| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2008: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Research Abstract |
Hepatitis C virus (HCV) non structural proteins and host proteins are believed to be involved in HCV replication complex on lipid raft. To identify these host proteins that interact with HCV proteins, we employed the proteomics approach, combination of tandem affinity purification (TAP) method and mass spectrometry analysis. α-tubulin was identified as a novel HCV NS5B binding protein. Interaction between these two proteins was confirmed by the immunohistchemistry study and the co-immunoprecipitation study. To examine the effect of the interaction between these two proteins, α-tubulin expression was suppressed by siRNA on the HCV replicon stably expressing cells. By real-time PCR method, the HCV replicon RNA level was suppressed to 60% on the α-tubulin knock down cells. These results suggest that α-tubulin interacts with HCV NS5B protein and this interaction may be important for the HCV RNA replication. Further studies to identify direct interacting domain are required.
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