支援センター, 助教 (00228511)
• Co-Investigator(Renkei-kenkyūsha): UENAKA Akiko 岡山大学,川崎医療福祉大学, 医学部, 客員研究員,研究員 (50273967)
• Project Period (FY): 2008 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: がん・精巣抗原 / SEREX / 抗体 / 抗原 / ELISA / 腫瘍マーカー / リアルタイム / RT-PCR / 遺伝子 / DNAマイクロアレイ / リアルタイムRT-PCR / がん抗原 / CT抗原 / 血清 / 診断 / ウエスタンブロット / がん

Research Abstract

Cancer/testis(CT) antigens are expressed in normal germ cell line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. We performed serological identification of antigens by recombinant expression cloning to identify CT antigens. The humoral immune response of cancer patients against the newly defined CT antigens was confirmed. This study indicated that the newly defined CT antigens facilitates their use as diagnostic reagents and establishes a testing and diagnostic method effective to cancers.

Research Products

• [Journal Article] 新規がん抗原GKAP1の同定と口腔扁平上皮癌での発現解析 (2011)
  • Author(s): 銅前昇平、小野俊朗、佐々木朗
  • Journal Title: 日本口腔外科学雑誌 Volume: 57 Pages: 542-547
  • NAID: 10030272891
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies (2011)
  • Author(s): T. Hanafusa, A. M. Ali Eldib, K. Kitaoka, Y. Ohue, E. Nakayama and T. Ono
  • Journal Title: Cancer Letters Volume: 301 Pages: 57-62
  • NAID: 120003437013
  • Peer Reviewed
• [Journal Article] 新規がん抗原GKAP1の同定と口腔扁平上皮癌での発現解析 (2011)
  • Author(s): 銅前昇平
  • Journal Title: 日本口腔外科学会会誌 Volume: 57 Pages: 542-547
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies. (2011)
  • Author(s): T. Hanafusa, A.M. Ali Eldib, K. Kitaoka, Y. Ohue, E. Nakayama, T. Ono
  • Journal Title: Cancer Letters 301 Pages: 57-62
  • NAID: 120003437013
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies. (2011)
  • Author(s): Hanafusa, T.
  • Journal Title: Cancer Letters Volume: 301 Pages: 57-62
  • NAID: 120003437013
  • Peer Reviewed
• [Journal Article] Targeting KRAS mutation-bearing lung cancer in vivo by pulmonary surfactant-adenovirus-mediated gene transfer (2010)
  • Author(s): T. Fukazawa, Y. Maeda, J. Matsuoka, T. Ono(12名、4番目)
  • Journal Title: Anticancer Research Volume: 30 Pages: 4925-4935
  • Peer Reviewed
• [Journal Article] Targeting KRAS mutation-bearing lung cancer in vivo by pulmonary surfactant-adenovirus-mediated gene transfer. (2010)
  • Author(s): T. Fukazawa, Y. Maeda, J. Matsuoka, T. Ono(12名、4番目)
  • Journal Title: Anticancer Research 30 Pages: 4925-4935
  • Peer Reviewed
• [Journal Article] Targeting KRAS mutation-bearing lung cancer in vivo by pulumonary surfactant-adenovirus-mediated gene transfer. (2010)
  • Author(s): Fukazawa, T.
  • Journal Title: Anti Cancer Research Volume: 30 Pages: 4925-4935
  • Peer Reviewed
• [Journal Article] Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity (2009)
  • Author(s): S. Domae, Y. Nakamura, (17名、17番目)
  • Journal Title: International Journalof Cancer Volume: 124 Pages: 2347-2352
  • Peer Reviewed
• [Journal Article] Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity. (2009)
  • Author(s): S. Domae, Y. Nakamura, Y. Nakamura(17名、17番目)
  • Journal Title: International Journal of Cancer 124 Pages: 2347-2352
  • Peer Reviewed
• [Journal Article] Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity. (2009)
  • Author(s): Domae, S.
  • Journal Title: Int. J. Cancer 124 Pages: 2347-2352
  • Peer Reviewed
• [Journal Article] Identification of CCDC62-2 as a novel cancer/testis antigen and its immunogenicity. (2009)
  • Author(s): S. Domae, et al.
  • Journal Title: International Journal of Cancer 124 Pages: 2347-2352
  • Peer Reviewed
• [Journal Article] Identification of a CD4 T-cell epitope in the rejection antigen from BALB/c radiation-induced leukemia RL♂1 (2008)
  • Author(s): S. Kaya, A. Uenaka, S. Sto, T. Ono, T. T. Aji and E. Nakayama
  • Journal Title: Cancer Science Volume: 99 Pages: 1141-1147
  • Peer Reviewed
• [Journal Article] Identification of a CD4 T-cell epitope in the rejection antigen from BALB/c radiation-induced leukemia RL♂1. (2008)
  • Author(s): S. Kaya, A. Uenaka, S. Sto, T. Ono, T. Aji , E. Nakayama
  • Journal Title: Cancer Science 99 Pages: 1141-1147
  • Peer Reviewed
• [Journal Article] Identification of a CD4 T-cell epitope in the rejection antigen from BALB/c radiation induced leukemia RL♂1. (2008)
  • Author(s): S. Kaya, et al.
  • Journal Title: Cancer Science 99 Pages: 1141-1147
  • Peer Reviewed
• [Presentation] Humoral immune response of cancer patients against newly SEREX-defined antigen (2011)
  • Author(s): S. Domae, T. Hanafusa, M. Otsuka, M. Muraoka, A. Sasaki, E. Nakayama and T. Ono
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-05
• [Presentation] Construction of phage scFv antibody against Protocadherin 11, a new tumor antigen candidate of prostate cancer (2011)
  • Author(s): T. Hanafusa, S. Domae and T. Ono
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2011-10-05
• [Presentation] Humoral immune response of cancer patients against newly SEREX-defined antigen, GKAP1 (2011)
  • Author(s): S.Domae
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Consruction of phage scFv antibody against Protocadherin 11, a new tumor antigen candidate of prostate cancer (2011)
  • Author(s): T.Hanafusa
  • Organizer: 第70回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies (2010)
  • Author(s): T. Hanafusa, K. Kitaoka, Y. Ohue, E. Nakayama and T. Ono
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] Targeting KRAS lung cancer in vivo by pulmonary surfactant-mediatedgene transfer (2010)
  • Author(s): T. Fukazawa, J. Matsuoka, T. Ono(9名、3番目)
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies. (2010)
  • Author(s): T.Hanafusa, K.Kitaoka, Y.Ohue, E.Nakayama, T.Ono
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] Targeting KRAS lung cancer in vivo by pulmonary surfactant-mediated gene transfer. (2010)
  • Author(s): T. Fukazawa, J. Matsuoka, T.Ono(9名、3番目)
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] Targeting KRAS lung cancer in vivo by pulumonary surfactant-mediated gene transfer. (2010)
  • Author(s): Fukazawa, T.
  • Organizer: 第69回日本癌学会学術総会
  • Place of Presentation: 大阪市
  • Year and Date: 2010-09-24
• [Presentation] SEREX法による肺腺癌抗原の同定と発現解析 (2010)
  • Author(s): 花房直志、北岡賢太、大植祥弘、中山睿一、小野俊朗
  • Organizer: 第14回日本がん免疫学会総会
  • Place of Presentation: 熊本市
  • Year and Date: 2010-07-22
• [Presentation] Simultaneous expression of multiple splice variants of PCDH11X/Y in tumorcells (2009)
  • Author(s): T. Hanafusa, T. Ono and E. Nakayama
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-02
• [Presentation] Simultaneous expression of multiple splice variants of PCDH11X/Y in tumor cells. (2009)
  • Author(s): T.Hanafusa, T.Ono, E.Nakayama
  • Organizer: 第68回日本癌学会学術総会
  • Place of Presentation: 横浜市
  • Year and Date: 2009-10-02
• [Presentation] Humoral immune response of cancer patients against new SEREX-defined antigen (2008)
  • Author(s): S. Domae, T. Ono(8名、2番目)
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-29
• [Presentation] Humoral immune response of cancer patients against new SEREX-defined antigen, CCDC62-2. (2008)
  • Author(s): S.Domae, T.Ono(8名、2番目)
  • Organizer: 第67回日本癌学会学術総会
  • Place of Presentation: 名古屋市
  • Year and Date: 2008-10-29
• [Presentation] SEREX法により同定したCCDC62-2に対する癌患者の液性免疫応答 (2008)
  • Author(s): 銅前昇平、小野俊朗(7名、2番目)
  • Organizer: 第12回基盤的癌免疫研究会総会
  • Place of Presentation: さいたま市
  • Year and Date: 2008-07-02
• [Presentation] Identification of a CD4 T-cell epitope on tumor rejection antigen RLakt in BALB/c radiation-leukemia RL male 1 (2008)
  • Author(s): K. Savas、上中明子(6名、4番目)
  • Organizer: 第12回基盤的癌免疫研究会総会
  • Place of Presentation: さいたま市
  • Year and Date: 2008-07-02
• [Presentation] Identification of a CD4 T-cell epitope on tumor rejection antigen RLakt in BALB/c radiation-leukemia RL male 1. (2008)
  • Author(s): K. Savas、上中明子(6名、4番目)
  • Organizer: 第12回基盤的癌免疫研究会総会
  • Place of Presentation: さいたま市
  • Year and Date: 2008-07-02
• [Presentation] SEREX法により同定したCCDC62-2に対する癌患者の液性免疫応答 (2008)
  • Author(s): 銅前 昇平, 他
  • Organizer: 第12回基盤的癌免疫研究会
  • Place of Presentation: 名古屋市
• [Presentation] SEREX法によるがん・精巣(CT)抗原CCDC62-2の同定とがん患者の液性免疫応答 (2008)
  • Author(s): 銅前 昇平, 他
  • Organizer: 第38回日本免疫学総会
  • Place of Presentation: 京都市
• [Patent(Industrial Property Rights)] 癌診断方法および癌診断キット (2012)
  • Inventor(s): 小野俊朗、中山睿一
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Filing Date: 2012-02-17
• [Patent(Industrial Property Rights)] Cancer Diagnostic Kit and Cancer Diagnostic Method (2012)
  • Inventor(s): Toshiro Ono, Eiichi Nakayama
  • Industrial Property Rights Holder: National University Corporation Okayama University
  • Acquisition Date: 2012-04-17
  • Overseas
• [Patent(Industrial Property Rights)] 癌診断キットおよび癌診断方法 (2010)
  • Inventor(s): 小野俊朗、中山睿一
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Industrial Property Number: 2009-532105
  • Acquisition Date: 2010-03-10
• [Patent(Industrial Property Rights)] Cancer Diagnostic Kit and Cancer Diagnostic Method (2010)
  • Inventor(s): Toshiro Ono, Eiichi Nakayama
  • Industrial Property Rights Holder: National University Corporation Okayama University
  • Filing Date: 2010-03-10
  • Acquisition Date: 2010-03-11
• [Patent(Industrial Property Rights)] 癌診断キットおよび癌診断方法 (2010)
  • Inventor(s): 小野俊朗中山睿一
  • Industrial Property Rights Holder: 国立大学法人岡山大学
  • Filing Date: 2010
  • Overseas
• [Patent(Industrial Property Rights)] 癌診断方法および癌診断キット (2009)
  • Inventor(s): 小野俊朗、中山睿一
  • Industrial Property Rights Holder: 岡山大学
  • Industrial Property Number: 2006-240787
  • Filing Date: 2009-07-14
• [Patent(Industrial Property Rights)] 癌診断キットおよび癌診断方法 (2008)
  • Inventor(s): 小野 俊朗 中山 睿一
  • Industrial Property Rights Holder: 岡山大学
  • Filing Date: 2008-07-18
  • Overseas
• [Patent(Industrial Property Rights)] Cancer Diagnostic Kit and Cancer Diagnostic Method
  • Inventor(s): 小野俊朗, 中山睿一
  • Industrial Property Rights Holder: National University Corporation Okayama University