Repair of damaged DNA and detection of its DNA polymorphisms
| Project/Area Number |
20590687
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Nihon University |
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Principal Investigator |
TIE Jian Nihon University, 医学部, 講師 (40277439)
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| Project Period (FY) |
2008 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | DNA / 損傷 / 修復 / 多型 / 個人識別 / UV照射 / 多型検出 / 構造修復 / 修復酵素 / 法科学試料 / ダメージ / ARP / STR / ヒトDNA / 陳旧血痕 / STR多型 |
|---|
| Research Abstract |
The polymorphisms of short tandem(STR) repeat were successfully amplified from human bloodstain collected over the past 40 years and UV irradiated DNA by DNA repair. The damage of DNA was referenced by Aldehyde reactive probe, AP site of human cell DNA was increase after treated by UVC and DNA repair was performed for two days UV damage DNA, the AP site was decrease after DNA repair and STR was detected for all damaged samples.
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Report
(4 results)
Research Products
(17 results)
