Molecular Screening by Microarray analysis for Hepatocarcinogenesis.

• Project/Area Number: 20590752
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hokkaido University
• Principal Investigator: CHUMA Makoto Hokkaido University, 病院, 助教 (30360910)
• Co-Investigator(Kenkyū-buntansha): KOBAYASHI Takahiko 北海道大学, 大学院・医学研究科, 客員研究員 (80333607)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2009: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2008: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 癌進展 / RNA結合タンパク / RBM5 / マイクロアレイ / 網羅的探索 / 肝癌発生

Research Abstract

The expression of the RBM5 mRNA is decreased in cancer tissues. Overexpression of RBM5 enhanced p53-mediated inhibition of cancer cell growth and colony formation. RBM5 affected p53 transcriptional activity and levels of mRNA and protein for endogenous p53 target genes. RBM5 influenced protein, but not mRNA, levels of endogenous p53 suggesting that RBM5 contributes to p53 activity through post-transcriptional mechanisms. Our results show that RBM5 contributes to p53 transcriptional activity, and that growth suppression and apoptosis mediated by RBM5 are linked to activity of the tumor suppressor protein p53.

Research Products

• [Journal Article] p53 transactivation is involved in the antiproliferative activity of the putative tumor suppressor RBM5. (2011)
  • Author(s): Kobayashi T, Ishida J, Musashi M, Ota S, Yoshida T, Shimizu Y, Chuma M, Kawakami H, Asaka M, Tanaka J, Imamura M, Kobayashi M, Itoh H, Edamatsu H, Sutherland LC, Brachmann RK.
  • Journal Title: Int J Cancer. 128(2) Pages: 304-318
  • Peer Reviewed
• [Journal Article] p53 transactivation is involved in the antiproliferative activity of the putative turnor suppressor RBM5. (2011)
  • Author(s): Kobayashi T
  • Journal Title: Int J Cancer Volume: 128 Pages: 304-318
  • Peer Reviewed
• [Journal Article] Sensitive assay for quantification of hepatitis B virus mutants by use of a minor groovebinder probe and peptide nucleic acids. (2010)
  • Author(s): Hige S
  • Journal Title: J Cli Microbiol Volume: 48 Pages: 4487-4494
  • Peer Reviewed
• [Journal Article] The influence of hepatitis B DNA level and antiviral therapy on recurrence after initial curative treatment in patients with hepatocellular carcinoma. (2009)
  • Author(s): Chuma M, Hige S, Kamiyama T, Meguro T, Nagasaka A, Nakanishi K, Yamamoto Y, Nakanishi M, Kohara T, Sho T, Yamamoto K, Horimoto H, Kobayashi T, Yokoo H, Matsushita M, Todo S, Asaka M.
  • Journal Title: J Gastroenterol. 44(9) Pages: 991-999
  • NAID: 10025581551
  • Peer Reviewed
• [Journal Article] The influence of hepatitis B DNA level and antiviral therapy on recurrence afterinitial curative treatment in patients with hepatocellular carcinoma. (2009)
  • Author(s): Chuma M
  • Journal Title: J Gastroenterol. 23 Pages: 991-999
  • NAID: 10025581551
  • Peer Reviewed
• [Journal Article] Immunological and mutagenic actions of ribavirin monotherapy preceding combination therapy with interferon for patients with chronic hepatitis C. (2009)
  • Author(s): Ogawa K
  • Journal Title: Antivir Ther. 14 Pages: 513-522
  • Peer Reviewed
• [Journal Article] 8-OHdG is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection. (2008)
  • Author(s): Chuma M, Hige S, Nakanishi M, Ogawa K, Natsuizaka M, Yamamoto Y, Asaka M.
  • Journal Title: J Gastroenterol Hepatol. 23(9) Pages: 1431-1436
  • Peer Reviewed
• [Journal Article] 8-OHdG is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection (2008)
  • Author(s): Chuma M
  • Journal Title: J Gastroenterol Hepatol. 23 Pages: 1431-1436
  • Peer Reviewed
• [Journal Article] Abscopal effect on hepatocellular carcinoma (2008)
  • Author(s): Nakanishi M
  • Journal Title: Am J Gastroenterol 103 Pages: 1320-1321
  • Peer Reviewed
• [Presentation] 肝細胞癌根治後の再発に関するHBV-DNA量、抗ウイルス薬の影響 (2010)
  • Author(s): 中馬誠
  • Organizer: 第38回日本肝臓学会東部会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-02
• [Presentation] 肝細胞癌根治後の再発に関するHBV-DNA量、抗ウイルス薬の関与 (2009)
  • Author(s): 中馬誠
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸
  • Year and Date: 2009-06-05
• [Presentation] The Influence of Hepatitis B DNA Level and Antiviral Therapy on Recurrence After Initial Curative Treatment in Patients With Hepatocellular Carcinoma. (2009)
  • Author(s): Chuma M.
  • Organizer: 第19回 アジア太平洋肝臓学会
  • Place of Presentation: 香港
  • Year and Date: 2009-02-15
• [Presentation] 8-OHdG is a risk factor for development of hepatocellular carcinoma in patients with chronic hepatitis C virus infection. (2008)
  • Author(s): Chuma M.
  • Organizer: 第43回欧州肝臓学会
  • Place of Presentation: ミラノ
  • Year and Date: 2008-04-25
• [Presentation] 8-OHdG is a risk factor for development of hepatocellular carcinoma in natients with chronic hepatitis C virus infection (2008)
  • Author(s): Chuma M
  • Organizer: 第43回欧州肝臓学会
  • Place of Presentation: イタリア・ミラノ