Identification of the host lipid raft protein which interacts with hepatitis C virus protein
Project/Area Number |
20590767
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Gastroenterology
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Research Institution | University of Yamanashi |
Principal Investigator |
INOUE Taisuke University of Yamanashi, 大学院・医学工学総合研究部, 講師 (90422691)
|
Co-Investigator(Kenkyū-buntansha) |
ENOMOTO Nobuyuki 山梨大学, 大学院・医学工学総合研究部, 教授 (20251530)
|
Project Period (FY) |
2008 – 2010
|
Project Status |
Completed (Fiscal Year 2010)
|
Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | C型肝炎ウイルス / HCV NS5B / α-tubulin / 複製複合体 / プロテオミクス解析 / Tandem affinity purification / Tandem mass spectrometry / siRNA |
Research Abstract |
Hepatitis C virus (HCV) non structural proteins and host proteins are believed to be involved in HCV replication complex on lipid raft. To identify these host proteins that interact with HCV proteins, we employed the proteomics approach, combination of tandem affinity purification (TAP) method and mass spectrometry analysis. α-tubulin was identified as a novel HCV NS5B binding protein. Interaction between these two proteins was confirmed by the immunohistchemistry study and the co-immunoprecipitation study. To examine the effect of the interaction between these two proteins, α-tubulin expression was suppressed by siRNA on the HCV replicon stably expressing cells. By real-time PCR method, the HCV replicon RNA level was suppressed to 60% on the α-tubulin knock down cells. These results suggest that α-tubulin interacts with HCV NS5B protein and this interaction may be important for the HCV RNA replication. Further studies to identify direct interacting domain are required.
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Report
(4 results)
Research Products
(6 results)
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[Journal Article] Analysis of the complete open reading frame of hepatitis C virus in genotype 2a infection reveals critical sites influencing the response to peginterferon and ribavirin therapy.2011
Author(s)
Kadokura M, Maekawa S, Sueki R, Miura M, Komase K, Shindo H, Amemiya F, Uetake T, Inoue T, Sakamoto M, Nakagawa M, Sakamoto N, Watanabe M, Enomoto N.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] DICKKOPF-4 and -2 genes are upregulated in human colorectal cancer.2009
Author(s)
Matsui A, Yamaguchi T, Markawa S, Miyazaki C, Takano S, Uetake T, Inoue T, Otaka M, Otsuka H, Sato T, Yamashita A, Takahashi Y, Enomoto N.
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Journal Title
Cancer Sci. 100(10)
Pages: 1923-1930
NAID
Related Report
Peer Reviewed
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[Journal Article] Targeting lipid metabolism in the treatment of hepatitis C virus infection.2008
Author(s)
Amemiya F, Maekawa S, Itakura Y, Kanayama A, Matsui A, Takano S, Yamaguchi T, Itakura J, Kitamura T, Inoue T, Sakamoto M, Yamauchi K, Okada S, Yamashita A, Sakamoto N, Itoh M, Enomoto N.
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Journal Title
J Infect Dis. 197(3)
Pages: 631-370
Related Report
Peer Reviewed
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