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Repair of damaged DNA and detection of its DNA polymorphisms

Research Project

Project/Area Number 20590687
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Legal medicine
Research InstitutionNihon University

Principal Investigator

TIE Jian  Nihon University, 医学部, 講師 (40277439)

Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
KeywordsDNA / 損傷 / 修復 / 多型 / 個人識別 / UV照射 / 多型検出 / 構造修復 / 修復酵素 / 法科学試料 / ダメージ / ARP / STR / ヒトDNA / 陳旧血痕 / STR多型
Research Abstract

The polymorphisms of short tandem(STR) repeat were successfully amplified from human bloodstain collected over the past 40 years and UV irradiated DNA by DNA repair. The damage of DNA was referenced by Aldehyde reactive probe, AP site of human cell DNA was increase after treated by UVC and DNA repair was performed for two days UV damage DNA, the AP site was decrease after DNA repair and STR was detected for all damaged samples.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (17 results)

All 2010 2009 2008

All Journal Article (10 results) (of which Peer Reviewed: 7 results) Presentation (5 results) Book (2 results)

  • [Journal Article] Direct PCR Amplification from Extracted Tissues for Individual Identification.2010

    • Author(s)
      Tie J, Uchigasaki S
    • Journal Title

      International Medical Journal. 17(4)

      Pages: 285-288

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct and rapid PCR amplification using digested tissues for the diagnosis of drowning.2010

    • Author(s)
      Tie J, Uchigasaki S
    • Journal Title

      Electrophoresis. 31

      Pages: 2411-2415

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genetic polymorphisms of Eight X-chromosomal STR Loci in the population of Japanese.2010

    • Author(s)
      Tie J, Uchigasaki S
    • Journal Title

      Forensic Sci Int : Genetics 4

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Direct and rapid PCR amplification using digested tissues for the diagnosis of drowning.2010

    • Author(s)
      Tie J, Uchigasaki S, et al.
    • Journal Title

      Electrophoresis

      Volume: 31 Pages: 2411-2415

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Direct PCR Amplification from Extracted Tissues for Individual Identification.2010

    • Author(s)
      Tie J, Uchigasaki S
    • Journal Title

      International Medical Journal.

      Volume: 17(4) Pages: 285-288

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic polymorphisms of Eight X-chromosomal STR Loci in the population of Japanese.2010

    • Author(s)
      Tie J, Uchigasaki S, et al.
    • Journal Title

      Forensic Sci Int : Genetics

      Volume: 4

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 小室歳信血痕からのAP siteの検出と修復について2009

    • Author(s)
      鉄堅、内ヶ崎西作、押田茂實、堤博文
    • Journal Title

      DNA多型 Vol.17

      Pages: 203-205

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 血痕から AP site の検出と修復について2009

    • Author(s)
      鉄堅
    • Journal Title

      DNA多型 Vol.17

      Pages: 203-205

    • Related Report
      2009 Annual Research Report
  • [Journal Article] A case of personal identification due to detection of rare DNA types from seminal stain2009

    • Author(s)
      堤博文
    • Journal Title

      J.Oral Scie. Vol.51(4)

      Pages: 645-650

    • Related Report
      2009 Annual Research Report
  • [Journal Article] SMAP法による歯髄からの性別判定2009

    • Author(s)
      堤博文
    • Journal Title

      DNA多型 Vol.17

      Pages: 22-25

    • Related Report
      2009 Annual Research Report
  • [Presentation] Quenching Probe法によるABO式遺伝子型検査及びRHD遺伝子検査2010

    • Author(s)
      堤博文・鉄堅
    • Organizer
      日本DNA多型学会
    • Place of Presentation
      三島
    • Year and Date
      2010-11-19
    • Related Report
      2010 Annual Research Report
  • [Presentation] Direct PCR Amplification from Extracted Tissues for Individual Identification2010

    • Author(s)
      Tie J
    • Organizer
      62^<th> Annual scientific meeting of American academy of forensic sciences
    • Place of Presentation
      Seattle.-USA
    • Year and Date
      2010-02-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] Direct PCR Amplification from Extracted Tissues for Individual Identification.2010

    • Author(s)
      Tie J, Uchigasaki S, Oshida S.
    • Organizer
      62th Annual scientific meeting of American academy of forensic sciences.
    • Place of Presentation
      Seattle.-USA
    • Year and Date
      2010-02-22
    • Related Report
      2010 Final Research Report
  • [Presentation] 血痕からのAP siteの検出と修復について2008

    • Author(s)
      鉄堅, 内ヶ崎西作, 押田茂實
    • Organizer
      日本DNA多型学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-21
    • Related Report
      2008 Annual Research Report
  • [Presentation] 押田茂實:血痕からのAP siteの検出と修復について2008

    • Author(s)
      鉄堅,内ヶ崎西作
    • Organizer
      第17回DNA多型学術集会
    • Place of Presentation
      東京
    • Year and Date
      2008-11-06
    • Related Report
      2010 Final Research Report
  • [Book] Q&A見てわかるDNA鑑定(DNA型鑑定の実際と問題点)2010

    • Author(s)
      押田茂實・岡部保男、鉄堅
    • Publisher
      現代人文社
    • Related Report
      2010 Final Research Report
  • [Book] Q&A見てわかるDNA鑑定(DNA型鑑定の実際と問題点)2010

    • Author(s)
      押田茂實・岡部保男
    • Total Pages
      177
    • Publisher
      現代人文社
    • Related Report
      2010 Annual Research Report

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Published: 2008-04-01   Modified: 2016-04-21  

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