Defective autophagy system against bacteria in Crohn's macrophages

• Project/Area Number: 20590750
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Keio University
• Principal Investigator: SUSUMU Okamoto Keio University, 医学部, 講師 (70255446)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: クローン病 / オートファジー / マクロファージ

Research Abstract

The hypothesis of this study was that intestinal macrophages from Crohn's disease may have defect in autophagy system against intestinal bacteria. I performed immunohistochemistry and western blotting for samples both from human and murine Crohn's inflamed intestine using anti-LC-3 antibody. Disappointingly, I could not show any significant difference between normal control and Crohn's specimens in any series of experiments performed in this term. Now I am considering whether other experimental systems such as retroviral vectors or genetically engineered mice may be worth testing.

Research Products

• [Journal Article] Competition between colitogenic Th1 and Th17 cells contributes to the amelioration of colitis. (2010)
  • Author(s): Mikami Y, Kanai T, Sujino T, Ono Y, Hayashi A, Okazawa A, Kamada N, Matsuoka K, Hisamatsu T, Okamoto S, Takaishi H, Inoue N, Ogata H, Hibi T
  • Journal Title: Eur J Immunol. 40(9) Pages: 2409-22
  • Peer Reviewed
• [Journal Article] Imbalance of NKp44(+)NKp46(-) and NKp44(-)NKp46(+) natural killer cells in the intestinal mucosa of patients with Crohn's disease. (2010)
  • Author(s): Takayama T, Kamada N, Chinen H, Okamoto S, Kitazume MT, Chang J, Matuzaki Y, Suzuki S, Sugita A, Koganei K, Hisamatsu T, Kanai T, Hibi T
  • Journal Title: Gastroenterology. 139(3)
  • Peer Reviewed
• [Journal Article] Rapid endoscopic improvement is important for 1-year avoidance of colectomy but not for the long-term prognosis in cyclosporine A treatment for ulcerative colitis. (2010)
  • Author(s): Kobayashi T, Naganuma M, Okamoto S, Hisamatsu T, Inoue N, Ichikawa H, Takayama T, Saito R, Sujino T, Ogata H, Iwao Y, Hibi T
  • Journal Title: J Gastroenterol. 45(11) Pages: 1129-37
  • NAID: 10027699761
  • Peer Reviewed
• [Journal Article] Novel endoscopic activity index is useful for choosing treatment in severe active ulcerative colitis patients. (2010)
  • Author(s): Naganuma M, Ichikawa H, Inoue N, Kobayashi T, Okamoto S, Hisamatsu T, Kanai T, Ogata H, Iwao Y, Hibi T
  • Journal Title: J Gastroenterol. 45(9) Pages: 936-43
  • NAID: 10027698991
  • Peer Reviewed
• [Remarks] ホームページ等