Control of chronic hepatitis by splicing variant of hepatitis B virus

• Project/Area Number: 20590763
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: The University of Tokyo
• Principal Investigator: YOTSUYANAGI Hiroshi The University of Tokyo, 医学部附属病院, 准教授 (30251234)
• Project Period (FY): 2008 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2008: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: HBVコア遺伝子 / Splicing Variant / アミノ酸置換

Research Abstract

1. Nucleic or amino acid substitutions in the core gene of hepatitis B virus (HBV) core region were studied. (1) Smaller number of nucleic acid substitutions in the basic core promoter region was associated with inactivation of hepatitis. (2) Larger number of substituted amino acid in the core region was associated with inactivation of hepatitis.
2. The amino acid sequences of HBV splicing variant clones were analyzed. The majority of HBV clones had deletions spanning from end of core to preS-s regions (nt.2448～2934、3019～488). Six splicing patterns were found in the clones, three of which had large deletion (67AA, 87AA) in the core region.

Research Products

• [Journal Article] Effect of nucleoside analog-interferon sequential therapy on patients with acute exacerbation of chronic hepatitis B (2010)
  • Author(s): Okuse C, Yotsuyanagi H, et al.
  • Journal Title: Hepatol Res. Volume: 40 Pages: 461-469
  • NAID: 10026391897
  • Peer Reviewed
• [Journal Article] B型急性肝炎におけるHBs抗原陽性持続期間の検討 (2010)
  • Author(s): 山田典栄、四柳宏, 他
  • Journal Title: 肝臓 Volume: 51 Pages: 534-535
  • NAID: 10027469792
  • Peer Reviewed
• [Journal Article] Nucleotide and amino acid sequences of the basic core promoter, precore and core regions and the outcome of persistent genotype C hepatitis B viral infection with antibody to e antigen
  • Author(s): Yamada N, Yotsuyanagi H, Shintani Y, Koike K, et al.
  • Journal Title: J.Hepatol
• [Journal Article] Nucleotide and amino acid sequences of the basic core promoter, precore and core regions and the outcome of persistent genotype C hepatitis B viral infection with antibody to e antigen
  • Author(s): Yamada N, Yotsuyanagi H, Shintani Y, Koike K, et al.
  • Journal Title: J. Hepatol (accepted)
  • Peer Reviewed
• [Presentation] B型肝炎ウイルス遺伝子変異の病態・治療に及ぼす影響ウイルスゲノム中の欠失はB型慢性肝疾患の経過に大きな影響を及ぼす (2010)
  • Author(s): 山田典栄、四柳宏, 他
  • Organizer: 第46回日本肝臓学会総会
  • Place of Presentation: 山形市
  • Year and Date: 2010-05-27
• [Presentation] B型慢性肝疾患におけるB型肝炎ウイルス欠矢変異 (2009)
  • Author(s): 山田典栄, 四柳宏, ら
  • Organizer: 第45回日本肝臓学会総会
  • Place of Presentation: 神戸市
  • Year and Date: 2009-06-03
• [Presentation] Large deletion mutant of hepatitis B virus with amino acid substitutions in the core region emerges and dominates around the onset of active hepatitis in persistent HBV carriers (2008)
  • Author(s): Yamada N, Yotsuyanagi H, Shintani Y, Koike K, et al.
  • Organizer: アメリカ肝臓学会Annual Meeting.
  • Place of Presentation: サンフランシスコ.米国
  • Year and Date: 2008-11-02
• [Presentation] HBe抗原陽性B型慢性肝疾患におけるB型肝炎ウイルス欠失変異 (2008)
  • Author(s): 山田典栄, 四柳宏, 新谷良澄, 小池和彦, 他
  • Organizer: 日本肝臓学会総会
  • Place of Presentation: 松山市
  • Year and Date: 2008-06-05
• [Presentation] Large deletion mutant of hepatitis B virus with amino acid substitutions in the core region emerges and dominates around the onset of active hepatitis in persistent HBV carriers (2008)
  • Author(s): Yamada N, Yotsuyanagi H, Shintani Y, Koike K et al.
  • Organizer: アメリカ肝臓学会Annual Meeting
  • Place of Presentation: サンフランシスコ
• [Remarks] ホームページ等