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The development of a quantitative assay of a single-nucleotide mutant of drug-resistant hepatitis virus.

Research Project

Project/Area Number 20590753
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionHokkaido University

Principal Investigator

HIGE Shuhei  Hokkaido University, 病院, 講師 (70261295)

Co-Investigator(Kenkyū-buntansha) CHUUMA Makoto  北海道大学, 病院, 助教 (30360910)
Project Period (FY) 2008 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords肝炎ウイルス / 薬剤耐性 / 変異株 / 変異株定量 / 核酸アナログ / MGBプローブ / ペプチド核酸 / real-time PCR / real-time PCR法
Research Abstract

The most serious problem for patients with chronic hepatitis B who are administered with nucleotide analogues is the emergence of a resistant mutant virus and the occurrence of breakthrough hepatitis. Since hepatitis virus exists in a mixed population with multiple mutant strains, it has been difficult to measure each mutant strain accurately so far. In this study, a new sensitive method for quantification of a single-base mutant within the mixed population has been developed. This assay raised the possibility to have versatility as a sensitive method for the quantification of a single nucleotide mutation in a heterogeneous population.

Report

(4 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • 2008 Annual Research Report
  • Research Products

    (5 results)

All 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) Remarks (1 results)

  • [Journal Article] Sensitive Assay for Quantification of Hepatitis B Virus Mutants by Use of a Minor Groove Binder Probe and Peptide Nucleic Acids.2010

    • Author(s)
      Shubei Hige, Yoichi Yamamoto, Shigeru Yoshida, Tomoe Kobayashi, Hiromasa Horimoto, Keiko Yamamoto, Takuya Sho, Mitsuteru Natsuizaka, Mitsuru Nakanishi, Makoto Chuma, Masahiro Asaka
    • Journal Title

      Journal of Clinical Microbiology. 48

      Pages: 4487-4494

    • NAID

      120003043348

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sensitive assay for the quantification of hepatitis B virus mutants by a minor goove binder probe and petide nucleic acids2010

    • Author(s)
      髭修平
    • Journal Title

      Journal of Clinical Microbiology

      Volume: 48 Pages: 4497-4494

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Quantitative measurements of HBV mutants against lamivudine.2009

    • Author(s)
      Shuhei Hige
    • Organizer
      USJMSCP- NIH-APASL Joint Symposium. 19th Annual meeting of the Asian Pacific Association for the study of the liver.
    • Place of Presentation
      Hong Kong, China.
    • Year and Date
      2009-02-16
    • Related Report
      2010 Final Research Report
  • [Presentation] Quantitative measurempnts of HBV mutants against lamivudine2009

    • Author(s)
      髭修平
    • Organizer
      第19回アジア太平洋肝臓学会(APASL)USJMSCP-NIH-APASL Joint Symposium on HBV drug Resistance
    • Place of Presentation
      香港(中国)
    • Year and Date
      2009-02-16
    • Related Report
      2008 Annual Research Report
  • [Remarks] 北海道大学学術成果コレクション(略称:HUSCAP)ホームページ

    • URL

      http://eprints.lib.hokudai.ac.jp

    • Related Report
      2010 Final Research Report

URL: 

Published: 2008-04-01   Modified: 2016-04-21  

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