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STR genotyping of the DNA fragments from fingerprint-lines captured by using a laser microdissection method

Research Project

Project/Area Number 20590684
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionSaitama Medical University

Principal Investigator

HARA Masaaki  Saitama Medical University, 医学部, 講師 (50129160)

Co-Investigator(Kenkyū-buntansha) 高田 綾  埼玉医科大学, 医学部, 講師 (30245196)
斎藤 一之 (齋藤 一之)  埼玉医科大学, 医学部, 教授 (10215535)
Co-Investigator(Renkei-kenkyūsha) SAITO Kazuyuki  埼玉医科大学, 医学部, 教授 (10215535)
TAKADA Aya  埼玉医科大学, 医学部, 講師 (30245196)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2008: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsレーザーマイクロダイゼクション / 指紋 / STR / リアルタイムPCR / 指紋隆線 / DNA抽出 / レーザーマイクロダイゼクッション / 個人識別 / 物体検査
Research Abstract

The short tandem repeat (STR) genotyping of the extracted DNA from the single print of tape adhere the fingerprint (200mm^2) were performed, resulted in STR genotyping of the single print could be identified easily. Next study, to reveal the DNA extracted from refined single line of fingerprint on the microslide glass, STR genotyping of DNA sample from the single line was performed. A STR genotyping was identifiable 12~6 luci in 10x6mm, 7~2 loci in 7x4mm and 4~1 loci in 7x2mm.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (16 results)

All 2011 2010 2009 2008

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (7 results)

  • [Journal Article] A Simple Identification Method of Saliva by Detecting Streptococcus salivarius Using Loop-mediated Isothermal Amplification.2011

    • Author(s)
      Hiroaki Nakanishi, Takeshi Ohmori, Masaaki Hara, Aya Takada, Hideki Shojo, Noboru Adachi, Kazuyuki Saito
    • Journal Title

      JForencic Science 56 S1

      Pages: 158-161

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] クマネズミ胃内容物からのヒトSTR 型解析の検討2010

    • Author(s)
      原正昭, 高田綾, 斎藤一之, 木戸啓, 亀山浩, 山本靖久
    • Journal Title

      DNA 多型 Vol.18

      Pages: 144-147

    • NAID

      10029668204

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] クマネズミ胃内容物からのヒトSTR型解析の検討2010

    • Author(s)
      原正昭, 他5名
    • Journal Title

      DNA多型

      Volume: 18 Pages: 144-147

    • NAID

      10029668204

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アジア人3集団におけるDXS6789座位の構造多型2010

    • Author(s)
      永井淳, 他3名
    • Journal Title

      DNA多型

      Volume: 18 Pages: 191-193

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel method for the identification of saliva by detecting oral streptococci using PCR.2009

    • Author(s)
      Hiroaki Nakanishi, Akira Kido, Takeshi Ohmori, Aya Takada, Masaaki Hara, Noboru Adachi, Kazuyuki Saito
    • Journal Title

      Forensic Science International 183

      Pages: 20-23

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] STR genotyping of extracted from used Triage kits.2009

    • Author(s)
      Masaaki Hara, Akira Kido, Aya Takada, Takasi Miyazaki, Kazuyuki Saito
    • Journal Title

      Forensic Science International : Genetics Supplement Series 2

      Pages: 157-158

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sequence polymorphisms at the DXS6789, DXS8377 and DXS101 loci in Asian populations.2009

    • Author(s)
      Atsushi Nagai, Masaaki Hara, Akira Kido, Aya Takada, Kazuyuki Saito, Yasuo Bunai
    • Journal Title

      Forensic Science International : Genetics Supplement Series 2

      Pages: 49-50

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] STR and Y-STR genotyping assays of 25-year-old semen stains.2008

    • Author(s)
      M Hara, A Kido, H Kameyama, A Kido, T Miyazaki, K Saito
    • Journal Title

      Forensic Science International, Genetics Supplement Series 1

      Pages: 428-429

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel method for efficient analysis of STR loci from a single sperm captured by laser microdissection.2008

    • Author(s)
      T. Miyazaki, M.Hara, A. Ichiki, Y. Yamamoto, A.Takada, A.Kido, M. Nodera, H. Yanagisawa, H. Suzuki, K Saito
    • Journal Title

      Forensic Science International, Genetics Supplement Series 1

      Pages: 437-438

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] DXS10135 およびDXS10146 の構造多型2010

    • Author(s)
      永井淳
    • Organizer
      日本DNA 多型学会第19回学術集会
    • Place of Presentation
      静岡
    • Year and Date
      2010-11-19
    • Related Report
      2010 Final Research Report
  • [Presentation] クマネズミの食壊により顔面部のみが白骨化した一剖検例2010

    • Author(s)
      原正昭
    • Organizer
      第94次日本法医学会学術全国集会
    • Place of Presentation
      東京
    • Year and Date
      2010-06-25
    • Related Report
      2010 Final Research Report
  • [Presentation] クマネズミの食壊により顔面部のみが白骨化した一剖検例2010

    • Author(s)
      原正昭, 他6名
    • Organizer
      日本法医学会学術全国集会
    • Place of Presentation
      タワーホール船堀(東京)
    • Year and Date
      2010-06-25
    • Related Report
      2010 Annual Research Report
  • [Presentation] STR genotyping of extracted from used Triage kits.2009

    • Author(s)
      原正昭
    • Organizer
      23^<rd> World Congress. International Society for Forensic Genetics
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      2009-09-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Sequence polymorphisms at the DXS6789, DXS8377 and DXS101 loci in Asian populations.2009

    • Author(s)
      永井淳
    • Organizer
      23^<rd> World Congress. International Society for Forensic Genetics
    • Place of Presentation
      Buenos Aires, Argentina
    • Year and Date
      2009-09-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Genetic studies of eight X-STRs in the Japanese population.2008

    • Author(s)
      Tamura A
    • Organizer
      7^<th> International Symposium Advances in Legal Medicine.
    • Place of Presentation
      Osaka Japan
    • Year and Date
      2008-09-05
    • Related Report
      2010 Final Research Report
  • [Presentation] Genetic data on Y-chromosome STR loci in Japanese, Bangladeshis, and Indonesians using an AmpFlSTR Yfiler kit.2008

    • Author(s)
      原正昭
    • Organizer
      DNA in Forensics 2008
    • Place of Presentation
      Ancona Italy
    • Year and Date
      2008-05-30
    • Related Report
      2010 Final Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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