Study of molecular basis of motilin and growth hormone secretagogue receptors

• Project/Area Number: 20590727
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Ehime University
• Principal Investigator: MATSUURA Bunzo Ehime University, 大学院・医学系研究科, 寄附講座教授 (80284420)
• Research Collaborator: UEDA Teruhisa 愛媛大学, 大学院 ; NUNOI Hiroaki 愛媛大学, 大学院 ; MILLER LJ Mayo Clinic Arizona
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: モチリン / グレリン / モチリン受容体 / エリスロマイシン / トランスジェニックマウス / グレリン受容体 / キメラ受容体

Research Abstract

Motilin, erythromycin and ghrelin are important endogenous regulators of gastrointestinal motor function, mediated by the class A G protein-coupled motilin receptor and GHS receptor. We have identified new critical residues in the transmembrane domains of the motilin receptor for erythromycin binding and activity using receptor mutagenesis. We have also generated human motilin receptor transgenic mice, and clarified relationship between motilin receptor and GHS receptor.

Research Products

• [Journal Article] Mutational analysis of predicted extracellular domains of human growth hormone secretagogue receptor 1a. (2011)
  • Author(s): Ueda T, Matsuura B, Miyake T, Furukawa S, Abe M, Hiasa Y, Onji M
  • Journal Title: Regul Pept 166 Pages: 28-35
  • Peer Reviewed
• [Journal Article] Mutational analysis of predicted extracellular domains of human growth hormone secretagogue receptor la (2011)
  • Author(s): Ueda T, Matsuura B, Miyake T, Furukawa S, Abe M, Hiasa Y, Onji M
  • Journal Title: Regul Pept Volume: 166 Pages: 28-35
  • Peer Reviewed
• [Journal Article] 消化管ホルモン研究の再来 (2010)
  • Author(s): 松浦文三, 上田晃久, 宇都宮幸子, 布井弘明, 恩地森一
  • Journal Title: 愛媛医学 29 Pages: 7-13
• [Journal Article] Amino acid imbalance in patients with chronic liver diseases. (2010)
  • Author(s): Michitaka K, Hiraoka A, Kume M, Uehara T, Hidaka S, Ninomiya T, Haseba A, Miyamoto Y, Ichiryu M, Tanihira T, Nakahara H, Ochi H, Tanabe A, Uesugi K, Tokumoto Y, Mashiba T, Abe M, Hiasa Y, Matsuura B, Onji M
  • Journal Title: Hepatol Res 40 Pages: 393-398
  • NAID: 10027291605
  • Peer Reviewed
• [Journal Article] Impaired dendritic cell functions disrupt antigen-specific adaptive immune responses in mice with nonalcoholic fatty liver disease. (2010)
  • Author(s): Miyake T, Akbar SM, Yoshida O, Chen S, Hiasa Y, Matsuura B, Abe M, Onji M
  • Journal Title: J Gastroenterol 45 Pages: 859-867
  • NAID: 10026610917
  • Peer Reviewed
• [Journal Article] Impaired dendritic cell functions disrupt antigen-specific adaptive immune responses in mice with nonalcoholic fatty liver disease (2010)
  • Author(s): Miyake T, Akbar SM, Yoshida O, Chen S, Hiasa Y, Matsuura B, Abe M, Onji M
  • Journal Title: J Gastroenterol Volume: 45 Pages: 859-867
  • NAID: 10026610917
  • Peer Reviewed
• [Journal Article] Amino acid imbalance in patients with chronic liver diseases (2010)
  • Author(s): Michitaka K, Hiraoka A, Kume M, Uehara T, Hidaka S, Ninomiya T, Haseba A, Miyamoto Y, Ichiryu M, Tanihira T, Nakahara H, Ochi H, Tanabe A, Uesugi K, Tokumoto Y, Mashiba T, Abe M, Hiasa Y, Matsuura B, Onji M
  • Journal Title: Hepatol Res Volume: 40 Pages: 393-398
  • NAID: 10027291605
  • Peer Reviewed
• [Journal Article] 消化管ホルモン研究の再来 (2010)
  • Author(s): 松浦文三, 上田晃久, 宇都宮幸子, 布井弘明, 恩地森一
  • Journal Title: 愛媛医学 Volume: 29 Pages: 7-13
• [Journal Article] High orotate phosphoribosyltransferase gene expression predicts complete response to chemoradiotherapy in patients with squamous cell carcinoma of the esophagus. (2009)
  • Author(s): Kajiwara T, Nishina T, Hyodo I, Moriwaki T, Endo S, Nasu J, Hori S, Matsuura B, Hiasa Y, Onji M
  • Journal Title: Oncology 76 Pages: 342-349
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Konishi I, Hiasa Y, Shigematsu S, Hirooka M, Furukawa S, Abe M, Matsuura B, Michitaka K, Horiike N, Onji M
  • Journal Title: Liver Int 29 Pages: 1194-1201
  • Peer Reviewed
• [Journal Article] Changes of anthropometric and biological parameters in patients with non-alcoholic steatohepatitis for dietary modification. (2009)
  • Author(s): Toshimitsu K, Matsuura B, Nagai Y, Miyake T, Ueda T, Furukawa S, Abe M, Hiasa Y, Ebihara K, Onji M
  • Journal Title: J Metab Clin Nutr 12 Pages: 337-346
  • NAID: 10029098055
  • Peer Reviewed
• [Journal Article] Changes of anthropometric and biological parameters in patients with non-alcoholic steatohepatitis for dietary modification (2009)
  • Author(s): Toshimitsu K, Matsuura B, Nagai Y, Miyake T, Ueda T, Furukawa S, Abe M, Hiasa Y, Ebihara K, Onji M
  • Journal Title: J Metab Clin Nutr 12 Pages: 337-346
  • NAID: 10029098055
  • Peer Reviewed
• [Journal Article] Diabetes pattern on 75g oral glucose tolerance test is a risk factor for hepatocellular carcinoma in patients with hepatitis C virus (2009)
  • Author(s): Konishi I, Hiasa Y, Shigematsu S, Hirooka M, Furukawa S, Abe M, Matsuura B, Michitaka K, Horiike N, Onji M
  • Journal Title: Liver Int 29 Pages: 1194-1201
  • Peer Reviewed
• [Journal Article] High orotate phosphoribosyltransferase gene expression predicts complete response to chemoradiotherapy in patients with squamous cell carcinoma of the esophagus (2009)
  • Author(s): Kajiwara T, Nishina T, Hyodo I, Moriwaki T, Endo S, Nasu J, Hori S, Matsuura B, Hiasa Y, Onji M
  • Journal Title: Oncology 76 Pages: 342-349
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 松浦文三, 恩地森一
  • Journal Title: 清野裕, 門脇孝, 中村丁次, 本田佳子編:NST臨床栄養スタッフマニュアル(医学書院, 東京) Pages: 99-115
• [Journal Article] Wilms' tumor 1 gene expression is increased in hepatocellular carcinoma and associated with poor prognosis. (2008)
  • Author(s): Sera T, Hiasa Y, Mashiba T, Tokumoto Y, Hirooka M, Konishi I, Matsuura B, Michitaka K, Udaka K, Onji M
  • Journal Title: Eur J Cancer 44 Pages: 600-608
  • Peer Reviewed
• [Journal Article] NIS mRNA expression level in resected thyroid tissue as a marker of postoperative hypothyroidism after subtotal thyroidectomy in patients with Graves' disease. (2008)
  • Author(s): Goto A, Uchino S, Noguchi S, Wakiya S, Watanabe Y, Murakami T, Matsuura B, Onji M
  • Journal Title: Endocr J 55 Pages: 73-81
  • NAID: 10021264570
  • Peer Reviewed
• [Journal Article] Hepatitis C virus replication is inhibited by 22beta-methoxyolean 12-ene-3beta, 24 (4beta)-diol (ME3738) through enhancing interferon-beta. (2008)
  • Author(s): Hiasa Y, Kuzuhara H, Tokumoto Y, Konishi I, Yamashita N, Matsuura B, Michitaka K, Chung RT, Onji M
  • Journal Title: Hepatology 48 Pages: 59-69
  • Peer Reviewed
• [Journal Article] Mutational analysis of the predicted intracellular loop domains of the human motilin receptor. (2008)
  • Author(s): Tokunaga H, Matsuura B, Dong, M, Miller LJ, Ueda T, Furukawa S, Hiasa Y, Onji M
  • Journal Title: Am J Physiol-Gastrointest Liver Physiol 294 Pages: 460-466
  • Peer Reviewed
• [Journal Article] Mutational analysis of the predicted intracellular loop domains of the humanmotilin receptor. (2008)
  • Author(s): Tokunaga H, Matsuura B, Dong, M, Miller LJ, Ueda T, Furukawa S, Hiasa Y, Onji M
  • Journal Title: Am J Physiol-Gastrointest Liver Physiol 294
  • Peer Reviewed
• [Journal Article] Hepatitis C virus replication is inhibited by 22beta-methoxyolean-12-ene-3beta, 24(4beta)-diol(ME3738)through enhancing interferon-beta. (2008)
  • Author(s): Hiasa Y, Kuzuhara H, Tokumoto Y, Konishi I, Yamashita N, Matsuura B, Michitaka K, Chung RT, Onji M
  • Journal Title: Hepatology 48 Pages: 59-69
  • Peer Reviewed
• [Presentation] A cross-talk between motilin receptor and growth hormone secretagogue receptor using chimeric construct's expression and co-expression. (2010)
  • Author(s): Matsuura B, Utsunomiya S, Ueda T, Miyake T, Furukawa S, Murakami H, Abe M, Hiasa Y, Onji M
  • Organizer: AGA meeting 2010
  • Place of Presentation: New Orleans, USA.
• [Presentation] A cross-talk between motilin receptor and growth hormone secretagogue receptor using chimeric construct's expression and co-expression (2010)
  • Author(s): Matsuura B, Utsunomiya S, Ueda T, Miyake T, Furukawa S, Murakami H, Abe M, Hiasa Y, Onji M
  • Organizer: AGA meeting 2010
  • Place of Presentation: New Orleans, USA
• [Presentation] Critical residues in the transmembrane helical bundle domains of the human motilin receptor for erythromycin binding and activity. (2009)
  • Author(s): Matsuura B, Utsunomiya S, Ueda T, Dong M, Miller LJ, Miyake T, Furukawa S, Abe M, Murakami H, Onji M
  • Organizer: AGA meeting 2009
  • Place of Presentation: Chicago, USA.
• [Presentation] Critical residues in the transmembrane helical bundle domains of the human motilin receptor for erythromycin binding and activity (2009)
  • Author(s): Matsuura B, Utsunomiya S, Ueda T, Dong M, Miller LJ, Miyake T, Furukawa S, Abe M, Murakami H, Onji M
  • Organizer: AGA meeting 2009
  • Place of Presentation: Chicago America
• [Presentation] 消化管運動促進物質モチリン・エリスロマイシンのモチリン受容体におけるシグナル伝達系の解析 (2008)
  • Author(s): 松浦文三, 上田晃久, 恩地森一
  • Organizer: JDDW 2008
  • Place of Presentation: 東京
• [Presentation] Identification of peptide-ligand binding domains of the growth hormone secretagogue receptor. (2008)
  • Author(s): Ueda T, Matsuura B, Miyake T, Furukawa S, Hiasa Y, Murakami H, Onji M
  • Organizer: AGA meeting 2008
  • Place of Presentation: San Diego, USA.
• [Presentation] New critical residues in the predicted intracellular loop domains of human motilin receptor. (2008)
  • Author(s): Matsuura B, Ueda T, Dong M, Miller LJ, Miyake T, Furukawa S, Onji M
  • Organizer: AGA meeting 2008
  • Place of Presentation: San Diego, USA.
• [Presentation] New critical residues in the predicted intracellular loop domains of humanmotilin receptor (2008)
  • Author(s): Matsuura B, Ueda T, Dong M, Miller LJ, Miyake T, Furukawa S, Onji M
  • Organizer: AGA meeting 2008
  • Place of Presentation: San Diego, USA
• [Presentation] Identification of peptide-ligand binding domains of the growth hormonesecretagogue receptor (2008)
  • Author(s): Ueda T, Matsuura B, Miyake T, Furukawa S, Hiasa Y, Murakami H, Onji M
  • Organizer: AGA meeting 2008
  • Place of Presentation: San Diego, USA
• [Presentation] 消化管運動促進物質モチリン・エリスロマイシンのモチリン受容体におけるシグナル伝達系の解析 (2008)
  • Author(s): 松浦文三, 上田晃久, 恩地森一
  • Organizer: JDDW2008ワークショップ20
  • Place of Presentation: 東京
• [Book] 肝硬変(NASHを含む).(雨海照祥編:ワンステップ栄養アセスメント応用編) (2010)
  • Author(s): 松浦文三, 恩地森一
  • Publisher: 医歯薬出版,東京
• [Book] ワンステップアップ栄養アセスメント応用編(雨海照祥編) (2010)
  • Author(s): 松浦文三, 恩地森一
  • Publisher: 医歯薬出版,東京
• [Book] NST臨床栄養療法スタッフマニュアル(ダンピング症候群,便秘,黄疸)(清野裕,門脇孝,中村丁次,本田佳子編) (2009)
  • Author(s): 松浦文三, 恩地森一
  • Publisher: 医学書院,東京
• [Book] NASH診療best approach(食事療法)(西原利治編) (2008)
  • Author(s): 松浦文三, 恩地森一
  • Publisher: 中外医学社,東京
• [Book] 西原利治編 : NASH診療best approach. (2008)
  • Author(s): 松浦文三, 恩地森一
  • Publisher: 中外医学社, 東京
• [Remarks] ホームページ等
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• [Remarks]
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• [Remarks]
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