A new diagnostic approach of Alzheimer's Disease based on the conformational changes of Aβ in serum

• Project/Area Number: 20590592
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Tokushima Bunri University
• Principal Investigator: MATSUNAGA Yoichi Tokushima Bunri University, 薬学部, 教授 (80239053)
• Co-Investigator(Kenkyū-buntansha): YAMADA Tatsuo 福岡大学, 医学部, 教授 (50142211) ; SUENAGA Midori 徳島文理大学, 薬学部, 助教 (00389181)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: アルツハイマー病 / アミロイド蛋白 / 加温 / 蛋白立体構造変化 / 血清診断 / ベータアミロイド / ブレーカーペプタイド / 記憶障害 / 血清アミロイド蛋白濃度 / アミロイド蛋白凝集抑制 / アミロイドベータ蛋白 / 診断法 / 立体構造変化 / 患者血清 / 温熱変化 / ブレーカーペプチド / アミロイド蛋白凝集 / アミロイドベータ / 血清診断法 / 蛋白質加温凝集

Research Abstract

We clarified the conformational changes of amyloid protein in serum between from normal and Alzheimer's patients to the temperature within physiological limits. The former exhibited the sensitivity to temperature and the latter did not exhibit the sensitivity to temperature. The present study suggest a new approach to the diagnostics of Alzheimer's disease with serum.

Research Products

• [Journal Article] Effects of 8-residue beta sheet breaker peptides on Aged Aβ40-Induced memory impairment and Aβ40 expression in rat brain and serum following intraamygdaloid injection. (2010)
  • Author(s): Hatip F F, Hatip-Al-Khatib I, Y.Matsunaga, M.Suenaga, N.Sen
  • Journal Title: Curr Alzheimer Res 7(7) Pages: 602-614
• [Journal Article] Alcohol drinking and risk of Parkinson's disease : a case-control study in Japan. (2010)
  • Author(s): W.Fukushima, Y.Miyake, K.Tanaka, S.Sasaki, C.Kiyohara, Y.Tsuboi, T.Yamada, T.Oeda, T.Miki, N.Kawamura, N.Sakae, H.Fukuyama, Y.Hirota, M.Nagai, the Fukuoka Kinki Parkinson's Disease Study Group
  • Journal Title: BMC Neurology 10 Pages: 111-111
• [Journal Article] Active and passive smoking and risk of Parkinson's disease. (2010)
  • Author(s): K.Tanaka, Y.Miyake, W.Fukushima, S.Sasaki, C.Kiyohara, Y.Tsuboi, T.Yamada, T.Oeda, T.Miki, N.Kawamura, N.Sakae, H.Fukuyama, Y.Hirota, M.Nagai, the Fukuoka Kinki Parkinson's Disease Study Group
  • Journal Title: Acta.Neurol.Scand. 122 Pages: 377-382
• [Journal Article] Dietary intake of folate, vitamin B6, vitamin B12 and riboflavin and risk of Parkinson's disease : a case-control study in Japan. (2010)
  • Author(s): K.Murakami, Y.Miyake, S.Sasaki, K.Tanaka, W.Fukushima, C.Kiyohara, Y.Tsuboi, T.Yamada, T.Oeda, T.Miki, N.Kawamura, N.Sakae, H.Fukuyama, Y.Hirota, M.Nagai, Fukuoka Kinki Parkinson's disease Study Group
  • Journal Title: Br.J.Nutr 104 Pages: 757-764
• [Journal Article] Case-control study of risk of Parkinson's disease in relation to hypertension, hypercholesterolemia, and diabetes in Japan. (2010)
  • Author(s): Y.Miyake, T.Tanaka, W.Fukushima, S. Sasaki, C.Kiyohara, Y.Tsuboi, T.Yamada, T.Oeda, T.Miki, N.Kawamura, N.Sakae, H. Fukuyama, Y.Hirota, M.Nagai, the Fukuoka Kinki Parkinson's Disease Study Group
  • Journal Title: J Neurol Sci. 293 Pages: 82-86
• [Journal Article] Less protease-resistant PrP in a patient with sporadic CJD treated with intraventricular pentosan polysulpfate. (2010)
  • Author(s): T.Terada, Y.Tsuboi, T.Obi, K.Doh-ura, S.Murayama, T.Kitamoto, T.Yamada, K.Mizoguchi
  • Journal Title: Acta.Neurol.Scand. 121 Pages: 127-130
• [Journal Article] 疫学と予防5)Alzheimer病の危険因子とライフスタイルの改善による予防効果 (2010)
  • Author(s): 合馬慎二、山田達夫
  • Journal Title: 神経内科 72(6) Pages: 28-33
• [Journal Article] 認知症予防のための安心院プロジェクト9年間の歩み (2010)
  • Author(s): 吉田香織、大久保みゆき、山田達夫
  • Journal Title: 地域保健 10(6) Pages: 74-81
• [Journal Article] 治療・ケア認知症ケア (2010)
  • Author(s): 福原藍加、山田達夫
  • Journal Title: Clinical Neuroscience 28(9) Pages: 1052-1053
• [Journal Article] 社会的・積極レジャー活動を中心とした非薬物療法によるMild Cognitive Impariment(MCI)からの認知症への進行予防-安心院プロジェクト (2010)
  • Author(s): 山田達夫
  • Journal Title: Geriat.Med. 48(5) Pages: 653-656
• [Journal Article] Q&A-神経科学の素朴な質問 年をとっても認知症にならない人がいるのはなぜですか? (2010)
  • Author(s): 山田達夫、玉木慶子
  • Journal Title: Clinical Neuroscience 28 Pages: 463-463
• [Journal Article] Effects of 8-residue beta sheet breaker peptides on aged A-beta40-induced memory impairment and A-beta40 expression in rat brain and serm following intraamygdaloid injection (2010)
  • Author(s): F.Hatip, Y I.Hatip, Matsunaga, M.Suenaga, N.Sen
  • Journal Title: Current Alzheimer's Research Volume: 7 Pages: 602-614
  • Peer Reviewed
• [Journal Article] Reversal of temperature-induced conformational changes in the Amyloid-beta peptide, Aβ40, by the β-sheet breaker peptides 16-23 and 17-24. (2009)
  • Author(s): Hatip B.F, M.Suenaga, T.Yamada, Y.Matsunaga
  • Journal Title: Br J Pharmacol 158 Pages: 1165-1172
• [Journal Article] Continuous intraventricular infusion of pentosan polysulphate : clinical trial against prion diseases. (2009)
  • Author(s): Y.Tsuboi, K.Doh-ura, T.Yamada
  • Journal Title: Neuropathology 29(5) Pages: 632-636
• [Journal Article] 軽度認知障害者への非薬物的介入効果 (2009)
  • Author(s): 長愛、山田達夫、鐘ヶ江秀樹、浜崎裕子、中島七海、平原一寿
  • Journal Title: 地域保健 40(12) Pages: 64-69
• [Journal Article] 認知症医療によるアルツハイマー型認知症の本人および介護者の包括的健康関連QOL指標の変化 (2009)
  • Author(s): 八森淳、安田朝子、本間昭、朝田隆、池田学、河野禎之、稲葉百合子、木之下徹、内海久美子、奥村歩、川嶋乃里子、川畑信也、繁田雅弘、繁信和恵、高橋智、田北昌史、玉井顕、長田乾、橋本衛、平井茂夫、藤沢嘉勝、水上勝義、山田達夫、小坂憲司
  • Journal Title: 老年精神医学雑誌 20(9) Pages: 1009-1021
• [Journal Article] ドネペジル塩酸塩によるアルツハイマー型認知症患者とその家族の包括的健康関連QOL指標の変化に関する研究 (2009)
  • Author(s): 八森淳、河野禎之、本間昭、朝田隆、池田学、安田朝子、稲葉百合子、木之下徹、内海久美子、奥村歩、川嶋乃里子、川畑信也、繁田雅弘、繁信和恵、高橋智、田北昌史、玉井顕、長田乾、橋本衛、平井茂夫、藤沢嘉勝、水上勝義、山田達夫、小坂憲司
  • Journal Title: 老年精神医学雑誌 20(9) Pages: 997-1008
• [Journal Article] MCIの人に対するエンパワメント・ツールとしてのまちづくりワークショップの提案 (2009)
  • Author(s): 浜崎裕子、山田達夫、中島七海、平原一寿
  • Journal Title: 地域保健 40(1) Pages: 48-61
• [Journal Article] Reversal of temperature-induced conformational changes in the amyloid-beta peptides, A-beta40, by the beta-sheet breaker peptides 16-23 and 17-24 (2009)
  • Author(s): Funda Bolukbasi Hatip, Midori Suenaga, Tatsuo Yamada, Yoichi Matsunaga
  • Journal Title: British Journal of Pharmacology 158 Pages: 1165-1172
  • Peer Reviewed
• [Journal Article] Reversal of temperature-induced conformational changes in A-beta40 by beta-sheet breakers pentides 16-23/17-24 (2009)
  • Author(s): Funda F. Bolukbasi Hatip
  • Journal Title: British Journal of pharmacology (In press)
  • Peer Reviewed
• [Journal Article] Differentiation of early-stage Alzheimer's Disease from other types of dementia using brain perfusion SPECT with eZIS analysis. (2008)
  • Author(s): M.Waragai, S.Mizumura, T.Yamada, H.Matsuda
  • Journal Title: Dement.Geriatr.Cogn.Disord. 26 Pages: 547-555
• [Journal Article] ATP-binding cassette transporter G4 is highly expressed in microglia in Alzheimer's brain. (2008)
  • Author(s): Y.Uehara, T.Yamada, Y.Baba, SI.Miura, S.Abe, K.Kitajima, M.Higuchi, T.Iwamoto, K.Saku
  • Journal Title: Brain Res 27(1217C) Pages: 239-246
• [Journal Article] The Japanese MCI Screen for Early Detection of Alzheimer's Disease and Related Disorders. (2008)
  • Author(s): Ai.Cho, M.Sugimura, S.Nakano, T.Yamada
  • Journal Title: Am J Alzheimer's Disease and Other Dementias 23(2) Pages: 162-166
• [Journal Article] CogHealthの信頼性、妥当性、外挿可能性に関する検討 (2008)
  • Author(s): 緒方真一、山田達夫、本橋伸高、山縣然太郎、天野恵子、篠遠仁、吉井文均、石井敏仁、田中司朗
  • Journal Title: 認知神経学 10(1) Pages: 119-129
  • NAID: 40016029101
• [Journal Article] 特集 高齢者認知症の知識と理解 認知症の予防 栄養と生活習慣 (2008)
  • Author(s): 山田達夫
  • Journal Title: 臨床と研究 85(4) Pages: 67-69
• [Journal Article] 特集 今日のアルツハイマー病研究大手が掛った根治薬 アルツハイマー病臨床研究の最前線 予防 (2008)
  • Author(s): 杉村美佳、山田達夫
  • Journal Title: Current Therapy 26(4) Pages: 59-63
• [Journal Article] Specific reactivity of mild severe Alzheimer's disease patient's sera to antibody against A-betal-40 epitope 17-21 (2007)
  • Author(s): Funda Bolukbasi Hatip, Yoichi Matsunaga, Tatsuo Yamada
  • Journal Title: Acta Neurologica Acandinavica 117 Pages: 404-408
  • Peer Reviewed
• [Journal Article] Reversal of temperature-induced conformational changes in the amyloid-beta peptide, Aβ40, by the β-sheet breaker peptides 16-23 and 17-24.
  • Author(s): F.Hatip, M.Suenaga, T.Yamada, Y.Matsunaga
• [Presentation] 高濃度Vitamine D2によるbeta-amyloidの凝集促進 (2011)
  • Author(s): 末永みどり
  • Organizer: 第21回日本老年病学会、九州支部会
  • Place of Presentation: 福岡市
  • Year and Date: 2011-03-05
• [Presentation] 高濃度Vitamine D2によるベータアミロイド凝の集促進 (2011)
  • Author(s): 末永みどり、松永洋一、合馬信二、山田達夫
  • Organizer: 日本老年病学会
  • Place of Presentation: 福岡市
• [Presentation] Reversal of temperature-induced conformational changes in A-beta40 by beta-breaker peptides 16-23 and 17-24. (2010)
  • Author(s): M.Suenaga, T.Yamada, Y.Matsunaga
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸市
• [Presentation] β-sheet breaker peptides 16-23及び17-24による温度誘導性Aβ40構造変化の抑制 (2009)
  • Author(s): 末永みどり、松永洋一
  • Organizer: 第82回日本生化学会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-10-21
• [Presentation] アルツハイマー病及びメタボリツク症候群に関与するApoE分解酵素の検索 (2008)
  • Author(s): 末永みどり
  • Organizer: 第81回日本生化学会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12
• [Remarks] ホームページ等