| Project/Area Number |
20590786
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Kagoshima University |
|---|
Principal Investigator |
UTO Hirofumi Kagoshima University, 医歯学総合研究科, 講師 (20347058)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
MORIUCHI Akihiro 鹿児島大学, 医学部・歯学部附属病院, 助教 (40359823)
|
|---|
| Research Collaborator |
SATO Yuko 宮崎県産業支援財団, 研究員
TANOUE Shiro 鹿児島大学, 医歯学総合研究科, 大学院生
KUMAMTO Ryo 鹿児島大学, 医歯学総合研究科, 大学院生
ARIMA Shiho 鹿児島大学, 医歯学総合研究科, 大学院生
HAMABE Ayaka 鹿児島大学, 医歯学総合研究科, 大学院生
IBUSUKI Rie 鹿児島大学, 医歯学総合研究科, 大学院生
|
|---|
| Project Period (FY) |
2008 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2008: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Keywords | NASH / NAFLD / 酸化ストレス / MnSOD / フルクトース / 肝再生 / 肝発癌 / SNAFLD / 高血圧 / 脂質代謝異常 / 糖代謝異常 / kininogen / NBS標識法 |
|---|
| Research Abstract |
First of all, we explored the oxidative stress marker (s) using proteomics and identified that MnSOD may be a useful serum biomarker for distinguishing between simple steatotis (SS) and nonalcoholic steatohepatitis (NASH) in patients with nonalcoholic fatty liver disease (NAFLD). Second, in a rat experimental model, hepatic steatosis (HS) developed in the high fructose diet (H-fruc) group, but was less severe compared to the high fat diet (H-fat) group. In contrast, liver regeneration after 70% partial hepatectomy (PHx) was impaired in the H-fruc group compared to the H-fat and control groups. In addition, the H-fruc group developed a significantly higher incidence of GST-P-positive precancerous lesions compared to the H-fat group. These results indicate that impairment of liver regeneration after PHx and the incidence of precancerous lesions in HS are related to the cause, rather than the degree, of steatosis. Thus, our study demonstrates that serum MnSOD is potential novel marker for NAFLD, and that the pathophysiological condition and progression of NAFLD are potentially affected by different diets.
|
