| Project/Area Number |
20590576
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | The University of Tokushima |
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Principal Investigator |
UMENO Mayumi The University of Tokushima, 大学院・ヘルスバイオサイエンス研究部, 准教授 (00213497)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Youichi 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 准教授 (10363160)
NAKAHORI Yutaka 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (10172389)
YAMANO Shuji 徳島大学, 大学院・ヘルスバイオサイエンス研究部, 教授 (30166772)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 不妊症 / 遺伝子解析 |
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| Research Abstract |
We developed a rapid and simple system of detecting deletions on the Y chromosome related with male infertility using multiplex PCR and microchip electrophoresis. Moreover, direct PCR from whole blood was found to be able to use.
The mammalian SRY gene emerged by the gain-of-function mechanism ; cDNA of the DGCR8 gene was inserted upstream of an ancestral SOX3 gene on proto-sex chromosomes. As a result of Y chromosomalpolymorphism analysis, the Japanese men were classified in the haplogroupC, DE, O2b*, O2b1 and O3, and two genetic different populations (Jomon and Yayoi) exist in Japan.
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