Study on prevalence of PVL-positive Staphylococcus aureus and MRSA with the use of novel molecular epidemiologic methods

• Project/Area Number: 20590608
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Hygiene
• Research Institution: Sapporo Medical University
• Principal Investigator: KOBAYASHI Nobumichi Sapporo Medical University, 医学部, 教授 (80186759)
• Co-Investigator(Kenkyū-buntansha): ISHINO Masaho 札幌医科大学, 医学部, 准教授 (30232325) ; SUMI Ayako 札幌医科大学, 医学部, 講師 (10363699) ; NAGASHIMA Mshigeo 札幌医科大学, 医学部, 助教 (60433116) ; URUSHIBARA Noriko 札幌医科大学, 医学部, 助教 (80396308)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2008: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 黄色ブドウ球菌 / MRSA / PVL / 遺伝子型 / スタフィロコアグラーゼ / MLST / コアグラーゼ型 / 市中感染

Research Abstract

Prevalence and molecular epidemiologic characteristics of methicillin-resistant S.aureus (MRSA) were analyzed for numerous Staphylococcus aureus isolates derived from outpatients in hospitals in Hokkaido. As a result, several MRSA strains (genotypes ST6, ST59) which harbor the Panton-Valentine Leukocidin (PVL) gene typical to CA-MRSA, and the genetic element ACME unique to USA300 CA-MRSA clone predominating in USA,were identified, suggesting the wide distribution of these MRSA to the community.

Research Products

• [Journal Article] Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Hokkaido, northern main island of Japan : identification of ST6 and ST59 PVL-positive CA-MRSA. (2011)
  • Author(s): Kawaguchiya M, UrushibaraN, Kuwahara O, Ito M, Mise K, Kobayashi N.
  • Journal Title: Microbial Drug Resistance in press
• [Journal Article] Analysis of staphylococcal cassette chromosome mec (SCCmec) in Staphylococcus haemolyticus and Staphylococcus sciuri : identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7). (2011)
  • Author(s): Urushibara N, Paul SK, Hossain MA, Kawaguchiya M, Kobayashi N.
  • Journal Title: Microbial Drug Resistance in press
• [Journal Article] Molecular characteristics of community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) in Hokkaido, northern main island of Japan : identification of ST6 and ST59 PVL-positive CA-MRSA. (2011)
  • Author(s): Kawaguchiya M., et al.
  • Journal Title: Microbial Drug Resistance Volume: 17(印刷中)
  • Peer Reviewed
• [Journal Article] Analysis of staphylococcal cassette chromosome mec (SCCmec) in Staphylococcus haemolyticus and Staphylococcus sciurr. identification of a novel ccr gene complex with a newly identified ccrA allotype (ccrA7). (2011)
  • Author(s): Urushibara N., et al.
  • Journal Title: Microbial Drug Resistance Volume: 17(印刷中)
  • Peer Reviewed
• [Journal Article] Identification of Staphylocoagulase genotypes I-X and discrimination of type IV and V subtypes by multiplex PCR assay for clinical isolates of Staphylococcus aureus. (2010)
  • Author(s): Hirose M, Kobayashi N, Ghosh S, Paul SK, Shen T, Urushibara N, Kawaguchiya M, Shinagawa M, Watanabe N.
  • Journal Title: Jpn J Infect Dis 63 Pages: 257-263
• [Journal Article] Identification of Staphylocoagulase genotypes I-X and discrimination of type IV and V subtypes by multiplex PCR assay for clinical isolates of Staphylococcus aureus. (2010)
  • Author(s): Hirose M., et al.
  • Journal Title: Japanese Journal of Infectious Diseases Volume: 63 Pages: 257-263
  • Peer Reviewed
• [Journal Article] Genetic diversity of enterococci harboring high-level gentamicin resistance genes aac(6')-Ie-aph(2")-Ia or aph(2")-Ie in a Japanese hospital (2009)
  • Author(s): Watanabe S, et al.
  • Journal Title: Microbial Drug Resistance 15 Pages: 185-194
  • Peer Reviewed
• [Journal Article] Molecular epidemiologic analysis of Enterococcus faecalis isolates in Cuba by multilocus sequence typing (2009)
  • Author(s): Quinones D, et al.
  • Journal Title: Microbial Drug Resistance 15 Pages: 287-293
  • Peer Reviewed
• [Journal Article] Genetic diversity of the low-level vancomycin resistance gene vanC-2/vanC-3 and identification of a novel vanC subtype (vanC-4) in Enterococcus casseliflavus. (2009)
  • Author(s): Watanabe S, et al.
  • Journal Title: Microbial Drug Resistance 15 Pages: 1-9
  • Peer Reviewed
• [Journal Article] Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh. (2008)
  • Author(s): Afroz S, Kobayashi N, Nagashima S, Alam MM, Hossain ABMB, Rahman A, Islam MR, Lutfor AB, Muazzam N, Khan MAH, Paul SK, Shamsuzzaman AKM, Mahmud MC, Musa AKM, Hossain MA.
  • Journal Title: Jpn J Infect Dis 61 Pages: 393-396
  • NAID: 40016249990
• [Journal Article] Diversity of staphylocoagulase and identification of nove1 variants of staphylocoagulase gene in Staphylococcus aureus. (2008)
  • Author(s): Kinoshita M, et al.
  • Journal Title: Microbiology and Immunology 52 Pages: 334-348
  • Peer Reviewed
• [Journal Article] Genetic characterization of Staphylococcus aureus isolates carrying Panton-Valentine leukocidin genes in Bangladesh. (2008)
  • Author(s): Afroz S, et al.
  • Journal Title: Japanese Journal of Infectious Diseases 61 Pages: 393-396
  • Peer Reviewed
• [Presentation] Novel staphylococcal cassette chromosome mec harboring two copies of mecA and two copies of ccrC genes found in a Bangladeshi clinical strain of Staphylococcus haemolyticus. (2010)
  • Author(s): Urushibara N, Kawaguchiya M, Paul SK, Kobayashi N
  • Organizer: 12th Western Pacific Congress on Chemotherapy and Infectious Diseases
  • Place of Presentation: シンガポール
  • Year and Date: 2010-12-04
• [Presentation] Genetic characteristics and distribution of virulence determinants in Staphylococcus aureus isolates in Myanmar. (2010)
  • Author(s): Kobayashi N, Nwe KM, Aung TS, Urushibara N, Kawaguchiya M, Ghosh S.
  • Organizer: 12th Western Pacific Congress on Chemotherapy and Infectious Diseases
  • Place of Presentation: シンガポール
  • Year and Date: 2010-12-04
• [Presentation] バングラデシュにて分離されたコアグラーゼ陰性ブドウ球菌における新規SCCmec型 (2010)
  • Author(s): 漆原範子、川口谷充代、S.K.Paul、小林宣道
  • Organizer: 第80回日本衛生学会総会
  • Place of Presentation: 仙台
  • Year and Date: 2010-05-11
• [Presentation] ミャンマーにおいて検出された黄色ブドウ球菌の遺伝学的性状と各種病原因子遺伝子の分布 (2010)
  • Author(s): 小林宣道、漆原範子、川口谷充代
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] 北海道における市中感染型MRSAの分布状況と疫学的特徴 (2010)
  • Author(s): 川口谷充代、漆原範子、桑原理、伊藤政彦、小林宣道
  • Organizer: 第83回日本細菌学会総会
  • Place of Presentation: 横浜
  • Year and Date: 2010-03-28
• [Presentation] Genetic diversity of enterococci harboring high-level gentamicin resistance genes aac(6')-Ie-aph(2")-Ia or aph(2")-Ie in a Japanese hospital (2010)
  • Author(s): 小林宣道, 渡辺祥二郎, 長嶋茂雄, D.キニョネス, 漆原範子
  • Organizer: 第14回国際感染症学会
  • Place of Presentation: 米国・マイアミ
  • Year and Date: 2010-03-12
• [Presentation] Developing of genetic typing method to assign staphylocoagulase(SC)serotype I-X and two SC genotypes for Staphylococcus aureus (2010)
  • Author(s): 小林宣道, S.ゴッシュ、廣瀬弥奈, S.K.パウル, 漆原範子
  • Organizer: 第14回国際感染症学会
  • Place of Presentation: 米国・マイアミ
  • Year and Date: 2010-03-12
• [Presentation] Genetic diversity of enterococci harboring high-level gentamicin resistance genes aac(6')-Ie-aph(2")-Ia or aph(2")-Ie in a Japanese hospital. (2010)
  • Author(s): Kobayashi N, Watanabe S, Nagashima S, Quinones D, Urushibara N
  • Organizer: 14th International Congress on Infectious Diseases.
  • Place of Presentation: マイアミ、米国
  • Year and Date: 2010-03-10
• [Presentation] Developing of genetic typing method to assign staphylocoagulase (SC) serotype I-X and two SC genotypes for Staphylococcus aureus. (2010)
  • Author(s): Kobayashi N, Ghosh S, Hirose M, Paul SK, Urushibara N, Kawaguchiya M
  • Organizer: 14th International Congress on Infectious Diseases.
  • Place of Presentation: マイアミ、米国
  • Year and Date: 2010-03-10
• [Presentation] PCRによる黄色ブドウ球菌のコアグラーゼ型・コアグラーゼ遺伝子型同定法の確立 (2009)
  • Author(s): 小林宣道、渡辺祥二郎
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] PCRによる黄色ブドウ球菌のコアグラーゼ型・コアグラーゼ遺伝子型同定法の確立. (2009)
  • Author(s): 小林宣道
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13