Improved function of ketoamine oxidase for clinical examination reagent of diabetes by use of multiple approaches
| Project/Area Number |
20590579
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyushu University |
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Principal Investigator |
UEDA Tadashi Kyushu University, 薬学研究院, 教授 (90184928)
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| Co-Investigator(Kenkyū-buntansha) |
OHKURI Takatoshi 九州大学, 大学院・薬学研究院, 助教 (70346807)
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| Co-Investigator(Renkei-kenkyūsha) |
ABE Yoshito 九州大学, 大学院・薬学研究院, 准教授 (60315091)
SHIOI Seijiro 福岡大学, RIセンター実験施設, 助教 (80423531)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2008: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 糖尿病 / ケトアミンオキシダーゼ / アミノ酸改変 / X線結晶解析 / 安定化 / 酵素 / 機能向上 / 酵素の高機能化 / ジスルフィド結合 / タンパク質の安定化 / Gly-Pro配列 / グリコアルブミン |
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| Research Abstract |
For prevention of diabetes, we should control of the concentration of glucose in our vessels. Since the concentration of glucose is affected by foods, the concentration of glycated proteins in vessels often became used as an indicator of the average of concentration of glucose. The ketoamine oxidase is crucial for the evaluation of the concentration of glucose from the glycated proteins. To improve the function of the ketoamine oxidase, we prepared many ketoamine oxidase mutants but did not obtain ketoamine oxidase mutant with improved function. Then, in order to obtain the basis for mutation design, we tried to analyze the three-dimensional structure of the ketoamine oxidase in vain.
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Report
(4 results)
Research Products
(7 results)
