Development of diagnostic and therapeutic strategies for gastrointestinalcancer : focus on tumor-associated ribosomal proteins
| Project/Area Number |
20590746
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Sapporo Medical University |
|---|
Principal Investigator |
SASAKI Yasushi Sapporo Medical University, 医学部, 准教授 (70322328)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | リボゾーム蛋白質 / 消化器癌 / p53 / 抗癌剤感受性 / アポトーシス / リボソーム蛋白質 |
|---|
| Research Abstract |
We report here that L13 is activated in human gastrointestinal cancers. By using qRT-PCR, we analyzed expression of L13 in freshly resected cancer tissue of the stomach, colorectum, and liver. Upregulation of L13 mRNA expression was observed in 10 (28%) of 36 stomach, 19 (41%) of 46 colorectal, and 5 (20%) of 25 liver cancer tissue samples compared to adjacent normal tissue samples. We also found that increased expression of the L13 gene was correlated with clinical staging in stomach cancers. Importantly, we discovered that L13 expression was inversely related to p53 mutational status in primary gastric cancer, and siRNA-mediated L13 suppression resulted in robust induction of p53 protein. These findings suggest that L13 plays an essential role in the progression of some gastrointestinal malignancies by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53.
|
Report
(4 results)
Research Products
(52 results)
-
[Journal Article] Myogenic lineage differentiated mesenchymal stem cells enhance recovery from dextran sulfate sodium-induced colitis in the rat.2011
Author(s)
Tanaka H, Arimura Y, Yabana T, Goto A, Hosokawa M, Nagaishi K, Yamashita K, Yamamoto H, Sasaki Y, Fujimiya M, Imai K, Shinomura Y.
-
Journal Title
J Gastroenterol. 46
Pages: 143-152
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] IGFBP7 is a p53 responsive gene specifically silenced in colorectal cancer with CpG island methylator phenotype.2010
Author(s)
Suzuki H, Igarashi S, Nojima M, Maruyama R, Yamamoto E, Kai M, Akashi H, Watanabe Y, Yamamoto H, Sasaki Y, Itoh F, Imai K, Sugai T, Shen L, Issa JP, Shinomura Y, Tokino T, Toyota M.
-
Journal Title
Carcinogenesis 31
Pages: 342-349
Related Report
Peer Reviewed
-
[Journal Article] DNA methylation of interferon regulatory factors in gastric cancer and noncancerous gastric mucosae.2010
Author(s)
Yamashita M, Toyota M, Suzuki H, Nojima M, Yamamoto E, Kamimae S, Watanabe Y, Kai M, Akashi H, Maruyama R, Sasaki Y, Yamano H, Sugai T, Shinomura Y, Imai K, Tokino T, Itoh F.
-
Journal Title
Cancer Sci. 101
Pages: 1708-1716
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
[Journal Article] p53 Family Members Regulate the Expression of the Apolipoprotein D Gene.2009
Author(s)
Sasaki Y, Negishi H, Koyama R, Anbo N, Ohori K, Idogawa M, Mita H, Toyota M, Imai K, Shinomura Y, Tokino T.
-
Journal Title
J Biol Chem. 284
Pages: 872-883
Related Report
Peer Reviewed
-
-
-
-
-
[Journal Article] A novel method, digital genome scanning detects KRAS gene amplification in gastric cancers : involvement of overexpressed wild-type KRAS in downstream signaling and cancer cell growth.2009
Author(s)
Mita H, Toyota M, Aoki F, Akashi H, Maruyama R, Sasaki Y, Suzuki H, Idogawa M, Kashima L, Yanagihara K, Fujita M, Hosokawa M, Kusano M, Tatsumi H, Imai K, Shinomura Y, Tokino T.
-
Journal Title
BMC Cancer. 9
Pages: 198-198
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
[Journal Article] Identification of flotillin-2, a major protein on lipid rafts, as a novel target of the p53 family members.2008
Author(s)
Sasaki Y, Oshima Y, Koyama R, Maruyama R, Akashi H, Mita H, Toyota M, Shinomura Y, Imai K, Tokino T.
-
Journal Title
Mol Cancer Res. 6
Pages: 395-406
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
[Presentation] hCLCA2, a p53 inducible transmembrane protein regulates cancer cell migration and adhesion.2010
Author(s)
佐々木泰史, 横田育子, 鹿島理沙, 井戸川雅史, 鈴木拓, 豊田実, 今井浩三, 篠村恭久, 時野隆至
Organizer
8th AACR-JCA Joint International Conference.
Place of Presentation
Waikoloa, Hawaii
Year and Date
2010-02-08
Related Report
-
-
-
-
-
-
[Presentation] p53 family members regulate the expression of the apolipoprotein D gene.2009
Author(s)
Sasaki Y, Negishi H, Koyama R, Anbo N, Yokota I, Ohori K, Idogawa M, Mita H, Toyota M, Imai K, Shinomura Y, Tokino T
Organizer
American Association of Cancer Research (AACR) Annual meeting.
Place of Presentation
Denver, USA
Year and Date
2009-04-20
Related Report
-
-
-
-
-
-
-
