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Study on prevalence of PVL-positive Staphylococcus aureus and MRSA with the use of novel molecular epidemiologic methods

Research Project

Project/Area Number 20590608
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hygiene
Research InstitutionSapporo Medical University

Principal Investigator

KOBAYASHI Nobumichi  Sapporo Medical University, 医学部, 教授 (80186759)

Co-Investigator(Kenkyū-buntansha) ISHINO Masaho  札幌医科大学, 医学部, 准教授 (30232325)
SUMI Ayako  札幌医科大学, 医学部, 講師 (10363699)
NAGASHIMA Mshigeo  札幌医科大学, 医学部, 助教 (60433116)
URUSHIBARA Noriko  札幌医科大学, 医学部, 助教 (80396308)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords黄色ブドウ球菌 / MRSA / PVL / 遺伝子型 / スタフィロコアグラーゼ / MLST / コアグラーゼ型 / 市中感染
Research Abstract

Prevalence and molecular epidemiologic characteristics of methicillin-resistant S.aureus (MRSA) were analyzed for numerous Staphylococcus aureus isolates derived from outpatients in hospitals in Hokkaido. As a result, several MRSA strains (genotypes ST6, ST59) which harbor the Panton-Valentine Leukocidin (PVL) gene typical to CA-MRSA, and the genetic element ACME unique to USA300 CA-MRSA clone predominating in USA,were identified, suggesting the wide distribution of these MRSA to the community.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (23 results)

All 2011 2010 2009 2008

All Journal Article (12 results) (of which Peer Reviewed: 8 results) Presentation (11 results)

  • [Journal Article] Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Hokkaido, northern main island of Japan : identification of ST6 and ST59 PVL-positive CA-MRSA.2011

    • Author(s)
      Kawaguchiya M, UrushibaraN, Kuwahara O, Ito M, Mise K, Kobayashi N.
    • Journal Title

      Microbial Drug Resistance in press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Analysis of staphylococcal cassette chromosome mec (SCCmec) in Staphylococcus haemolyticus and Staphylococcus sciuri : identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7).2011

    • Author(s)
      Urushibara N, Paul SK, Hossain MA, Kawaguchiya M, Kobayashi N.
    • Journal Title

      Microbial Drug Resistance in press

    • Related Report
      2010 Final Research Report
  • [Journal Article] Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Hokkaido, northern main island of Japan : identification of ST6 and ST59 PVL-positive CA-MRSA.2011

    • Author(s)
      Kawaguchiya M., et al.
    • Journal Title

      Microbial Drug Resistance

      Volume: 17(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of staphylococcal cassette chromosome mec (SCCmec) in Staphylococcus haemolyticus and Staphylococcus sciurr. identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7).2011

    • Author(s)
      Urushibara N., et al.
    • Journal Title

      Microbial Drug Resistance

      Volume: 17(印刷中)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification of Staphylocoagulase genotypes I-X and discrimination of type IV and V subtypes by multiplex PCR assay for clinical isolates of Staphylococcus aureus.2010

    • Author(s)
      Hirose M, Kobayashi N, Ghosh S, Paul SK, Shen T, Urushibara N, Kawaguchiya M, Shinagawa M, Watanabe N.
    • Journal Title

      Jpn J Infect Dis 63

      Pages: 257-263

    • Related Report
      2010 Final Research Report
  • [Journal Article] Identification of Staphylocoagulase genotypes I-X and discrimination of type IV and V subtypes by multiplex PCR assay for clinical isolates of Staphylococcus aureus.2010

    • Author(s)
      Hirose M., et al.
    • Journal Title

      Japanese Journal of Infectious Diseases

      Volume: 63 Pages: 257-263

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of enterococci harboring high-level gentamicin resistance genes aac(6')-Ie-aph(2")-Ia or aph(2")-Ie in a Japanese hospital2009

    • Author(s)
      Watanabe S, et al.
    • Journal Title

      Microbial Drug Resistance 15

      Pages: 185-194

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular epidemiologic analysis of Enterococcus faecalis isolates in Cuba by multilocus sequence typing2009

    • Author(s)
      Quinones D, et al.
    • Journal Title

      Microbial Drug Resistance 15

      Pages: 287-293

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of the low-level vancomycin resistance gene vanC-2/vanC-3 and identification of a novel vanC subtype (vanC-4) in Enterococcus casseliflavus.2009

    • Author(s)
      Watanabe S, et al.
    • Journal Title

      Microbial Drug Resistance 15

      Pages: 1-9

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh.2008

    • Author(s)
      Afroz S, Kobayashi N, Nagashima S, Alam MM, Hossain ABMB, Rahman A, Islam MR, Lutfor AB, Muazzam N, Khan MAH, Paul SK, Shamsuzzaman AKM, Mahmud MC, Musa AKM, Hossain MA.
    • Journal Title

      Jpn J Infect Dis 61

      Pages: 393-396

    • NAID

      40016249990

    • Related Report
      2010 Final Research Report
  • [Journal Article] Diversity of staphylocoagulase and identification of nove1 variants of staphylocoagulase gene in Staphylococcus aureus.2008

    • Author(s)
      Kinoshita M, et al.
    • Journal Title

      Microbiology and Immunology 52

      Pages: 334-348

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh.2008

    • Author(s)
      Afroz S, et al.
    • Journal Title

      Japanese Journal of Infectious Diseases 61

      Pages: 393-396

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Presentation] Novel staphylococcal cassette chromosome mec harboring two copies of mecA and two copies of ccrC genes found in a Bangladeshi clinical strain of Staphylococcus haemolyticus.2010

    • Author(s)
      Urushibara N, Kawaguchiya M, Paul SK, Kobayashi N
    • Organizer
      12th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      シンガポール
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] Genetic characteristics and distribution of virulence determinants in Staphylococcus aureus isolates in Myanmar.2010

    • Author(s)
      Kobayashi N, Nwe KM, Aung TS, Urushibara N, Kawaguchiya M, Ghosh S.
    • Organizer
      12th Western Pacific Congress on Chemotherapy and Infectious Diseases
    • Place of Presentation
      シンガポール
    • Year and Date
      2010-12-04
    • Related Report
      2010 Annual Research Report 2010 Final Research Report
  • [Presentation] バングラデシュにて分離されたコアグラーゼ陰性ブドウ球菌における新規SCCmec型2010

    • Author(s)
      漆原範子、川口谷充代、S.K.Paul、小林宣道
    • Organizer
      第80回日本衛生学会総会
    • Place of Presentation
      仙台
    • Year and Date
      2010-05-11
    • Related Report
      2010 Final Research Report
  • [Presentation] ミャンマーにおいて検出された黄色ブドウ球菌の遺伝学的性状と各種病原因子遺伝子の分布2010

    • Author(s)
      小林宣道、漆原範子、川口谷充代
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] 北海道における市中感染型MRSAの分布状況と疫学的特徴2010

    • Author(s)
      川口谷充代、漆原範子、桑原理、伊藤政彦、小林宣道
    • Organizer
      第83回日本細菌学会総会
    • Place of Presentation
      横浜
    • Year and Date
      2010-03-28
    • Related Report
      2010 Final Research Report 2009 Annual Research Report
  • [Presentation] Genetic diversity of enterococci harboring high-level gentamicin resistance genes aac(6')-Ie-aph(2")-Ia or aph(2")-Ie in a Japanese hospital2010

    • Author(s)
      小林宣道, 渡辺祥二郎, 長嶋茂雄, D.キニョネス, 漆原範子
    • Organizer
      第14回国際感染症学会
    • Place of Presentation
      米国・マイアミ
    • Year and Date
      2010-03-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Developing of genetic typing method to assign staphylocoagulase(SC)serotype I-X and two SC genotypes for Staphylococcus aureus2010

    • Author(s)
      小林宣道, S.ゴッシュ、廣瀬弥奈, S.K.パウル, 漆原範子
    • Organizer
      第14回国際感染症学会
    • Place of Presentation
      米国・マイアミ
    • Year and Date
      2010-03-12
    • Related Report
      2009 Annual Research Report
  • [Presentation] Genetic diversity of enterococci harboring high-level gentamicin resistance genes aac(6')-Ie-aph(2")-Ia or aph(2")-Ie in a Japanese hospital.2010

    • Author(s)
      Kobayashi N, Watanabe S, Nagashima S, Quinones D, Urushibara N
    • Organizer
      14th International Congress on Infectious Diseases.
    • Place of Presentation
      マイアミ、米国
    • Year and Date
      2010-03-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Developing of genetic typing method to assign staphylocoagulase (SC) serotype I-X and two SC genotypes for Staphylococcus aureus.2010

    • Author(s)
      Kobayashi N, Ghosh S, Hirose M, Paul SK, Urushibara N, Kawaguchiya M
    • Organizer
      14th International Congress on Infectious Diseases.
    • Place of Presentation
      マイアミ、米国
    • Year and Date
      2010-03-10
    • Related Report
      2010 Final Research Report
  • [Presentation] PCRによる黄色ブドウ球菌のコアグラーゼ型・コアグラーゼ遺伝子型同定法の確立2009

    • Author(s)
      小林宣道、渡辺祥二郎
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2010 Final Research Report
  • [Presentation] PCRによる黄色ブドウ球菌のコアグラーゼ型・コアグラーゼ遺伝子型同定法の確立.2009

    • Author(s)
      小林宣道
    • Organizer
      第82回日本細菌学会総会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-03-13
    • Related Report
      2008 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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