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A novel method of diagnosis for acute myocardial infarction

Research Project

Project/Area Number 20590679
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionNiigata University (2009-2011)
Shimane University (2008)

Principal Investigator

TAKATSUKA Hisakazu  新潟大学, 医歯学系, 准教授 (40242490)

Co-Investigator(Kenkyū-buntansha) TAKESHITA Haruo  島根大学, 医学部, 教授 (90292599)
FUJIHARA Junko  島根大学, 医学部, 助教 (20346381)
Co-Investigator(Renkei-kenkyūsha) YASUDA Toshihiro  福井大学, 医学部, 教授 (80175645)
Project Period (FY) 2008 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords心筋梗塞 / DNase I / 司法解剖 / 法医学 / DNaseI / NT-proBNP / DNaseI活性 / BNP / DNase活性 / 遺伝的多型 / 急性心筋梗塞 / 法医病理学 / 病理組織学的診断 / 生化学
Research Abstract

The aim of this study was confirm a molecular basis for utilization of Dnase I as a diagnostic marker death due to acute myocardial infarction; (1) SNP of Dnase I exon8, A2317G is the same tendency of Asians, and this SNP affect to heat sensitivity and optimum pH. (2) Detect of Dnase I activity within 10 minutes using microchip electrophoresis. (3) Unable to detect cytokines mRNA of postmortem cardiac tissue. (4) Unable to detect NT-pro-BNP using postmortem blood for novel diagnostic method. (5) SNP of Dnase I, type of 1-2 is the risk factor for myocardial infarction.

Report

(6 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report   Self-evaluation Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (24 results)

All 2011 2010 2009 2008

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (9 results) Book (1 results)

  • [Journal Article] Functional and genetic survey of all known the SNPs within human deoxyribonuclease I gene in wide-ranging ethnic groups2011

    • Author(s)
      Fujihara J, Ueki M, Yasuda T, Iida R, Soejima M, Koda Y, Kimura-Kataoka K,Kato H, Panduro A, Tongu M, Takeshita H
    • Journal Title

      DNA and Cell Biol.

      Volume: 30(4) Issue: 4 Pages: 205-217

    • DOI

      10.1089/dna.2010.1120

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradationRapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation2011

    • Author(s)
      Fujihara J, Tabuchi M, Yasuda T, Fujita Y, Takeshita H
    • Journal Title

      Anal Biochem

      Volume: 413(1) Issue: 1 Pages: 78-79

    • DOI

      10.1016/j.ab.2011.02.011

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degradation2011

    • Author(s)
      Fujihara J、Tabuchi M、Yasuda T、Fujita Y、Takeshita H
    • Journal Title

      Anal Biochem 413

      Pages: 78-79

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Rapid measurement of deoxyribonuclease I activity with the use of microchip electrophoresis based on DNA degeneration.2011

    • Author(s)
      Fujihara J, ら
    • Journal Title

      Analytical Biochemistry

      Volume: 413 Pages: 78-79

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A biochemical and genetic study on all non-synonymous single nucleotide polymorphisms of the gene encoding human deoxyribonuclease I potentially relevant to autoimmunity2010

    • Author(s)
      Yasuda T, Ueki M, Takeshita H, Fujihara J, Kimura-Kataoka K, Iida R, Tsubota E, Soejima M, Koda Y, Kato H, Panduro A
    • Journal Title

      Int J Biochem Cell Biol.

      Volume: 42(7) Issue: 7 Pages: 1216-1225

    • DOI

      10.1016/j.biocel.2010.04.012

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Global analysis of single nucleotide polymorphisms in the exons of human deoxyribonuclease I-like 1 and 2 genes2010

    • Author(s)
      Fujihara J, Yasuda T, Iida R, Kimura-Kataoka K, Soejima M, Koda Y, Kato H, Panduro A, Yuasa I, Takeshita H
    • Journal Title

      Electrophoresis

      Volume: 31(21) Issue: 21 Pages: 3552-3557

    • DOI

      10.1002/elps.201000319

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences2009

    • Author(s)
      Takeshita H , Soejima M, Koda Y, Yasuda T, Takatsuka H, Fujihara J
    • Journal Title

      Clin Chem Lab Med

      Volume: 47(1) Issue: 1 Pages: 51-55

    • DOI

      10.1515/cclm.2009.002

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Serum deoxyribonuclease I can be used as a useful marker for diagnosis of death due to ischemic heart disease2009

    • Author(s)
      Yasuda T, Iida R, Kawai Y, Nakajima T, Kominato Y, Fujihara J, Takeshita H
    • Journal Title

      Legal Med

      Volume: 11 Pages: S213-S215

    • DOI

      10.1016/j.legalmed.2009.01.092

    • NAID

      10027262728

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Gln222Arg (A2317G) polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences2009

    • Author(s)
      Takeshita H、Soejima M、Koda Y、Yasuda T、Takatsuka H、Fujihara J
    • Journal Title

      Clin Chem Lab Med 47

      Pages: 51-55

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Journal Article] Gln222Arg(A2317G)polymorphism in the deoxyribonuclease I gene exhibits ethnic and functional differences.2009

    • Author(s)
      Takeshita H, ら
    • Journal Title

      Clin Chem Lab Med 47

      Pages: 51-55

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] HPLC determination of chlorhexidine(CHX)in whole blood by solid phase extraction and kinetics following an intravenous infusion in rats.2009

    • Author(s)
      Xue Y, ら
    • Journal Title

      J Anal Toxicol 33

      Pages: 85-91

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytochrome P450 1A1, glutathione S-transferases M1 and T1 polymorphisms in Ovambos and Mongolians.2009

    • Author(s)
      Fujihara J, ら
    • Journal Title

      Legal Med 11

      Pages: 408-410

    • NAID

      10027263340

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Extremely high prevalence of DNASE1*1 allele in African populations2008

    • Author(s)
      Takeshita H, Fujihara J, Soejima M, Koda Y, Yasuda T, Nakajima T
    • Journal Title

      Cell Biochem Funct

      Volume: 26 Issue: 2 Pages: 151-153

    • DOI

      10.1002/cbf.1414

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] A case of sudden death after gingival injection of a therapeutic dose of lidocaine2008

    • Author(s)
      Hasegawa M、Fujihara J、Takatsuka H、Chung HC
    • Journal Title

      Forensic Toxicol 26

      Pages: 41-44

    • Related Report
      2010 Self-evaluation Report
    • Peer Reviewed
  • [Presentation] GISを用いた新潟県内の水死体の解析2011

    • Author(s)
      川井桂, ら
    • Organizer
      第96次日本法医学会学術全国集会
    • Place of Presentation
      福島県コラッセふくしま
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] 哺乳類DNase IにおけるN-グリコシド型糖鎖解2010

    • Author(s)
      藤原純子
    • Organizer
      第94次日本法医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] DNase I及びDNase II遺伝子exon内SNP検索:集団調査及び酵素活性に及ぼす変異の影響2010

    • Author(s)
      木村かおり
    • Organizer
      第94次日本法医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] サンドイッチELISA法による血清DNase I蛋白量の測定2010

    • Author(s)
      中島たみ子
    • Organizer
      第94次日本法医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2010-06-24
    • Related Report
      2011 Final Research Report
  • [Presentation] DNase I exon内非同義置換SNP検索:酵素性状に及ぼす置換の影響2009

    • Author(s)
      藤原純子
    • Organizer
      第26回日本法医学会中四国地方会
    • Place of Presentation
      山口
    • Year and Date
      2009-10-17
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] DNase II遺伝子exon内SNP検索:酵素活性に及ぼす変異の影響2009

    • Author(s)
      木村かおり
    • Organizer
      第26回日本法医学会中四国地方会
    • Place of Presentation
      山口
    • Year and Date
      2009-10-17
    • Related Report
      2011 Final Research Report 2010 Self-evaluation Report
  • [Presentation] DNA修復酵素(hOGGおよびXRCC1)多型における酸化ストレスとの相関2009

    • Author(s)
      竹下治男
    • Organizer
      第93次日本法医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-15
    • Related Report
      2011 Final Research Report
  • [Presentation] DNASE1 exon内SNP検索:アジア人においてDNase I Gln222Argのみが多型性を有する2009

    • Author(s)
      藤原純子
    • Organizer
      第93次日本法医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-14
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] DNASE I exon内SNP検索:アジア人においてDNase I Glu 222 Argのみが多型性を有する2009

    • Author(s)
      藤原純子, ら
    • Organizer
      第93次日本法医学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2009-05-14
    • Related Report
      2010 Self-evaluation Report
  • [Book] NA多型vol.162009

    • Author(s)
      藤原純子, 竹下治男, 高塚尚和, 安田年博, 植木美鈴, 飯田礼子, 河合康幸, 中島たみ子, 小湊慶彦, 副島美貴子, 神田芳郎
    • Publisher
      東洋書店
    • Related Report
      2011 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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