| Project/Area Number |
20590753
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
|
|---|
| Research Institution | Hokkaido University |
|---|
Principal Investigator |
HIGE Shuhei Hokkaido University, 病院, 講師 (70261295)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
CHUUMA Makoto 北海道大学, 病院, 助教 (30360910)
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 肝炎ウイルス / 薬剤耐性 / 変異株 / 変異株定量 / 核酸アナログ / MGBプローブ / ペプチド核酸 / real-time PCR / real-time PCR法 |
|---|
| Research Abstract |
The most serious problem for patients with chronic hepatitis B who are administered with nucleotide analogues is the emergence of a resistant mutant virus and the occurrence of breakthrough hepatitis. Since hepatitis virus exists in a mixed population with multiple mutant strains, it has been difficult to measure each mutant strain accurately so far. In this study, a new sensitive method for quantification of a single-base mutant within the mixed population has been developed. This assay raised the possibility to have versatility as a sensitive method for the quantification of a single nucleotide mutation in a heterogeneous population.
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