The development of a quantitative assay of a single-nucleotide mutant of drug-resistant hepatitis virus.

• Project/Area Number: 20590753
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Hokkaido University
• Principal Investigator: HIGE Shuhei Hokkaido University, 病院, 講師 (70261295)
• Co-Investigator(Kenkyū-buntansha): CHUUMA Makoto 北海道大学, 病院, 助教 (30360910)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000); Fiscal Year 2009: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2008: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 肝炎ウイルス / 薬剤耐性 / 変異株 / 変異株定量 / 核酸アナログ / MGBプローブ / ペプチド核酸 / real-time PCR / real-time PCR法

Research Abstract

The most serious problem for patients with chronic hepatitis B who are administered with nucleotide analogues is the emergence of a resistant mutant virus and the occurrence of breakthrough hepatitis. Since hepatitis virus exists in a mixed population with multiple mutant strains, it has been difficult to measure each mutant strain accurately so far. In this study, a new sensitive method for quantification of a single-base mutant within the mixed population has been developed. This assay raised the possibility to have versatility as a sensitive method for the quantification of a single nucleotide mutation in a heterogeneous population.

Research Products

• [Journal Article] Sensitive Assay for Quantification of Hepatitis B Virus Mutants by Use of a Minor Groove Binder Probe and Peptide Nucleic Acids. (2010)
  • Author(s): Shubei Hige, Yoichi Yamamoto, Shigeru Yoshida, Tomoe Kobayashi, Hiromasa Horimoto, Keiko Yamamoto, Takuya Sho, Mitsuteru Natsuizaka, Mitsuru Nakanishi, Makoto Chuma, Masahiro Asaka
  • Journal Title: Journal of Clinical Microbiology. 48 Pages: 4487-4494
  • NAID: 120003043348
  • Peer Reviewed
• [Journal Article] Sensitive assay for the quantification of hepatitis B virus mutants by a minor goove binder probe and petide nucleic acids (2010)
  • Author(s): 髭修平
  • Journal Title: Journal of Clinical Microbiology Volume: 48 Pages: 4497-4494
  • Peer Reviewed
• [Presentation] Quantitative measurements of HBV mutants against lamivudine. (2009)
  • Author(s): Shuhei Hige
  • Organizer: USJMSCP- NIH-APASL Joint Symposium. 19th Annual meeting of the Asian Pacific Association for the study of the liver.
  • Place of Presentation: Hong Kong, China.
  • Year and Date: 2009-02-16
• [Presentation] Quantitative measurempnts of HBV mutants against lamivudine (2009)
  • Author(s): 髭修平
  • Organizer: 第19回アジア太平洋肝臓学会(APASL)USJMSCP-NIH-APASL Joint Symposium on HBV drug Resistance
  • Place of Presentation: 香港(中国)
  • Year and Date: 2009-02-16
• [Remarks] 北海道大学学術成果コレクション(略称:HUSCAP)ホームページ
  • URL: http://eprints.lib.hokudai.ac.jp