| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2008: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
Cancer related materials, i.e., cell lines, endoscopic biopthy specimen, or, surgically resected specimen, were analyzed for ; i) expression of cancer stem cell markers, ii) tumor formation, iii) colony forming activity, iv) drug-resistance, and v) potentials for local invasion and metastasis. Our results suggest that side population (SP) cells isolated from pancreatic cancer have some cancer stem cell-like properties ; i) high tumorigenic/colony forming activity, ii) resistance to some chemotherapeutic drugs as well as apoptosis-inducing molecules such as TRAIL, iii) high metstatic potential, and iv) enhanced capability to undergo epithelial-to-mesenchymal transition (EMT) and invasion. In hepatocellular carcinoma, CD133-positive side population cells were highly tumorigenic and chemotherapy-resistant. These cancer stem cell-like features were enhanced by co-culturing with bone marrow-derived mesenchymal stem cells (MSCs) and/or MSC-derived myofibroblast-like cells, suggesting MSCs provide microenvironment so-called cancer stem cell niche to maintain stem cell phenotype in cancer derived SP cells. These results suggest that SP cells would be a therapeutic tagrget, especially to prevent cancer sten cell invasion and metastasis. Mesenchymal cell-derived microenvironment would be another terapeutic target to regulate cancer stem cell maintenance.
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